Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae
ABSTRACT: Objectives: This study reports identification of the carbapenemase-encoding gene from carbapenem-resistant Enterobacterales from food animals. Methods: A total of 40 bacterial isolates recovered from 475 faecal swabs obtained on one farm were tested for the presence of the blaNDM-1 gene b...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521001594 |
| _version_ | 1819112723334561792 |
|---|---|
| author | Xing-Run Zheng Yin-Huan Sun Jia-Hang Zhu Si-Li Wu Cai Ping Liang-Xing Fang Hong-Xia Jiang |
| author_facet | Xing-Run Zheng Yin-Huan Sun Jia-Hang Zhu Si-Li Wu Cai Ping Liang-Xing Fang Hong-Xia Jiang |
| author_sort | Xing-Run Zheng |
| collection | DOAJ |
| description | ABSTRACT: Objectives: This study reports identification of the carbapenemase-encoding gene from carbapenem-resistant Enterobacterales from food animals. Methods: A total of 40 bacterial isolates recovered from 475 faecal swabs obtained on one farm were tested for the presence of the blaNDM-1 gene by PCR. Species identification of three blaNDM-1-positive strains was conducted by MALDI-TOF/MS. Antimicrobial susceptibility testing was performed by broth microdilution. Transferability of the blaNDM-1 and cfr genes was determined by filter mating. The genetic environment of blaNDM-1 and cfr was analysed by whole-genome sequencing. Results: Two Proteus mirabilis (JPM24 and YPM35) and one Providencia rettgeri (YPR25) carried blaNDM-1. The blaNDM-1 genes were located on conjugable pPrY2001-like plasmids often reported to carry important antimicrobial resistance genes (ARGs). YPR25 and YPM35 shared two almost identical conjugable plasmids, one carrying blaNDM-1 and the other cfr. The blaNDM-1 gene in YPR25 (same as YPM35) and JPM24 was located in two novel transposons, designated Tn6922 and Tn6923, respectively. Tn6922 and Tn6923 carried 14 and 7 ARGs, respectively, and both contained multiple copies of IS26 in the same direction, with a high degree of similarity. Additionally, cfr was located on a plasmid with an unreported high frequency of conjugative transfer in YPR25 (same as YPM35). Conclusion: We identified two novel blaNDM-1-containing transposons (Tn6922 and Tn6923) present on pPrY2001-like plasmids. The pPrY2001-like blaNDM-1 plasmids coexisted with a novel cfr plasmid, and both could transfer at high frequency, highlighting the importance of continuous surveillance of multiresistant Enterobacterales of animal origin that can serve as a reservoir for ARGs. |
| first_indexed | 2024-12-22T04:18:02Z |
| format | Article |
| id | doaj.art-39174442f0534766a296820f0b9b12f0 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-22T04:18:02Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-39174442f0534766a296820f0b9b12f02022-12-21T18:39:21ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-09-0126222226Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source EnterobacteriaceaeXing-Run Zheng0Yin-Huan Sun1Jia-Hang Zhu2Si-Li Wu3Cai Ping4Liang-Xing Fang5Hong-Xia Jiang6Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Corresponding author. Mailing address: National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Origin Bacteria, College of Veterinary Medicine, South China Agricultural University (SCAU), 483 Wushan Road, Guangzhou 510642, China. Tel.: +86 20 8528 3934.ABSTRACT: Objectives: This study reports identification of the carbapenemase-encoding gene from carbapenem-resistant Enterobacterales from food animals. Methods: A total of 40 bacterial isolates recovered from 475 faecal swabs obtained on one farm were tested for the presence of the blaNDM-1 gene by PCR. Species identification of three blaNDM-1-positive strains was conducted by MALDI-TOF/MS. Antimicrobial susceptibility testing was performed by broth microdilution. Transferability of the blaNDM-1 and cfr genes was determined by filter mating. The genetic environment of blaNDM-1 and cfr was analysed by whole-genome sequencing. Results: Two Proteus mirabilis (JPM24 and YPM35) and one Providencia rettgeri (YPR25) carried blaNDM-1. The blaNDM-1 genes were located on conjugable pPrY2001-like plasmids often reported to carry important antimicrobial resistance genes (ARGs). YPR25 and YPM35 shared two almost identical conjugable plasmids, one carrying blaNDM-1 and the other cfr. The blaNDM-1 gene in YPR25 (same as YPM35) and JPM24 was located in two novel transposons, designated Tn6922 and Tn6923, respectively. Tn6922 and Tn6923 carried 14 and 7 ARGs, respectively, and both contained multiple copies of IS26 in the same direction, with a high degree of similarity. Additionally, cfr was located on a plasmid with an unreported high frequency of conjugative transfer in YPR25 (same as YPM35). Conclusion: We identified two novel blaNDM-1-containing transposons (Tn6922 and Tn6923) present on pPrY2001-like plasmids. The pPrY2001-like blaNDM-1 plasmids coexisted with a novel cfr plasmid, and both could transfer at high frequency, highlighting the importance of continuous surveillance of multiresistant Enterobacterales of animal origin that can serve as a reservoir for ARGs.http://www.sciencedirect.com/science/article/pii/S2213716521001594TransposonPlasmidCarbapenem |
| spellingShingle | Xing-Run Zheng Yin-Huan Sun Jia-Hang Zhu Si-Li Wu Cai Ping Liang-Xing Fang Hong-Xia Jiang Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae Journal of Global Antimicrobial Resistance Transposon Plasmid Carbapenem |
| title | Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae |
| title_full | Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae |
| title_fullStr | Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae |
| title_full_unstemmed | Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae |
| title_short | Two novel blaNDM-1-harbouring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food animal source Enterobacteriaceae |
| title_sort | two novel blandm 1 harbouring transposons on ppry2001 like plasmids coexisting with a novel cfr encoding plasmid in food animal source enterobacteriaceae |
| topic | Transposon Plasmid Carbapenem |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521001594 |
| work_keys_str_mv | AT xingrunzheng twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT yinhuansun twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT jiahangzhu twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT siliwu twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT caiping twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT liangxingfang twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae AT hongxiajiang twonovelblandm1harbouringtransposonsonppry2001likeplasmidscoexistingwithanovelcfrencodingplasmidinfoodanimalsourceenterobacteriaceae |