Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods
Microplastic (MP) contamination is a globally recognised issue in aquatic environments, and recently, there has been an increase in investigations focusing on lake contamination, revealing significant amounts of dispersed MPs. However, our understanding of the ingestion and effects of MPs on organis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Environments |
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| Online Access: | https://www.mdpi.com/2076-3298/10/7/115 |
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| author | Camilla Ciotti Andrea Setini Francesca Lecce Valentina Iannilli |
| author_facet | Camilla Ciotti Andrea Setini Francesca Lecce Valentina Iannilli |
| author_sort | Camilla Ciotti |
| collection | DOAJ |
| description | Microplastic (MP) contamination is a globally recognised issue in aquatic environments, and recently, there has been an increase in investigations focusing on lake contamination, revealing significant amounts of dispersed MPs. However, our understanding of the ingestion and effects of MPs on organisms living in lake ecosystems remains limited. This study aims to develop an effective protocol for assessing the ingestion of MPs by the talitrid amphipod <i>Cryptorchestia garbinii</i>, with the goal of verifying and evaluating the biological effects following ingestion. Individuals sampled from the shores of Lake Albano were exposed to four different polymers, namely low-density polyethylene (PE), polyethylene terephthalate (PET), polyester (PES), and polypropylene (PP), under laboratory conditions. To deliver MPs through the diet, we decided to employ DECOTABs (DEcomposition and COnsumption TABlets) which have been successfully used as a food source in aquatic toxicity tests. At the end of the experiments, we employed the solvatochromic and fluorescent dye Nile red to detect and quantify the MPs present in the digestive tube contents of the animals. The results clearly demonstrate the ingestion of the supplied polymers through the tabs, validating this method of exposure as effective. Furthermore, the measurement of glucose, glycogen, and lipid levels reveals that within 24 h of ingestion, MPs had an impact on the macromolecules involved in the energy metabolism of <i>C. garbinii</i>. This research underscores the suitability of this species as a model organism for studying MP uptake and its effects. |
| first_indexed | 2024-03-11T01:05:31Z |
| format | Article |
| id | doaj.art-3902d942d94b4e3cb91dd4e549a22624 |
| institution | Directory Open Access Journal |
| issn | 2076-3298 |
| language | English |
| last_indexed | 2024-03-11T01:05:31Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
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| series | Environments |
| spelling | doaj.art-3902d942d94b4e3cb91dd4e549a226242023-11-18T19:15:08ZengMDPI AGEnvironments2076-32982023-07-0110711510.3390/environments10070115Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid AmphipodsCamilla Ciotti0Andrea Setini1Francesca Lecce2Valentina Iannilli3ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, C.R. Casaccia, Via Anguillarese 301, 00123 Rome, ItalyDepartment of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, C.R. Casaccia, Via Anguillarese 301, 00123 Rome, ItalyENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, C.R. Casaccia, Via Anguillarese 301, 00123 Rome, ItalyMicroplastic (MP) contamination is a globally recognised issue in aquatic environments, and recently, there has been an increase in investigations focusing on lake contamination, revealing significant amounts of dispersed MPs. However, our understanding of the ingestion and effects of MPs on organisms living in lake ecosystems remains limited. This study aims to develop an effective protocol for assessing the ingestion of MPs by the talitrid amphipod <i>Cryptorchestia garbinii</i>, with the goal of verifying and evaluating the biological effects following ingestion. Individuals sampled from the shores of Lake Albano were exposed to four different polymers, namely low-density polyethylene (PE), polyethylene terephthalate (PET), polyester (PES), and polypropylene (PP), under laboratory conditions. To deliver MPs through the diet, we decided to employ DECOTABs (DEcomposition and COnsumption TABlets) which have been successfully used as a food source in aquatic toxicity tests. At the end of the experiments, we employed the solvatochromic and fluorescent dye Nile red to detect and quantify the MPs present in the digestive tube contents of the animals. The results clearly demonstrate the ingestion of the supplied polymers through the tabs, validating this method of exposure as effective. Furthermore, the measurement of glucose, glycogen, and lipid levels reveals that within 24 h of ingestion, MPs had an impact on the macromolecules involved in the energy metabolism of <i>C. garbinii</i>. This research underscores the suitability of this species as a model organism for studying MP uptake and its effects.https://www.mdpi.com/2076-3298/10/7/115Nile redmicroplastic trophic transferDECOTAB<i>Cryptorchestia</i>energy reserves |
| spellingShingle | Camilla Ciotti Andrea Setini Francesca Lecce Valentina Iannilli Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods Environments Nile red microplastic trophic transfer DECOTAB <i>Cryptorchestia</i> energy reserves |
| title | Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods |
| title_full | Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods |
| title_fullStr | Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods |
| title_full_unstemmed | Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods |
| title_short | Uncovering the Hidden Dangers of Microplastic Pollution in Lake Ecosystems: Effects of Ingestion on Talitrid Amphipods |
| title_sort | uncovering the hidden dangers of microplastic pollution in lake ecosystems effects of ingestion on talitrid amphipods |
| topic | Nile red microplastic trophic transfer DECOTAB <i>Cryptorchestia</i> energy reserves |
| url | https://www.mdpi.com/2076-3298/10/7/115 |
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