Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer
<p>Abstract</p> <p>Background</p> <p>β-catenin is a multifunctional protein involved in two apparently independent processes: cell-cell adhesion and signal transduction. β-catenin is involved in Wnt signaling pathway that regulates cellular differentiation and prolifera...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-02-01
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| Series: | World Journal of Surgical Oncology |
| Online Access: | http://www.wjso.com/content/5/1/21 |
| _version_ | 1818021103549808640 |
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| author | Shinoda Noriyuki Sugiura Hironori Terashita Yukio Shibata Yasuyuki Ishiguro Hideyuki Haruki Nobuhiro Nishiwaki Tadashi Kudo Junzo Kimura Masahiro Kuwabara Yoshiyuki Fujii Yoshitaka |
| author_facet | Shinoda Noriyuki Sugiura Hironori Terashita Yukio Shibata Yasuyuki Ishiguro Hideyuki Haruki Nobuhiro Nishiwaki Tadashi Kudo Junzo Kimura Masahiro Kuwabara Yoshiyuki Fujii Yoshitaka |
| author_sort | Shinoda Noriyuki |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>β-catenin is a multifunctional protein involved in two apparently independent processes: cell-cell adhesion and signal transduction. β-catenin is involved in Wnt signaling pathway that regulates cellular differentiation and proliferation. In this study, we investigated the expression pattern of β-catenin and cyclin D1 using immunohistochemistry and searched for mutations in exon 3 of the β-catenin gene and Axin gene in esophageal squamous cell carcinoma.</p> <p>Materials and methods</p> <p>Samples were obtained from 50 esophageal cancer patients. Immunohistochemical staining for β-catenin and cyclin D1 was done. Mutational analyses of the exon3 of the β-catenin gene and Axin gene were performed on tumors with nuclear β-catenin expression.</p> <p>Results</p> <p>Four (8%) esophageal cancer tissues showed high nuclear β-catenin staining. Overexpression of cyclin D1 was observed in 27 out of 50 (54%) patients. All four cases that showed nuclear β-catenin staining overexpressed cyclin D1. No relationship was observed between the expression pattern of β-catenin and cyclin D1 and age, sex, tumor size, stage, differentiation grade, lymph node metastasis, response to chemotherapy, or survival. No mutational change was found in β-<it>catenin </it>exon 3 in the four cases with nuclear β-catenin staining. Sequencing analysis of the <it>Axin </it>cDNA revealed only a splicing variant (108 bp deletion, position 2302–2409) which was present in the paired normal mucosa.</p> <p>Conclusion</p> <p>A fraction of esophageal squamous cell carcinomas have abnormal nuclear accumulation of β-catenin accompanied with increased cyclin D1 expression. Mutations in β-catenin or axin genes are not responsible for this abnormal localization of β-catenin.</p> |
| first_indexed | 2024-04-14T08:14:19Z |
| format | Article |
| id | doaj.art-38fe267a0ed9440faa7b63c2453fedc4 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-14T08:14:19Z |
| publishDate | 2007-02-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-38fe267a0ed9440faa7b63c2453fedc42022-12-22T02:04:27ZengBMCWorld Journal of Surgical Oncology1477-78192007-02-01512110.1186/1477-7819-5-21Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancerShinoda NoriyukiSugiura HironoriTerashita YukioShibata YasuyukiIshiguro HideyukiHaruki NobuhiroNishiwaki TadashiKudo JunzoKimura MasahiroKuwabara YoshiyukiFujii Yoshitaka<p>Abstract</p> <p>Background</p> <p>β-catenin is a multifunctional protein involved in two apparently independent processes: cell-cell adhesion and signal transduction. β-catenin is involved in Wnt signaling pathway that regulates cellular differentiation and proliferation. In this study, we investigated the expression pattern of β-catenin and cyclin D1 using immunohistochemistry and searched for mutations in exon 3 of the β-catenin gene and Axin gene in esophageal squamous cell carcinoma.</p> <p>Materials and methods</p> <p>Samples were obtained from 50 esophageal cancer patients. Immunohistochemical staining for β-catenin and cyclin D1 was done. Mutational analyses of the exon3 of the β-catenin gene and Axin gene were performed on tumors with nuclear β-catenin expression.</p> <p>Results</p> <p>Four (8%) esophageal cancer tissues showed high nuclear β-catenin staining. Overexpression of cyclin D1 was observed in 27 out of 50 (54%) patients. All four cases that showed nuclear β-catenin staining overexpressed cyclin D1. No relationship was observed between the expression pattern of β-catenin and cyclin D1 and age, sex, tumor size, stage, differentiation grade, lymph node metastasis, response to chemotherapy, or survival. No mutational change was found in β-<it>catenin </it>exon 3 in the four cases with nuclear β-catenin staining. Sequencing analysis of the <it>Axin </it>cDNA revealed only a splicing variant (108 bp deletion, position 2302–2409) which was present in the paired normal mucosa.</p> <p>Conclusion</p> <p>A fraction of esophageal squamous cell carcinomas have abnormal nuclear accumulation of β-catenin accompanied with increased cyclin D1 expression. Mutations in β-catenin or axin genes are not responsible for this abnormal localization of β-catenin.</p>http://www.wjso.com/content/5/1/21 |
| spellingShingle | Shinoda Noriyuki Sugiura Hironori Terashita Yukio Shibata Yasuyuki Ishiguro Hideyuki Haruki Nobuhiro Nishiwaki Tadashi Kudo Junzo Kimura Masahiro Kuwabara Yoshiyuki Fujii Yoshitaka Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer World Journal of Surgical Oncology |
| title | Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer |
| title_full | Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer |
| title_fullStr | Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer |
| title_full_unstemmed | Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer |
| title_short | Aberrant nuclear localization of β-catenin without genetic alterations in β-catenin or Axin genes in esophageal cancer |
| title_sort | aberrant nuclear localization of β catenin without genetic alterations in β catenin or axin genes in esophageal cancer |
| url | http://www.wjso.com/content/5/1/21 |
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