Preservative Effects of Curcumin on Semen of Hu Sheep
Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of the antioxidant system in sperm and causes lipid oxidative damage, destroying its structure and function. Curcumin is a natural pl...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/6/947 |
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| author | Kaiyuan Ji Jinbo Wei Zhiwei Fan Mengkang Zhu Xin Yuan Sihuan Zhang Shuang Li Han Xu Yinghui Ling |
| author_facet | Kaiyuan Ji Jinbo Wei Zhiwei Fan Mengkang Zhu Xin Yuan Sihuan Zhang Shuang Li Han Xu Yinghui Ling |
| author_sort | Kaiyuan Ji |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of the antioxidant system in sperm and causes lipid oxidative damage, destroying its structure and function. Curcumin is a natural plant extract that neutralizes ROS and enhances the function of endogenous antioxidant enzymes. The effect of curcumin on the preservation of sheep semen has not been reported. This study aims to determine the effects of curcumin on refrigerated sperm (4 °C) and analyze the effects of curcumin on sperm metabolism from a Chinese native sheep (Hu sheep). The results showed that adding curcumin significantly improved (<i>p</i> < 0.05) the viability of refrigerated sperm at an optimal concentration of 20 µmol/L, and the plasma membrane and acrosome integrity in semen were significantly improved (<i>p</i> < 0.05). Adding curcumin to refrigerated semen significantly increased (<i>p</i> < 0.05) the levels of antioxidant enzymes (T-AOC, CAT, and SOD) and significantly decreased (<i>p</i> < 0.05) ROS production. A total of 13,796 metabolites in sperm and 20,581 metabolites in negative groups and curcumin-supplemented groups were identified using liquid chromatography–mass spectrometry. The proportion of lipids and lipid-like molecules among all metabolites in the sperm was the highest, regardless of treatment. We identified 50 differentially expressed metabolites (DEMs) in sperm between the negative control and curcumin-treated groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEMs were mainly enriched in the calcium signaling pathway, phospholipase D signaling pathway, sphingolipid metabolism, steroid hormone biosynthesis, 2-oxocarboxylic acid metabolism, and other metabolic pathways. The findings indicate that the addition of an appropriate concentration (20 µm/L) of curcumin to sheep semen can effectively suppress reactive oxygen species (ROS) production and extend the duration of cryopreservation (4 °C) by modulating the expression of sphingosine-1-phosphate, dehydroepiandrosterone sulfate, phytosphingosine, and other metabolites of semen. This discovery offers a novel approach to enhancing the cryogenic preservation of sheep semen. |
| first_indexed | 2024-04-24T18:37:31Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T18:37:31Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-3911cf8ac64a48ef969b9cbcab5489d62024-03-27T13:17:55ZengMDPI AGAnimals2076-26152024-03-0114694710.3390/ani14060947Preservative Effects of Curcumin on Semen of Hu SheepKaiyuan Ji0Jinbo Wei1Zhiwei Fan2Mengkang Zhu3Xin Yuan4Sihuan Zhang5Shuang Li6Han Xu7Yinghui Ling8College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaReactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of the antioxidant system in sperm and causes lipid oxidative damage, destroying its structure and function. Curcumin is a natural plant extract that neutralizes ROS and enhances the function of endogenous antioxidant enzymes. The effect of curcumin on the preservation of sheep semen has not been reported. This study aims to determine the effects of curcumin on refrigerated sperm (4 °C) and analyze the effects of curcumin on sperm metabolism from a Chinese native sheep (Hu sheep). The results showed that adding curcumin significantly improved (<i>p</i> < 0.05) the viability of refrigerated sperm at an optimal concentration of 20 µmol/L, and the plasma membrane and acrosome integrity in semen were significantly improved (<i>p</i> < 0.05). Adding curcumin to refrigerated semen significantly increased (<i>p</i> < 0.05) the levels of antioxidant enzymes (T-AOC, CAT, and SOD) and significantly decreased (<i>p</i> < 0.05) ROS production. A total of 13,796 metabolites in sperm and 20,581 metabolites in negative groups and curcumin-supplemented groups were identified using liquid chromatography–mass spectrometry. The proportion of lipids and lipid-like molecules among all metabolites in the sperm was the highest, regardless of treatment. We identified 50 differentially expressed metabolites (DEMs) in sperm between the negative control and curcumin-treated groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEMs were mainly enriched in the calcium signaling pathway, phospholipase D signaling pathway, sphingolipid metabolism, steroid hormone biosynthesis, 2-oxocarboxylic acid metabolism, and other metabolic pathways. The findings indicate that the addition of an appropriate concentration (20 µm/L) of curcumin to sheep semen can effectively suppress reactive oxygen species (ROS) production and extend the duration of cryopreservation (4 °C) by modulating the expression of sphingosine-1-phosphate, dehydroepiandrosterone sulfate, phytosphingosine, and other metabolites of semen. This discovery offers a novel approach to enhancing the cryogenic preservation of sheep semen.https://www.mdpi.com/2076-2615/14/6/947sheepspermrosmetabolome |
| spellingShingle | Kaiyuan Ji Jinbo Wei Zhiwei Fan Mengkang Zhu Xin Yuan Sihuan Zhang Shuang Li Han Xu Yinghui Ling Preservative Effects of Curcumin on Semen of Hu Sheep Animals sheep sperm ros metabolome |
| title | Preservative Effects of Curcumin on Semen of Hu Sheep |
| title_full | Preservative Effects of Curcumin on Semen of Hu Sheep |
| title_fullStr | Preservative Effects of Curcumin on Semen of Hu Sheep |
| title_full_unstemmed | Preservative Effects of Curcumin on Semen of Hu Sheep |
| title_short | Preservative Effects of Curcumin on Semen of Hu Sheep |
| title_sort | preservative effects of curcumin on semen of hu sheep |
| topic | sheep sperm ros metabolome |
| url | https://www.mdpi.com/2076-2615/14/6/947 |
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