α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling
Oral submucous fibrosis (OSF) is a premalignant disorder and persistent activation of myofibroblasts is implicated in this pathological progression. Increasing attention has been addressed towards non-coding RNA-regulated myofibroblasts activities and the effects of phytochemicals on non-coding RNA...
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MDPI AG
2023-03-01
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author | Yu-Hsien Lee Pei-Ling Hsieh Shih-Chi Chao Yi-Wen Liao Chia-Ming Liu Cheng-Chia Yu |
author_facet | Yu-Hsien Lee Pei-Ling Hsieh Shih-Chi Chao Yi-Wen Liao Chia-Ming Liu Cheng-Chia Yu |
author_sort | Yu-Hsien Lee |
collection | DOAJ |
description | Oral submucous fibrosis (OSF) is a premalignant disorder and persistent activation of myofibroblasts is implicated in this pathological progression. Increasing attention has been addressed towards non-coding RNA-regulated myofibroblasts activities and the effects of phytochemicals on non-coding RNA modulation are of great importance. In the present study, we examined the anti-fibrosis property of α-mangostin, a xanthone isolated from the pericarp of mangosteen. We found that α-mangostin exhibited inhibitory potency in myofibroblast activities and expression of fibrosis markers at the concentrations that caused neglectable damage to normal cells. Apart from the downregulation of TGF-β1/Smad2 signaling, we found that α-mangostin attenuated the expression of long non-coding RNA LincROR as well. Our results demonstrated that the effects of α-mangostin on myofibroblast activation were reverted when LincROR was overexpressed. Additionally, we showed the expression of LincROR in OSF specimens was elevated and silencing of LincROR successfully attenuated myofibroblast characteristics and TGF-β1/Smad2 activation. Taken together, these findings indicated that the anti-fibrosis effects of α-mangostin merit consideration and may be due to the attenuation of LincROR. |
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language | English |
last_indexed | 2024-03-11T06:04:58Z |
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spelling | doaj.art-390439853272489e87a832a572bd1bad2023-11-17T13:04:09ZengMDPI AGNutrients2072-66432023-03-01156132110.3390/nu15061321α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad SignalingYu-Hsien Lee0Pei-Ling Hsieh1Shih-Chi Chao2Yi-Wen Liao3Chia-Ming Liu4Cheng-Chia Yu5School of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung 404333, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung 40201, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung 40201, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanOral submucous fibrosis (OSF) is a premalignant disorder and persistent activation of myofibroblasts is implicated in this pathological progression. Increasing attention has been addressed towards non-coding RNA-regulated myofibroblasts activities and the effects of phytochemicals on non-coding RNA modulation are of great importance. In the present study, we examined the anti-fibrosis property of α-mangostin, a xanthone isolated from the pericarp of mangosteen. We found that α-mangostin exhibited inhibitory potency in myofibroblast activities and expression of fibrosis markers at the concentrations that caused neglectable damage to normal cells. Apart from the downregulation of TGF-β1/Smad2 signaling, we found that α-mangostin attenuated the expression of long non-coding RNA LincROR as well. Our results demonstrated that the effects of α-mangostin on myofibroblast activation were reverted when LincROR was overexpressed. Additionally, we showed the expression of LincROR in OSF specimens was elevated and silencing of LincROR successfully attenuated myofibroblast characteristics and TGF-β1/Smad2 activation. Taken together, these findings indicated that the anti-fibrosis effects of α-mangostin merit consideration and may be due to the attenuation of LincROR.https://www.mdpi.com/2072-6643/15/6/1321oral submucous fibrosismyofibroblastα-mangostinLincROR |
spellingShingle | Yu-Hsien Lee Pei-Ling Hsieh Shih-Chi Chao Yi-Wen Liao Chia-Ming Liu Cheng-Chia Yu α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling Nutrients oral submucous fibrosis myofibroblast α-mangostin LincROR |
title | α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling |
title_full | α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling |
title_fullStr | α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling |
title_full_unstemmed | α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling |
title_short | α-Mangostin Inhibits the Activation of Myofibroblasts via Downregulation of Linc-ROR-Mediated TGFB1/Smad Signaling |
title_sort | α mangostin inhibits the activation of myofibroblasts via downregulation of linc ror mediated tgfb1 smad signaling |
topic | oral submucous fibrosis myofibroblast α-mangostin LincROR |
url | https://www.mdpi.com/2072-6643/15/6/1321 |
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