Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.)
Drought tolerance is a complex trait controlled by many metabolic pathways and genes and identifying a solution to increase the resilience of plants to drought stress is one of the grand challenges in plant biology. This study provided compelling evidence of increased drought stress tolerance in two...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741020/?tool=EBI |
| _version_ | 1819228835809329152 |
|---|---|
| author | Md Jahirul Islam Md Jalal Uddin Mohammad Anwar Hossain Robert Henry Mst. Kohinoor Begum Md. Abu Taher Sohel Masuma Akter Mou Juhee Ahn Eun Ju Cheong Young-Seok Lim |
| author_facet | Md Jahirul Islam Md Jalal Uddin Mohammad Anwar Hossain Robert Henry Mst. Kohinoor Begum Md. Abu Taher Sohel Masuma Akter Mou Juhee Ahn Eun Ju Cheong Young-Seok Lim |
| author_sort | Md Jahirul Islam |
| collection | DOAJ |
| description | Drought tolerance is a complex trait controlled by many metabolic pathways and genes and identifying a solution to increase the resilience of plants to drought stress is one of the grand challenges in plant biology. This study provided compelling evidence of increased drought stress tolerance in two sugar beet genotypes when treated with exogenous putrescine (Put) at the seedling stage. Morpho-physiological and biochemical traits and gene expression were assessed in thirty-day-old sugar beet seedlings subjected to drought stress with or without Put (0.3, 0.6, and 0.9 mM) application. Sugar beet plants exposed to drought stress exhibited a significant decline in growth and development as evidenced by root and shoot growth characteristics, photosynthetic pigments, antioxidant enzyme activities, and gene expression. Drought stress resulted in a sharp increase in hydrogen peroxide (H2O2) (89.4 and 118% in SBT-010 and BSRI Sugar beet 2, respectively) and malondialdehyde (MDA) (35.6 and 27.1% in SBT-010 and BSRI Sugar beet 2, respectively). These changes were strongly linked to growth retardation as evidenced by principal component analysis (PCA) and heatmap clustering. Importantly, Put-sprayed plants suffered from less oxidative stress as indicated by lower H2O2 and MDA accumulation. They better regulated the physiological processes supporting growth, dry matter accumulation, photosynthetic pigmentation and gas exchange, relative water content; modulated biochemical changes including proline, total soluble carbohydrate, total soluble sugar, and ascorbic acid; and enhanced the activities of antioxidant enzymes and gene expression. PCA results strongly suggested that Put conferred drought tolerance mostly by enhancing antioxidant enzymes activities that regulated homeostasis of reactive oxygen species. These findings collectively provide an important illustration of the use of Put in modulating drought tolerance in sugar beet plants. |
| first_indexed | 2024-12-23T11:03:36Z |
| format | Article |
| id | doaj.art-3905c4ce7fe04155894a8c6beec14420 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:03:36Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3905c4ce7fe04155894a8c6beec144202022-12-21T17:49:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01171Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.)Md Jahirul IslamMd Jalal UddinMohammad Anwar HossainRobert HenryMst. Kohinoor BegumMd. Abu Taher SohelMasuma Akter MouJuhee AhnEun Ju CheongYoung-Seok LimDrought tolerance is a complex trait controlled by many metabolic pathways and genes and identifying a solution to increase the resilience of plants to drought stress is one of the grand challenges in plant biology. This study provided compelling evidence of increased drought stress tolerance in two sugar beet genotypes when treated with exogenous putrescine (Put) at the seedling stage. Morpho-physiological and biochemical traits and gene expression were assessed in thirty-day-old sugar beet seedlings subjected to drought stress with or without Put (0.3, 0.6, and 0.9 mM) application. Sugar beet plants exposed to drought stress exhibited a significant decline in growth and development as evidenced by root and shoot growth characteristics, photosynthetic pigments, antioxidant enzyme activities, and gene expression. Drought stress resulted in a sharp increase in hydrogen peroxide (H2O2) (89.4 and 118% in SBT-010 and BSRI Sugar beet 2, respectively) and malondialdehyde (MDA) (35.6 and 27.1% in SBT-010 and BSRI Sugar beet 2, respectively). These changes were strongly linked to growth retardation as evidenced by principal component analysis (PCA) and heatmap clustering. Importantly, Put-sprayed plants suffered from less oxidative stress as indicated by lower H2O2 and MDA accumulation. They better regulated the physiological processes supporting growth, dry matter accumulation, photosynthetic pigmentation and gas exchange, relative water content; modulated biochemical changes including proline, total soluble carbohydrate, total soluble sugar, and ascorbic acid; and enhanced the activities of antioxidant enzymes and gene expression. PCA results strongly suggested that Put conferred drought tolerance mostly by enhancing antioxidant enzymes activities that regulated homeostasis of reactive oxygen species. These findings collectively provide an important illustration of the use of Put in modulating drought tolerance in sugar beet plants.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741020/?tool=EBI |
| spellingShingle | Md Jahirul Islam Md Jalal Uddin Mohammad Anwar Hossain Robert Henry Mst. Kohinoor Begum Md. Abu Taher Sohel Masuma Akter Mou Juhee Ahn Eun Ju Cheong Young-Seok Lim Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) PLoS ONE |
| title | Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) |
| title_full | Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) |
| title_fullStr | Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) |
| title_full_unstemmed | Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) |
| title_short | Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.) |
| title_sort | exogenous putrescine attenuates the negative impact of drought stress by modulating physio biochemical traits and gene expression in sugar beet beta vulgaris l |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741020/?tool=EBI |
| work_keys_str_mv | AT mdjahirulislam exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT mdjalaluddin exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT mohammadanwarhossain exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT roberthenry exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT mstkohinoorbegum exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT mdabutahersohel exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT masumaaktermou exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT juheeahn exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT eunjucheong exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl AT youngseoklim exogenousputrescineattenuatesthenegativeimpactofdroughtstressbymodulatingphysiobiochemicaltraitsandgeneexpressioninsugarbeetbetavulgarisl |