Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i>
Phycoerythrin, a special photosynthetic pigment, is widely used as fluorescent dye and has lots of underlying beneficial effects on health. A marine red microalga <i>Porphyridium</i> is considered as the potential feedstock for phycoerythrin production. However, the phycoerythrin-related...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/14/2069 |
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| author | Chulin Li Houbo Wu Wenzhou Xiang Hualian Wu Na Wang Jiayi Wu Tao Li |
| author_facet | Chulin Li Houbo Wu Wenzhou Xiang Hualian Wu Na Wang Jiayi Wu Tao Li |
| author_sort | Chulin Li |
| collection | DOAJ |
| description | Phycoerythrin, a special photosynthetic pigment, is widely used as fluorescent dye and has lots of underlying beneficial effects on health. A marine red microalga <i>Porphyridium</i> is considered as the potential feedstock for phycoerythrin production. However, the phycoerythrin-related properties of <i>Porphyridium</i> have not been systematically evaluated, especially between the species of <i>P. cruentum</i> and <i>P. purpureum</i>. The present study aimed to evaluate the production and fluorescence characteristics of phycoerythrin of three strains of <i>Porphyridium</i>. The results showed that <i>P. purpureum</i> SCS-02 presented the highest biomass, phycoerythrin content and yield were 6.43 g L<sup>−1</sup>, 9.18% DW and 0.288 g L<sup>−1</sup>, respectively. There was no significant difference between <i>P. purpureum</i> and <i>P. cruentum</i> in α and β subunits amino acid sequences of phycoerythrin and in fluorescence characteristics. The high gene expression level of the key enzymes in phycoerythrobilin synthesis (porphobilinogen synthase and oxygen-dependent coproporphyrinogen-III oxidase) could be related to the high phycoerythrin content of <i>Porphyridium</i>. Based on systematic evaluation, <i>P. purpureum</i> SCS-02 was selected due to its high biomass and phycoerythrin yield. <i>P. purpureum</i> and <i>P. cruentum</i> were highly similar in the phylogenetic tree, as well as in fluorescence characteristics; therefore, it was speculated that they might be the same <i>Porphyridium</i> species. |
| first_indexed | 2024-03-09T10:19:05Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T10:19:05Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-391d64a4f41a4693aece0f51688a6fb72023-12-01T22:09:01ZengMDPI AGFoods2304-81582022-07-011114206910.3390/foods11142069Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i>Chulin Li0Houbo Wu1Wenzhou Xiang2Hualian Wu3Na Wang4Jiayi Wu5Tao Li6CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaPhycoerythrin, a special photosynthetic pigment, is widely used as fluorescent dye and has lots of underlying beneficial effects on health. A marine red microalga <i>Porphyridium</i> is considered as the potential feedstock for phycoerythrin production. However, the phycoerythrin-related properties of <i>Porphyridium</i> have not been systematically evaluated, especially between the species of <i>P. cruentum</i> and <i>P. purpureum</i>. The present study aimed to evaluate the production and fluorescence characteristics of phycoerythrin of three strains of <i>Porphyridium</i>. The results showed that <i>P. purpureum</i> SCS-02 presented the highest biomass, phycoerythrin content and yield were 6.43 g L<sup>−1</sup>, 9.18% DW and 0.288 g L<sup>−1</sup>, respectively. There was no significant difference between <i>P. purpureum</i> and <i>P. cruentum</i> in α and β subunits amino acid sequences of phycoerythrin and in fluorescence characteristics. The high gene expression level of the key enzymes in phycoerythrobilin synthesis (porphobilinogen synthase and oxygen-dependent coproporphyrinogen-III oxidase) could be related to the high phycoerythrin content of <i>Porphyridium</i>. Based on systematic evaluation, <i>P. purpureum</i> SCS-02 was selected due to its high biomass and phycoerythrin yield. <i>P. purpureum</i> and <i>P. cruentum</i> were highly similar in the phylogenetic tree, as well as in fluorescence characteristics; therefore, it was speculated that they might be the same <i>Porphyridium</i> species.https://www.mdpi.com/2304-8158/11/14/2069<i>Porphyridium</i>phycoerythrinfluorescence characteristicsRNA-seq |
| spellingShingle | Chulin Li Houbo Wu Wenzhou Xiang Hualian Wu Na Wang Jiayi Wu Tao Li Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> Foods <i>Porphyridium</i> phycoerythrin fluorescence characteristics RNA-seq |
| title | Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> |
| title_full | Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> |
| title_fullStr | Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> |
| title_full_unstemmed | Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> |
| title_short | Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of <i>Porphyridium</i> |
| title_sort | comparison of production and fluorescence characteristics of phycoerythrin from three strains of i porphyridium i |
| topic | <i>Porphyridium</i> phycoerythrin fluorescence characteristics RNA-seq |
| url | https://www.mdpi.com/2304-8158/11/14/2069 |
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