Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells?
(1) Background: For advanced head and neck squamous cell carcinoma (HNSCC), boron neutron capture therapy (BNCT) is a potential therapeutic option, but high concentrations of boron within HNSCC are necessary. Therefore, this in vitro pilot study examined the uptake and concentration of boron in HNSC...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/16/7286 |
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| author | Matthias Gielisch Maximilian Moergel Bilal Al-Nawas Peer W. Kämmerer |
| author_facet | Matthias Gielisch Maximilian Moergel Bilal Al-Nawas Peer W. Kämmerer |
| author_sort | Matthias Gielisch |
| collection | DOAJ |
| description | (1) Background: For advanced head and neck squamous cell carcinoma (HNSCC), boron neutron capture therapy (BNCT) is a potential therapeutic option, but high concentrations of boron within HNSCC are necessary. Therefore, this in vitro pilot study examined the uptake and concentration of boron in HNSCC cells using the trans-stimulation effect of L-tyrosine when compared to non-stimulated samples. (2) Methods: Two HNSCC cell lines were incubated with L-tyrosine for up to two hours, followed by incubation with three L-para-boronophenylalanine (BPA) concentrations (5, 20, 50 ppm) at eight incubation times (1–4.5 h in half-hour steps). Subsequently, cellular boron uptake was measured via inductively coupled plasma mass spectrometry. (3) Results: No differences between laryngeal and oral SCC cells were seen; therefore, data were pooled. In total, boron uptake was not significantly increased in trans-stimulated samples when compared to the control group (all <i>p</i> > 0.05). Nevertheless, with trans-stimulation, higher BPA concentrations resulted in higher intracellular boron concentrations (5 < 20 < 50 ppm; all: <i>p</i> < 0.05), whereas these differences were less distinct in the non-trans-stimulated group. (4) Conclusions: The effect of trans-stimulation for up to two hours seems to be less relevant for HNSCC, though trans-stimulated HNSCC cells seem to have a more distinct BPA-dose-dependent cellular boron uptake that might be addressed in further research. |
| first_indexed | 2024-03-10T09:02:13Z |
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| id | doaj.art-38ff33936e9942b79543dc177c853b35 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T09:02:13Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-38ff33936e9942b79543dc177c853b352023-11-22T06:39:22ZengMDPI AGApplied Sciences2076-34172021-08-011116728610.3390/app11167286Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells?Matthias Gielisch0Maximilian Moergel1Bilal Al-Nawas2Peer W. Kämmerer3Department of Oral- and Maxillofacial Surgery, University Medical Center Mainz, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, 55131 Mainz, Germany(1) Background: For advanced head and neck squamous cell carcinoma (HNSCC), boron neutron capture therapy (BNCT) is a potential therapeutic option, but high concentrations of boron within HNSCC are necessary. Therefore, this in vitro pilot study examined the uptake and concentration of boron in HNSCC cells using the trans-stimulation effect of L-tyrosine when compared to non-stimulated samples. (2) Methods: Two HNSCC cell lines were incubated with L-tyrosine for up to two hours, followed by incubation with three L-para-boronophenylalanine (BPA) concentrations (5, 20, 50 ppm) at eight incubation times (1–4.5 h in half-hour steps). Subsequently, cellular boron uptake was measured via inductively coupled plasma mass spectrometry. (3) Results: No differences between laryngeal and oral SCC cells were seen; therefore, data were pooled. In total, boron uptake was not significantly increased in trans-stimulated samples when compared to the control group (all <i>p</i> > 0.05). Nevertheless, with trans-stimulation, higher BPA concentrations resulted in higher intracellular boron concentrations (5 < 20 < 50 ppm; all: <i>p</i> < 0.05), whereas these differences were less distinct in the non-trans-stimulated group. (4) Conclusions: The effect of trans-stimulation for up to two hours seems to be less relevant for HNSCC, though trans-stimulated HNSCC cells seem to have a more distinct BPA-dose-dependent cellular boron uptake that might be addressed in further research.https://www.mdpi.com/2076-3417/11/16/7286boron neutron capture therapytrans-stimulationhead and neck cancersquamous cell carcinomapreloading |
| spellingShingle | Matthias Gielisch Maximilian Moergel Bilal Al-Nawas Peer W. Kämmerer Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? Applied Sciences boron neutron capture therapy trans-stimulation head and neck cancer squamous cell carcinoma preloading |
| title | Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? |
| title_full | Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? |
| title_fullStr | Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? |
| title_full_unstemmed | Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? |
| title_short | Does Trans-Stimulation of L-Tyrosine Lead to an Increase in Boron Uptake in Head and Neck Squamous Cell Carcinoma Cells? |
| title_sort | does trans stimulation of l tyrosine lead to an increase in boron uptake in head and neck squamous cell carcinoma cells |
| topic | boron neutron capture therapy trans-stimulation head and neck cancer squamous cell carcinoma preloading |
| url | https://www.mdpi.com/2076-3417/11/16/7286 |
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