Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
Time-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of micr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.681202/full |
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| author | Hongyu Wang Hongyu Wang Pengke Xia Pengke Xia Zhiyang Lu Zhiyang Lu Yong Su Yong Su Weiyun Zhu Weiyun Zhu |
| author_facet | Hongyu Wang Hongyu Wang Pengke Xia Pengke Xia Zhiyang Lu Zhiyang Lu Yong Su Yong Su Weiyun Zhu Weiyun Zhu |
| author_sort | Hongyu Wang |
| collection | DOAJ |
| description | Time-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of microbiome and metabolome induced by TRF based on a growing-pig model. Twelve crossbred growing barrows were randomly allotted into two groups with six replicates (1 pig/pen), namely, the free-access feeding group (FA) and TRF group. Pigs in the FA group were fed free access while the TRF group were fed free access within a regular time three times per day at 07:00–08:00, 12:00–13:00, and 18:00–19:00, respectively. Results showed that the concentrations of NH4-N, putrescine, cadaverine, spermidine, spermine, total biogenic amines, isobutyrate, butyrate, isovalerate, total SCFA, and lactate were increased while the pH value in the colonic digesta and the concentration of acetate was decreased in the TRF group. The Shannon index was significantly increased in the TRF group; however, no significant effects were found in the Fisher index, Simpson index, ACE index, Chao1 index, and observed species between the two groups. In the TRF group, the relative abundances of Prevotella 1 and Eubacterium ruminantium group were significantly increased while the relative abundances of Clostridium sensu sticto 1, Lactobacillus, and Eubacterium coprostanoligenes group were decreased compared with the FA group. PLS-DA analysis revealed an obvious and regular variation between the FA and TRF groups, further pathway enrichment analysis showed that these differential features were mainly enriched in pyrimidine metabolism, nicotinate and nicotinamide metabolism, glycerolipid metabolism, and fructose and mannose metabolism. In addition, Pearson's correlation analysis indicated that the changes in the microbial genera were correlated with the colonic metabolites. In conclusion, these results together indicated that although the overall microbial composition in the colon was not changed, TRF induced the gradient changes of the nutrients and metabolites which were correlated with certain microbial genera including Lactobacillus, Eubacterium_ruminantium group, Eubacterium coprostanoligenes group, Prevotella 1, and Clostridium sensu sticto 1. However, more studies are needed to understand the impacts of TRF on the health and metabolism of growing pigs. |
| first_indexed | 2024-12-23T19:25:52Z |
| format | Article |
| id | doaj.art-3905f14334de4ce0a1e154da0cfbf7fb |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-23T19:25:52Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-3905f14334de4ce0a1e154da0cfbf7fb2022-12-21T17:34:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-06-01810.3389/fvets.2021.681202681202Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted FeedingHongyu Wang0Hongyu Wang1Pengke Xia2Pengke Xia3Zhiyang Lu4Zhiyang Lu5Yong Su6Yong Su7Weiyun Zhu8Weiyun Zhu9Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, ChinaTime-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of microbiome and metabolome induced by TRF based on a growing-pig model. Twelve crossbred growing barrows were randomly allotted into two groups with six replicates (1 pig/pen), namely, the free-access feeding group (FA) and TRF group. Pigs in the FA group were fed free access while the TRF group were fed free access within a regular time three times per day at 07:00–08:00, 12:00–13:00, and 18:00–19:00, respectively. Results showed that the concentrations of NH4-N, putrescine, cadaverine, spermidine, spermine, total biogenic amines, isobutyrate, butyrate, isovalerate, total SCFA, and lactate were increased while the pH value in the colonic digesta and the concentration of acetate was decreased in the TRF group. The Shannon index was significantly increased in the TRF group; however, no significant effects were found in the Fisher index, Simpson index, ACE index, Chao1 index, and observed species between the two groups. In the TRF group, the relative abundances of Prevotella 1 and Eubacterium ruminantium group were significantly increased while the relative abundances of Clostridium sensu sticto 1, Lactobacillus, and Eubacterium coprostanoligenes group were decreased compared with the FA group. PLS-DA analysis revealed an obvious and regular variation between the FA and TRF groups, further pathway enrichment analysis showed that these differential features were mainly enriched in pyrimidine metabolism, nicotinate and nicotinamide metabolism, glycerolipid metabolism, and fructose and mannose metabolism. In addition, Pearson's correlation analysis indicated that the changes in the microbial genera were correlated with the colonic metabolites. In conclusion, these results together indicated that although the overall microbial composition in the colon was not changed, TRF induced the gradient changes of the nutrients and metabolites which were correlated with certain microbial genera including Lactobacillus, Eubacterium_ruminantium group, Eubacterium coprostanoligenes group, Prevotella 1, and Clostridium sensu sticto 1. However, more studies are needed to understand the impacts of TRF on the health and metabolism of growing pigs.https://www.frontiersin.org/articles/10.3389/fvets.2021.681202/fullgrowing pigsmetabolomemicrobiometime-restricted feedingmicrobial metabolites |
| spellingShingle | Hongyu Wang Hongyu Wang Pengke Xia Pengke Xia Zhiyang Lu Zhiyang Lu Yong Su Yong Su Weiyun Zhu Weiyun Zhu Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding Frontiers in Veterinary Science growing pigs metabolome microbiome time-restricted feeding microbial metabolites |
| title | Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding |
| title_full | Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding |
| title_fullStr | Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding |
| title_full_unstemmed | Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding |
| title_short | Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding |
| title_sort | metabolome microbiome responses of growing pigs induced by time restricted feeding |
| topic | growing pigs metabolome microbiome time-restricted feeding microbial metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.681202/full |
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