A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt
Background: Proteus mirabilias ( P. mirabilis) strains are motile Gram negativerods that colonize the humangastrointestinal tract and could be a source of nosocomial infections especially in immune compromised cancer patients. Proteus mirabilis is identified by its urease production as a major bact...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zagazig University, Faculty of Medicine
2022-10-01
|
| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_256743_d3c4fd0eeb4db2d9aad410213640dcc0.pdf |
| _version_ | 1797989226241851392 |
|---|---|
| author | Sawsan Hanem El Tayeb Mohamed Abdelhamed May SOLIMAN Amani El Kholy Doaa Gamal |
| author_facet | Sawsan Hanem El Tayeb Mohamed Abdelhamed May SOLIMAN Amani El Kholy Doaa Gamal |
| author_sort | Sawsan Hanem El Tayeb |
| collection | DOAJ |
| description | Background: Proteus mirabilias ( P. mirabilis) strains are motile Gram negativerods that colonize the humangastrointestinal tract and could be a source of nosocomial infections especially in immune compromised cancer patients. Proteus mirabilis is identified by its urease production as a major bacterial virulence especially in urinary tract infections. Aim: This study aimed to characterize a case of a urease negative P. mirabilis isolated from a cancer patient suffering from deep surgical site infection. Methods: Conventional biochemical tests performed on swarming bacterial growth cultures were characteristic of P. mirabilis except for urease production. Identification was confirmed by Vitek-2 compact system and Vitek- MS. Dienes test proved the isolate is antigenically different from other urease positive P. mirabilis. Whole genome sequencing (WGS) identified this P. mirabilis strain with the seven encoding urease genes ureABCDEFG as well as their positive regulator gene UreR. Results: Mutations were found within amino acid sequences of UreB, UreE, UreF and UreG and UreR. Antibiotic sensitivity as well as WGS identified resistance genes to multiple classes of antibiotics. Conclusion: This is the first report of a wildly existed urease negative P. mirabilis due to mutations in the urease gene cluster (UreABC) as well as the regulator UreR. Questions are raised towards the urease enzyme as a key virulence factor to P. mirabilis. Other virulence factors that allow this species to cause other severe infections should be considered. The rate of isolation of urease negative P. mirabilis should be monitored and assessed in the future. |
| first_indexed | 2024-04-11T08:16:44Z |
| format | Article |
| id | doaj.art-3901b27f4c9a447d960c0c467f8e1e4a |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-11T08:16:44Z |
| publishDate | 2022-10-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-3901b27f4c9a447d960c0c467f8e1e4a2022-12-22T04:35:06ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402022-10-013497297810.21608/mid.2022.153180.1360256743A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, EgyptSawsan Hanem El Tayeb0Mohamed Abdelhamed1May SOLIMAN2Amani El Kholy3Doaa Gamal4Clinical microbiology,Medical college Al Azhar universityclinical pathology, Al Azhar universityClinical pathology department , Cairo universityClinical pathology, Kasr Ainy school of medicine, Cairo universityMicrobiology Department, Theodor Bilharz Research instituteBackground: Proteus mirabilias ( P. mirabilis) strains are motile Gram negativerods that colonize the humangastrointestinal tract and could be a source of nosocomial infections especially in immune compromised cancer patients. Proteus mirabilis is identified by its urease production as a major bacterial virulence especially in urinary tract infections. Aim: This study aimed to characterize a case of a urease negative P. mirabilis isolated from a cancer patient suffering from deep surgical site infection. Methods: Conventional biochemical tests performed on swarming bacterial growth cultures were characteristic of P. mirabilis except for urease production. Identification was confirmed by Vitek-2 compact system and Vitek- MS. Dienes test proved the isolate is antigenically different from other urease positive P. mirabilis. Whole genome sequencing (WGS) identified this P. mirabilis strain with the seven encoding urease genes ureABCDEFG as well as their positive regulator gene UreR. Results: Mutations were found within amino acid sequences of UreB, UreE, UreF and UreG and UreR. Antibiotic sensitivity as well as WGS identified resistance genes to multiple classes of antibiotics. Conclusion: This is the first report of a wildly existed urease negative P. mirabilis due to mutations in the urease gene cluster (UreABC) as well as the regulator UreR. Questions are raised towards the urease enzyme as a key virulence factor to P. mirabilis. Other virulence factors that allow this species to cause other severe infections should be considered. The rate of isolation of urease negative P. mirabilis should be monitored and assessed in the future.https://mid.journals.ekb.eg/article_256743_d3c4fd0eeb4db2d9aad410213640dcc0.pdfp. mirabilisureaseureabcsurgical skin infectionwgs |
| spellingShingle | Sawsan Hanem El Tayeb Mohamed Abdelhamed May SOLIMAN Amani El Kholy Doaa Gamal A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt Microbes and Infectious Diseases p. mirabilis urease ureabc surgical skin infection wgs |
| title | A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt |
| title_full | A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt |
| title_fullStr | A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt |
| title_full_unstemmed | A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt |
| title_short | A case oriented study: Whole genome sequencing (WGS) on a wild urease negative Proteus mirabilis isolated from deep surgical site infection (SSI) at El Hussein University Hospital, Al Azhar University Cairo, Egypt |
| title_sort | case oriented study whole genome sequencing wgs on a wild urease negative proteus mirabilis isolated from deep surgical site infection ssi at el hussein university hospital al azhar university cairo egypt |
| topic | p. mirabilis urease ureabc surgical skin infection wgs |
| url | https://mid.journals.ekb.eg/article_256743_d3c4fd0eeb4db2d9aad410213640dcc0.pdf |
| work_keys_str_mv | AT sawsanhanemeltayeb acaseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT mohamedabdelhamed acaseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT maysoliman acaseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT amanielkholy acaseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT doaagamal acaseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT sawsanhanemeltayeb caseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT mohamedabdelhamed caseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT maysoliman caseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT amanielkholy caseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt AT doaagamal caseorientedstudywholegenomesequencingwgsonawildureasenegativeproteusmirabilisisolatedfromdeepsurgicalsiteinfectionssiatelhusseinuniversityhospitalalazharuniversitycairoegypt |