The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress
Abstract Objective This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiation-induced male fertility impairment. Methods The characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evalu...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2022-05-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40824-022-00266-6 |
| _version_ | 1827333416322859008 |
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| author | Jing Ma Suhe Dong Hongtao Lu Zhongmin Chen Huijie Yu Xuejun Sun Renjun Peng Wei Li Sinian Wang Qisheng Jiang Fengsheng Li Li Ma |
| author_facet | Jing Ma Suhe Dong Hongtao Lu Zhongmin Chen Huijie Yu Xuejun Sun Renjun Peng Wei Li Sinian Wang Qisheng Jiang Fengsheng Li Li Ma |
| author_sort | Jing Ma |
| collection | DOAJ |
| description | Abstract Objective This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiation-induced male fertility impairment. Methods The characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evaluated in vivo and in vitro. The radioprotective effect of MgH2 on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms. Results MgH2 had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH2 was safe and nontoxic in mice and cells. After irradiation, MgH2 treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH2 treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH2 eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH2 significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH2 inhibited the activation of radiation-induced inflammation and pyroptosis. Conclusion MgH2 improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals. Graphical abstract Mice fertility and function were evaluated with or without MgH2 treatment after 5 Gy irradiation. MgH2 had the ability of hydroxyl radicals scavenging and MDA suppressing in testicular tissue induced by irradiation. Further, MgH2 could participate in spermatogenesis and protect sperm development in three stages: the generation of Sertoli cells (Sox-9+), spermatogonia (Stra8+) and round sperm cells (Crem+). Moreover, MgH2 alleviated the decrease of testosterone secreted by interstitial cells after irradiation. In addition, MgH2 suppressed apoptosis, pyroptosis and inflammatory response and alleviated cell cycle arrest by mediating IR-induced ROS. |
| first_indexed | 2024-03-07T17:25:04Z |
| format | Article |
| id | doaj.art-39000f8795ac483bb4f1bf3df4efdbfa |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T17:25:04Z |
| publishDate | 2022-05-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-39000f8795ac483bb4f1bf3df4efdbfa2024-03-02T19:18:36ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242022-05-0126111510.1186/s40824-022-00266-6The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stressJing Ma0Suhe Dong1Hongtao Lu2Zhongmin Chen3Huijie Yu4Xuejun Sun5Renjun Peng6Wei Li7Sinian Wang8Qisheng Jiang9Fengsheng Li10Li Ma11The Postgraduate Training Base of Jinzhou Medical University (The PLA Rocket Force Characteristic Medical Center)PLA Rocket Force Characteristic Medical CenterDepartment of Naval Medicine, Naval Medical UniversityPLA Rocket Force Characteristic Medical CenterPLA Rocket Force Characteristic Medical CenterDepartment of Naval Medicine, Naval Medical UniversityPLA Rocket Force Characteristic Medical CenterPLA Rocket Force Characteristic Medical CenterPLA Rocket Force Characteristic Medical CenterPLA Rocket Force Characteristic Medical CenterPLA Rocket Force Characteristic Medical CenterThe Postgraduate Training Base of Jinzhou Medical University (The PLA Rocket Force Characteristic Medical Center)Abstract Objective This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiation-induced male fertility impairment. Methods The characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evaluated in vivo and in vitro. The radioprotective effect of MgH2 on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms. Results MgH2 had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH2 was safe and nontoxic in mice and cells. After irradiation, MgH2 treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH2 treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH2 eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH2 significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH2 inhibited the activation of radiation-induced inflammation and pyroptosis. Conclusion MgH2 improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals. Graphical abstract Mice fertility and function were evaluated with or without MgH2 treatment after 5 Gy irradiation. MgH2 had the ability of hydroxyl radicals scavenging and MDA suppressing in testicular tissue induced by irradiation. Further, MgH2 could participate in spermatogenesis and protect sperm development in three stages: the generation of Sertoli cells (Sox-9+), spermatogonia (Stra8+) and round sperm cells (Crem+). Moreover, MgH2 alleviated the decrease of testosterone secreted by interstitial cells after irradiation. In addition, MgH2 suppressed apoptosis, pyroptosis and inflammatory response and alleviated cell cycle arrest by mediating IR-induced ROS.https://doi.org/10.1186/s40824-022-00266-6NanomaterialIrradiation damageMale infertilityROSHydroxyl radical |
| spellingShingle | Jing Ma Suhe Dong Hongtao Lu Zhongmin Chen Huijie Yu Xuejun Sun Renjun Peng Wei Li Sinian Wang Qisheng Jiang Fengsheng Li Li Ma The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress Biomaterials Research Nanomaterial Irradiation damage Male infertility ROS Hydroxyl radical |
| title | The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress |
| title_full | The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress |
| title_fullStr | The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress |
| title_full_unstemmed | The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress |
| title_short | The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress |
| title_sort | hydrogen storage nanomaterial mgh2 improves irradiation induced male fertility impairment by suppressing oxidative stress |
| topic | Nanomaterial Irradiation damage Male infertility ROS Hydroxyl radical |
| url | https://doi.org/10.1186/s40824-022-00266-6 |
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