The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source

This present study determined antioxidant activity, lipid peroxidation, total phenolic, total flavonoids and phytochemicals in moringa leaves and moringa stem. Analysis used in this study was 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method for antioxidant activity, thiobarbituric acid reactive substanc...

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Main Authors: A. Fitri, T. Toharmat, D. A. Astuti, H. Tamura
Format: Article
Language:English
Published: Bogor Agricultural University 2015-12-01
Series:Media Peternakan
Subjects:
Online Access:http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/9612
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author A. Fitri
T. Toharmat
D. A. Astuti
H. Tamura
author_facet A. Fitri
T. Toharmat
D. A. Astuti
H. Tamura
author_sort A. Fitri
collection DOAJ
description This present study determined antioxidant activity, lipid peroxidation, total phenolic, total flavonoids and phytochemicals in moringa leaves and moringa stem. Analysis used in this study was 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method for antioxidant activity, thiobarbituric acid reactive substances (TBARS) method for lipid peroxidation, Folin-Ciocalteu method for total phenolic, total flavonoid and UFLC (Ultrafast Liquid Chromatography) for identification and quantification of phenolic compounds. The results showed that moringa leaves had higher ability to scavenge free radical, total phenolic, and total flavonoid than moringa stem (P<0.001). Malondialdehyde production, the end product of lipid peroxidation, in moringa leaves was lower than moringa stem (P<0.001). Ferulic acid was the major active compound in both moringa leaves and moringa stem. This present study indicated that moringa leaves and moringa stem could be used as feed additive which had a good potential to prevent oxidative stress in animals.
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spelling doaj.art-390561403cb0405cbab2aa506b9a83d62022-12-22T02:01:06ZengBogor Agricultural UniversityMedia Peternakan0126-04722087-46342015-12-0138316917510.5398/medpet.2015.38.3.169The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant SourceA. Fitri 0T. Toharmat 1D. A. Astuti 2H. Tamura3Study Program of Nutrition and Feed Science, Faculty of Animal Science, Graduate School, Bogor Agricultural University, IndonesiaDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, JapanThis present study determined antioxidant activity, lipid peroxidation, total phenolic, total flavonoids and phytochemicals in moringa leaves and moringa stem. Analysis used in this study was 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method for antioxidant activity, thiobarbituric acid reactive substances (TBARS) method for lipid peroxidation, Folin-Ciocalteu method for total phenolic, total flavonoid and UFLC (Ultrafast Liquid Chromatography) for identification and quantification of phenolic compounds. The results showed that moringa leaves had higher ability to scavenge free radical, total phenolic, and total flavonoid than moringa stem (P<0.001). Malondialdehyde production, the end product of lipid peroxidation, in moringa leaves was lower than moringa stem (P<0.001). Ferulic acid was the major active compound in both moringa leaves and moringa stem. This present study indicated that moringa leaves and moringa stem could be used as feed additive which had a good potential to prevent oxidative stress in animals.http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/9612antioxidantlipid peroxidationmoringa leavesmoringa stemphytochemicals
spellingShingle A. Fitri
T. Toharmat
D. A. Astuti
H. Tamura
The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
Media Peternakan
antioxidant
lipid peroxidation
moringa leaves
moringa stem
phytochemicals
title The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
title_full The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
title_fullStr The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
title_full_unstemmed The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
title_short The Potential Use of Secondary Metabolites in Moringa oleifera as an Antioxidant Source
title_sort potential use of secondary metabolites in moringa oleifera as an antioxidant source
topic antioxidant
lipid peroxidation
moringa leaves
moringa stem
phytochemicals
url http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/9612
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