Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration
Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 10000...
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Format: | Article |
Language: | English |
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Dove Medical Press
2020-08-01
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Series: | Diabetes, Metabolic Syndrome and Obesity |
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Online Access: | https://www.dovepress.com/down-regulated-mir-21-in-gestational-diabetes-mellitus-placenta-induce-peer-reviewed-article-DMSO |
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author | Guan CY Tian S Cao JL Wang XQ Ma X Xia HF |
author_facet | Guan CY Tian S Cao JL Wang XQ Ma X Xia HF |
author_sort | Guan CY |
collection | DOAJ |
description | Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People’s Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xu Ma; Hong-Fei XiaReproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of ChinaTel +86-10-62178932Fax +86-10-62179059Email xuma_genetics@163.com; hongfeixia@126.comPurpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-α |
first_indexed | 2024-04-10T17:58:09Z |
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institution | Directory Open Access Journal |
issn | 1178-7007 |
language | English |
last_indexed | 2024-04-10T17:58:09Z |
publishDate | 2020-08-01 |
publisher | Dove Medical Press |
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series | Diabetes, Metabolic Syndrome and Obesity |
spelling | doaj.art-38f66cb3d5484835bfc3ead362c0c1d92023-02-02T16:52:24ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072020-08-01Volume 133009303456524Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and InfiltrationGuan CYTian SCao JLWang XQMa XXia HFChun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People’s Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xu Ma; Hong-Fei XiaReproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of ChinaTel +86-10-62178932Fax +86-10-62179059Email xuma_genetics@163.com; hongfeixia@126.comPurpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-αhttps://www.dovepress.com/down-regulated-mir-21-in-gestational-diabetes-mellitus-placenta-induce-peer-reviewed-article-DMSOgestational diabetes mellitusmirna-21insulin resistanceppar-α |
spellingShingle | Guan CY Tian S Cao JL Wang XQ Ma X Xia HF Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration Diabetes, Metabolic Syndrome and Obesity gestational diabetes mellitus mirna-21 insulin resistance ppar-α |
title | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
title_full | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
title_fullStr | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
title_full_unstemmed | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
title_short | Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
title_sort | down regulated mir 21 in gestational diabetes mellitus placenta induces ppar alpha to inhibit cell proliferation and infiltration |
topic | gestational diabetes mellitus mirna-21 insulin resistance ppar-α |
url | https://www.dovepress.com/down-regulated-mir-21-in-gestational-diabetes-mellitus-placenta-induce-peer-reviewed-article-DMSO |
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