Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an <named-content content-type="genus-species">Escherichia coli</named-content> NADPH-Auxotrophic Strain

Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an <named-content content-type="genus-species">Escherichia coli</named-content> NADPH-Auxotrophic Strain

ABSTRACT The nicotinamide cofactor specificity of enzymes plays a key role in regulating metabolic processes and attaining cellular homeostasis. Multiple studies have used enzyme engineering tools or a directed evolution approach to switch the cofactor preference of specific oxidoreductases. However...

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Bibliographic Details
Main Authors: Madeleine Bouzon, Volker Döring, Ivan Dubois, Anne Berger, Gabriele M. M. Stoffel, Liliana Calzadiaz Ramirez, Sophia N. Meyer, Marion Fouré, David Roche, Alain Perret, Tobias J. Erb, Arren Bar-Even, Steffen N. Lindner
Format: Article
Language:English
Published: American Society for Microbiology 2021-08-01
Series:mBio
Subjects:
evolution
NADPH
metabolism
NADPH-auxotrophic strain
ALE
oxidoreductases
Online Access:https://journals.asm.org/doi/10.1128/mBio.00329-21

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