Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress
<i>Cordyceps chanhua</i> is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds of <i>C. chanhua</i>, has attracted much attention because of its medicinal value and food safety risk. In order to clear up the r...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/5/484 |
| _version_ | 1827668332573097984 |
|---|---|
| author | Cheng Zhao Haifen Bu Jiahua Zhu Yulong Wang Kerry M. Oliver Fenglin Hu Bo Huang Zengzhi Li Fan Peng |
| author_facet | Cheng Zhao Haifen Bu Jiahua Zhu Yulong Wang Kerry M. Oliver Fenglin Hu Bo Huang Zengzhi Li Fan Peng |
| author_sort | Cheng Zhao |
| collection | DOAJ |
| description | <i>Cordyceps chanhua</i> is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds of <i>C. chanhua</i>, has attracted much attention because of its medicinal value and food safety risk. In order to clear up the relationship between oxidative stress and BEA synthesis, we investigated the impact of H<sub>2</sub>O<sub>2</sub>-induced oxidative stress on the secondary metabolism of <i>C. chanhua</i> using untargeted metabolomics and a transcript profiling approach. Metabolic profiling of <i>C. chanhua</i> mycelia found that in total, 73 differential metabolites were identified, including organic acids, phospholipids, and non-ribosomal peptides (NRPs), especially the content of BEA, increasing 13-fold under oxidative stress treatment. Combining transcriptomic and metabolomic analyses, we found that the genes and metabolites associated with the NRP metabolism, especially the BEA biosynthesis, were highly significantly enriched under H<sub>2</sub>O<sub>2</sub>-induced stress, which indicated that the BEA metabolism might be positive in the resistance of <i>C. chanhua</i> to oxidative stress. These results not only aid in better understanding of the resistance mechanisms of <i>C. chanhua</i> against oxidative stress but also might be helpful for molecular breeding of <i>C. chanhua</i> with low BEA content. |
| first_indexed | 2024-03-10T03:37:25Z |
| format | Article |
| id | doaj.art-38ff9770ca4648f283a69e5995d80f39 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T03:37:25Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-38ff9770ca4648f283a69e5995d80f392023-11-23T11:41:46ZengMDPI AGJournal of Fungi2309-608X2022-05-018548410.3390/jof8050484Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative StressCheng Zhao0Haifen Bu1Jiahua Zhu2Yulong Wang3Kerry M. Oliver4Fenglin Hu5Bo Huang6Zengzhi Li7Fan Peng8Engineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Entomology, University of Georgia, Athens, GA 30602, USAEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, ChinaEngineering Research Center of Fungal Biotechnology, Ministry of Education, Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University, Hefei 230036, China<i>Cordyceps chanhua</i> is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds of <i>C. chanhua</i>, has attracted much attention because of its medicinal value and food safety risk. In order to clear up the relationship between oxidative stress and BEA synthesis, we investigated the impact of H<sub>2</sub>O<sub>2</sub>-induced oxidative stress on the secondary metabolism of <i>C. chanhua</i> using untargeted metabolomics and a transcript profiling approach. Metabolic profiling of <i>C. chanhua</i> mycelia found that in total, 73 differential metabolites were identified, including organic acids, phospholipids, and non-ribosomal peptides (NRPs), especially the content of BEA, increasing 13-fold under oxidative stress treatment. Combining transcriptomic and metabolomic analyses, we found that the genes and metabolites associated with the NRP metabolism, especially the BEA biosynthesis, were highly significantly enriched under H<sub>2</sub>O<sub>2</sub>-induced stress, which indicated that the BEA metabolism might be positive in the resistance of <i>C. chanhua</i> to oxidative stress. These results not only aid in better understanding of the resistance mechanisms of <i>C. chanhua</i> against oxidative stress but also might be helpful for molecular breeding of <i>C. chanhua</i> with low BEA content.https://www.mdpi.com/2309-608X/8/5/484<i>Cordyceps chanhua</i>beauvericinoxidative stressmetabolometranscriptome |
| spellingShingle | Cheng Zhao Haifen Bu Jiahua Zhu Yulong Wang Kerry M. Oliver Fenglin Hu Bo Huang Zengzhi Li Fan Peng Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress Journal of Fungi <i>Cordyceps chanhua</i> beauvericin oxidative stress metabolome transcriptome |
| title | Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_full | Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_fullStr | Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_full_unstemmed | Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_short | Integration of Untargeted Metabolomics with Transcriptomics Provides Insights into Beauvericin Biosynthesis in <i>Cordyceps chanhua</i> under H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_sort | integration of untargeted metabolomics with transcriptomics provides insights into beauvericin biosynthesis in i cordyceps chanhua i under h sub 2 sub o sub 2 sub induced oxidative stress |
| topic | <i>Cordyceps chanhua</i> beauvericin oxidative stress metabolome transcriptome |
| url | https://www.mdpi.com/2309-608X/8/5/484 |
| work_keys_str_mv | AT chengzhao integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT haifenbu integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT jiahuazhu integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT yulongwang integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT kerrymoliver integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT fenglinhu integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT bohuang integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT zengzhili integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress AT fanpeng integrationofuntargetedmetabolomicswithtranscriptomicsprovidesinsightsintobeauvericinbiosynthesisinicordycepschanhuaiunderhsub2subosub2subinducedoxidativestress |