Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion
Objective (s) Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed t...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2011-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a3.pdf |
| _version_ | 1818394493614817280 |
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| author | Mohammad Ali Atlasi Jose Luis Perez Velazquez |
| author_facet | Mohammad Ali Atlasi Jose Luis Perez Velazquez |
| author_sort | Mohammad Ali Atlasi |
| collection | DOAJ |
| description | Objective (s) Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed the hypothesis that porin interacts with other mitochondrial proteins after ischemic injury.Materials and MethodsFor this purpose, we used in vivo 4-vessel occlusion model of rat brain and porin purification method by hydroxyapatite column. After SDS gel electrophoresis and silver nitrate staining, Western blotting was done for porin, adenine nucleotide translocase and cyclophilin-D proteins.Results Porin was purified from mitochondrial mixture in ischemic brain and control groups. Investigation of interaction of adenine nucleotide transposes (ANT) and cyclophilin-D with porin by Western blotting showed no proteins co-purified with porin from injured tissues.Conclusion The present study implies that there may not be interaction between porin, and ANT or cyclophilin-D, and if there is any, it is not maintained during the purification procedure. |
| first_indexed | 2024-12-14T06:02:05Z |
| format | Article |
| id | doaj.art-38ffc433fb394c6c8951f817df78a8e1 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-14T06:02:05Z |
| publishDate | 2011-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-38ffc433fb394c6c8951f817df78a8e12022-12-21T23:14:23ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742011-01-011448Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and ReperfusionMohammad Ali AtlasiJose Luis Perez VelazquezObjective (s) Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed the hypothesis that porin interacts with other mitochondrial proteins after ischemic injury.Materials and MethodsFor this purpose, we used in vivo 4-vessel occlusion model of rat brain and porin purification method by hydroxyapatite column. After SDS gel electrophoresis and silver nitrate staining, Western blotting was done for porin, adenine nucleotide translocase and cyclophilin-D proteins.Results Porin was purified from mitochondrial mixture in ischemic brain and control groups. Investigation of interaction of adenine nucleotide transposes (ANT) and cyclophilin-D with porin by Western blotting showed no proteins co-purified with porin from injured tissues.Conclusion The present study implies that there may not be interaction between porin, and ANT or cyclophilin-D, and if there is any, it is not maintained during the purification procedure.http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a3.pdfIschemiaMitochondrial permeability transition porePorinsReperfusion |
| spellingShingle | Mohammad Ali Atlasi Jose Luis Perez Velazquez Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion Iranian Journal of Basic Medical Sciences Ischemia Mitochondrial permeability transition pore Porins Reperfusion |
| title | Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion |
| title_full | Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion |
| title_fullStr | Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion |
| title_full_unstemmed | Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion |
| title_short | Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion |
| title_sort | evaluation of porin interaction with adenine nucleotide translocase and cyclophilin d proteins after brain ischemia and reperfusion |
| topic | Ischemia Mitochondrial permeability transition pore Porins Reperfusion |
| url | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a3.pdf |
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