Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines
Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuatio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.676582/full |
| _version_ | 1818653300823687168 |
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| author | Marianoel Pereira-Gómez Marianoel Pereira-Gómez Lucía Carrau Álvaro Fajardo Álvaro Fajardo Pilar Moreno Pilar Moreno Gonzalo Moratorio Gonzalo Moratorio |
| author_facet | Marianoel Pereira-Gómez Marianoel Pereira-Gómez Lucía Carrau Álvaro Fajardo Álvaro Fajardo Pilar Moreno Pilar Moreno Gonzalo Moratorio Gonzalo Moratorio |
| author_sort | Marianoel Pereira-Gómez |
| collection | DOAJ |
| description | Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines. |
| first_indexed | 2024-12-17T02:35:43Z |
| format | Article |
| id | doaj.art-39040cd9277647b7b2f9368726f5e494 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T02:35:43Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-39040cd9277647b7b2f9368726f5e4942022-12-21T22:06:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.676582676582Altering Compositional Properties of Viral Genomes to Design Live-Attenuated VaccinesMarianoel Pereira-Gómez0Marianoel Pereira-Gómez1Lucía Carrau2Álvaro Fajardo3Álvaro Fajardo4Pilar Moreno5Pilar Moreno6Gonzalo Moratorio7Gonzalo Moratorio8Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayLaboratorio de Evolución Experimental de Virus, Institut Pasteur de Montevideo, Montevideo, UruguayDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United StatesLaboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayLaboratorio de Evolución Experimental de Virus, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayLaboratorio de Evolución Experimental de Virus, Institut Pasteur de Montevideo, Montevideo, UruguayLaboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, UruguayLaboratorio de Evolución Experimental de Virus, Institut Pasteur de Montevideo, Montevideo, UruguayLive-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines.https://www.frontiersin.org/articles/10.3389/fmicb.2021.676582/fullRNA virusesgenome compositioncodon usagecodon pair biasmutational robustnessattenuation |
| spellingShingle | Marianoel Pereira-Gómez Marianoel Pereira-Gómez Lucía Carrau Álvaro Fajardo Álvaro Fajardo Pilar Moreno Pilar Moreno Gonzalo Moratorio Gonzalo Moratorio Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines Frontiers in Microbiology RNA viruses genome composition codon usage codon pair bias mutational robustness attenuation |
| title | Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines |
| title_full | Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines |
| title_fullStr | Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines |
| title_full_unstemmed | Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines |
| title_short | Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines |
| title_sort | altering compositional properties of viral genomes to design live attenuated vaccines |
| topic | RNA viruses genome composition codon usage codon pair bias mutational robustness attenuation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.676582/full |
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