Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents
The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alte...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/9/3198 |
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| author | Eun-Soo Lee Yujin Ahn Il-Hong Bae Daejin Min Nok Hyun Park Woonggyu Jung Se-Hwa Kim Yong Deog Hong Won Seok Park Chang Seok Lee |
| author_facet | Eun-Soo Lee Yujin Ahn Il-Hong Bae Daejin Min Nok Hyun Park Woonggyu Jung Se-Hwa Kim Yong Deog Hong Won Seok Park Chang Seok Lee |
| author_sort | Eun-Soo Lee |
| collection | DOAJ |
| description | The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier. |
| first_indexed | 2024-03-10T20:07:16Z |
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| id | doaj.art-390db2f394f045d78d14b37127208577 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:07:16Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-390db2f394f045d78d14b371272085772023-11-19T23:11:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01219319810.3390/ijms21093198Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin EquivalentsEun-Soo Lee0Yujin Ahn1Il-Hong Bae2Daejin Min3Nok Hyun Park4Woonggyu Jung5Se-Hwa Kim6Yong Deog Hong7Won Seok Park8Chang Seok Lee9Amorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, KoreaAmorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaAmorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaAmorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, KoreaCenter for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Daejeon 34113, KoreaAmorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaAmorepacific Corporation R&D Center, Yongin 17074, Gyunggi-do, KoreaDepartment of Beauty and Cosmetic Science, Eulji University, Seongnam 13135, Gyunggi-do, KoreaThe outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier.https://www.mdpi.com/1422-0067/21/9/3198seletinoid Gwound healingkeratinocytehuman skin equivalentsoptical coherence tomographysecond harmonic generation |
| spellingShingle | Eun-Soo Lee Yujin Ahn Il-Hong Bae Daejin Min Nok Hyun Park Woonggyu Jung Se-Hwa Kim Yong Deog Hong Won Seok Park Chang Seok Lee Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents International Journal of Molecular Sciences seletinoid G wound healing keratinocyte human skin equivalents optical coherence tomography second harmonic generation |
| title | Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents |
| title_full | Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents |
| title_fullStr | Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents |
| title_full_unstemmed | Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents |
| title_short | Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents |
| title_sort | synthetic retinoid seletinoid g improves skin barrier function through wound healing and collagen realignment in human skin equivalents |
| topic | seletinoid G wound healing keratinocyte human skin equivalents optical coherence tomography second harmonic generation |
| url | https://www.mdpi.com/1422-0067/21/9/3198 |
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