The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs
KCNQ5 is suggestively associated with myopia, but its specific role in the myopic process has not been studied further. The aim of this study was to investigate the expression of potassium channel gene KCNQ5 and the changes of K+ microenvironment within the retina of form deprivation myopia (FDM) gu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.790580/full |
| _version_ | 1818970333521117184 |
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| author | Qin Yang Qin Yang Qin Yang Qing Qing Tan Qing Qing Tan Chang Jun Lan Chang Jun Lan Bo Zhen Lv Gui Mei Zhou Gui Mei Zhou Wei Qi Zhong Wei Qi Zhong Wei Qi Zhong Zhi Ming Gu Zhi Ming Gu Zhi Ming Gu Yu Mei Mao Xuan Liao Xuan Liao |
| author_facet | Qin Yang Qin Yang Qin Yang Qing Qing Tan Qing Qing Tan Chang Jun Lan Chang Jun Lan Bo Zhen Lv Gui Mei Zhou Gui Mei Zhou Wei Qi Zhong Wei Qi Zhong Wei Qi Zhong Zhi Ming Gu Zhi Ming Gu Zhi Ming Gu Yu Mei Mao Xuan Liao Xuan Liao |
| author_sort | Qin Yang |
| collection | DOAJ |
| description | KCNQ5 is suggestively associated with myopia, but its specific role in the myopic process has not been studied further. The aim of this study was to investigate the expression of potassium channel gene KCNQ5 and the changes of K+ microenvironment within the retina of form deprivation myopia (FDM) guinea pigs. A total of 60 guinea pigs were randomly divided into the normal control (NC) group, the self-control (SC) group, and the form-deprivation (FD) group for different treatments. Molecular assays and immunohistochemistry (IHC) were conducted to measure the expression and distribution of KCNQ5-related gene and protein in the retina. We determined the K+ concentration in the retina. In addition, the possible effects of form deprivation on potassium ionic currents and the pharmacological sensitivity of KCNQ5 activator Retigabine and inhibitor XE991 to the M-current in RPE cells were investigated using the patch-clamp technique. As a result, FD eyes exhibited more myopic refraction and longer AL. The mRNA and protein levels of KCNQ5 significantly decreased in the FD eyes, but the K+ concentration increased. In addition, the M-type K+ current [IK(M)] density decreased in FD RPE cells, and were activated or inhibited in a concentration-dependent manner due to the addition of Retigabine or XE991. Overall, KCNQ5 was significantly downregulated in the retina of FD guinea pigs, which may be associated with the increasing K+ concentration, decreasing IK(M) density, and elongating ocular axis. It suggested that KCNQ5 may play a role in the process of myopia, and the intervention of potassium channels may contribute to the prevention and control of myopia. |
| first_indexed | 2024-12-20T14:34:49Z |
| format | Article |
| id | doaj.art-3909b3eefd4945f889f2f1ba203246fc |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-20T14:34:49Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-3909b3eefd4945f889f2f1ba203246fc2022-12-21T19:37:30ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-12-011210.3389/fphys.2021.790580790580The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea PigsQin Yang0Qin Yang1Qin Yang2Qing Qing Tan3Qing Qing Tan4Chang Jun Lan5Chang Jun Lan6Bo Zhen Lv7Gui Mei Zhou8Gui Mei Zhou9Wei Qi Zhong10Wei Qi Zhong11Wei Qi Zhong12Zhi Ming Gu13Zhi Ming Gu14Zhi Ming Gu15Yu Mei Mao16Xuan Liao17Xuan Liao18Department of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaThe Translational Medicine Research Center and the Hepatobiliary Research Institute (North Sichuan Medical College), Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaThe Translational Medicine Research Center and the Hepatobiliary Research Institute (North Sichuan Medical College), Nanchong, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaThe Translational Medicine Research Center and the Hepatobiliary Research Institute (North Sichuan Medical College), Nanchong, ChinaThe Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Ophthalmology and Optometry, North Sichuan Medical College, Nanchong, ChinaKCNQ5 is suggestively associated with myopia, but its specific role in the myopic process has not been studied further. The aim of this study was to investigate the expression of potassium channel gene KCNQ5 and the changes of K+ microenvironment within the retina of form deprivation myopia (FDM) guinea pigs. A total of 60 guinea pigs were randomly divided into the normal control (NC) group, the self-control (SC) group, and the form-deprivation (FD) group for different treatments. Molecular assays and immunohistochemistry (IHC) were conducted to measure the expression and distribution of KCNQ5-related gene and protein in the retina. We determined the K+ concentration in the retina. In addition, the possible effects of form deprivation on potassium ionic currents and the pharmacological sensitivity of KCNQ5 activator Retigabine and inhibitor XE991 to the M-current in RPE cells were investigated using the patch-clamp technique. As a result, FD eyes exhibited more myopic refraction and longer AL. The mRNA and protein levels of KCNQ5 significantly decreased in the FD eyes, but the K+ concentration increased. In addition, the M-type K+ current [IK(M)] density decreased in FD RPE cells, and were activated or inhibited in a concentration-dependent manner due to the addition of Retigabine or XE991. Overall, KCNQ5 was significantly downregulated in the retina of FD guinea pigs, which may be associated with the increasing K+ concentration, decreasing IK(M) density, and elongating ocular axis. It suggested that KCNQ5 may play a role in the process of myopia, and the intervention of potassium channels may contribute to the prevention and control of myopia.https://www.frontiersin.org/articles/10.3389/fphys.2021.790580/fullmyopiaKCNQ5potassiumM-currentRetigabineXE991 |
| spellingShingle | Qin Yang Qin Yang Qin Yang Qing Qing Tan Qing Qing Tan Chang Jun Lan Chang Jun Lan Bo Zhen Lv Gui Mei Zhou Gui Mei Zhou Wei Qi Zhong Wei Qi Zhong Wei Qi Zhong Zhi Ming Gu Zhi Ming Gu Zhi Ming Gu Yu Mei Mao Xuan Liao Xuan Liao The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs Frontiers in Physiology myopia KCNQ5 potassium M-current Retigabine XE991 |
| title | The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs |
| title_full | The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs |
| title_fullStr | The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs |
| title_full_unstemmed | The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs |
| title_short | The Changes of KCNQ5 Expression and Potassium Microenvironment in the Retina of Myopic Guinea Pigs |
| title_sort | changes of kcnq5 expression and potassium microenvironment in the retina of myopic guinea pigs |
| topic | myopia KCNQ5 potassium M-current Retigabine XE991 |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.790580/full |
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