Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom
We showed recently that nerve growth factor (NGF) from cobra venom inhibited the growth of Ehrlich ascites carcinoma (EAC) inoculated subcutaneously in mice. Here, we studied the influence of anti-complementary cobra venom factor (CVF) and the non-steroidal anti-inflammatory drug ketoprofen on the a...
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MDPI AG
2017-09-01
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| Series: | Toxins |
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| author | Alexey V. Osipov Tatiana I. Terpinskaya Tatiana E. Kuznetsova Elena L. Ryzhkovskaya Vladimir S. Lukashevich Julia A. Rudnichenko Vladimir S. Ulashchyk Vladislav G. Starkov Yuri N. Utkin |
| author_facet | Alexey V. Osipov Tatiana I. Terpinskaya Tatiana E. Kuznetsova Elena L. Ryzhkovskaya Vladimir S. Lukashevich Julia A. Rudnichenko Vladimir S. Ulashchyk Vladislav G. Starkov Yuri N. Utkin |
| author_sort | Alexey V. Osipov |
| collection | DOAJ |
| description | We showed recently that nerve growth factor (NGF) from cobra venom inhibited the growth of Ehrlich ascites carcinoma (EAC) inoculated subcutaneously in mice. Here, we studied the influence of anti-complementary cobra venom factor (CVF) and the non-steroidal anti-inflammatory drug ketoprofen on the antitumor NGF effect, as well as on NGF-induced changes in EAC histological patterns, the activity of lactate and succinate dehydrogenases in tumor cells and the serum level of some cytokines. NGF, CVF and ketoprofen reduced the tumor volume by approximately 72%, 68% and 30%, respectively. The antitumor effect of NGF was accompanied by an increase in the lymphocytic infiltration of the tumor tissue, the level of interleukin 1β and tumor necrosis factor α in the serum, as well as the activity of lactate and succinate dehydrogenases in tumor cells. Simultaneous administration of NGF with either CVF or ketoprofen abolished the antitumor effect and reduced all other effects of NGF, whereas NGF itself significantly decreased the antitumor action of both CVF and ketoprofen. Thus, the antitumor effect of NGF critically depended on the status of the immune system and was abolished by the disturbance of the complement system; the disturbance of the inflammatory response canceled the antitumor effect as well. |
| first_indexed | 2024-04-11T21:48:22Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-04-11T21:48:22Z |
| publishDate | 2017-09-01 |
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| series | Toxins |
| spelling | doaj.art-391b391273f34377b83800655e3c3ab02022-12-22T04:01:19ZengMDPI AGToxins2072-66512017-09-019927410.3390/toxins9090274toxins9090274Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra VenomAlexey V. Osipov0Tatiana I. Terpinskaya1Tatiana E. Kuznetsova2Elena L. Ryzhkovskaya3Vladimir S. Lukashevich4Julia A. Rudnichenko5Vladimir S. Ulashchyk6Vladislav G. Starkov7Yuri N. Utkin8Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusInstitute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, BelarusShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, RussiaWe showed recently that nerve growth factor (NGF) from cobra venom inhibited the growth of Ehrlich ascites carcinoma (EAC) inoculated subcutaneously in mice. Here, we studied the influence of anti-complementary cobra venom factor (CVF) and the non-steroidal anti-inflammatory drug ketoprofen on the antitumor NGF effect, as well as on NGF-induced changes in EAC histological patterns, the activity of lactate and succinate dehydrogenases in tumor cells and the serum level of some cytokines. NGF, CVF and ketoprofen reduced the tumor volume by approximately 72%, 68% and 30%, respectively. The antitumor effect of NGF was accompanied by an increase in the lymphocytic infiltration of the tumor tissue, the level of interleukin 1β and tumor necrosis factor α in the serum, as well as the activity of lactate and succinate dehydrogenases in tumor cells. Simultaneous administration of NGF with either CVF or ketoprofen abolished the antitumor effect and reduced all other effects of NGF, whereas NGF itself significantly decreased the antitumor action of both CVF and ketoprofen. Thus, the antitumor effect of NGF critically depended on the status of the immune system and was abolished by the disturbance of the complement system; the disturbance of the inflammatory response canceled the antitumor effect as well.https://www.mdpi.com/2072-6651/9/9/274Ehrlich carcinomaimmune systemnerve growth factorcobra venomcobra venom factorketoprofen |
| spellingShingle | Alexey V. Osipov Tatiana I. Terpinskaya Tatiana E. Kuznetsova Elena L. Ryzhkovskaya Vladimir S. Lukashevich Julia A. Rudnichenko Vladimir S. Ulashchyk Vladislav G. Starkov Yuri N. Utkin Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom Toxins Ehrlich carcinoma immune system nerve growth factor cobra venom cobra venom factor ketoprofen |
| title | Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom |
| title_full | Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom |
| title_fullStr | Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom |
| title_full_unstemmed | Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom |
| title_short | Cobra Venom Factor and Ketoprofen Abolish the Antitumor Effect of Nerve Growth Factor from Cobra Venom |
| title_sort | cobra venom factor and ketoprofen abolish the antitumor effect of nerve growth factor from cobra venom |
| topic | Ehrlich carcinoma immune system nerve growth factor cobra venom cobra venom factor ketoprofen |
| url | https://www.mdpi.com/2072-6651/9/9/274 |
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