High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China
Abstract Objectives Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Provi...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-024-03232-5 |
| _version_ | 1797259486542430208 |
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| author | Qing Zhan Gaoqin Teng Weiwei Chen Xiao Yu |
| author_facet | Qing Zhan Gaoqin Teng Weiwei Chen Xiao Yu |
| author_sort | Qing Zhan |
| collection | DOAJ |
| description | Abstract Objectives Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China. Methods From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6 hospitals in 4 cities in Zhejiang province, east China. Antimicrobial susceptibility tests were conducted according to the guidelines set forth by the Clinical and Laboratory Standards Institute (CLSI). To characterize and analyze these isolates, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing and virulence genes gene profiles were performed. Results The most predominant clone was ST5-SCCmec II-t311, which accounted for 41.8% (110/263), followed by ST59 (44/263, 16.7%). Compared with non-ST5-II-t311 isolates, ST5-II-t311 isolates were more resistant to erythromycin, tetracycline, levofloxacin, moxifloxacin, and ciprofloxacin, but more susceptible to clindamycin. Moreover, the rates of multidrug resistance were higher in ST5-II-t311 isolates compared to the non-ST5-II-t311 isolates. In comparison to the non-ST5-II-t311 isolates, ST5-II-t311 isolates showed no significant difference in virulence genes detected. Conclusions MRSA ST5-II-t311 clone has become the most predominant clone in Zhejiang Province, east China and has higher rates of multidrug resistance than other isolates, that should be kept in mind when treating BSI. Moreover, MRSA ST59 clone shows an upward trend and has begun to spread into hospitals. Our findings highlight the importance of epidemiological studies of S. aureus carriage in the eastern region. |
| first_indexed | 2024-04-24T23:10:12Z |
| format | Article |
| id | doaj.art-391efeeb50424758ba0a125495ec7fe0 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-24T23:10:12Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-391efeeb50424758ba0a125495ec7fe02024-03-17T12:17:17ZengBMCBMC Microbiology1471-21802024-03-0124111010.1186/s12866-024-03232-5High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East ChinaQing Zhan0Gaoqin Teng1Weiwei Chen2Xiao Yu3Infection Control Department, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of General Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Clinical Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of MedicineNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, The First Hospital of Shanxi Medical UniversityAbstract Objectives Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China. Methods From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6 hospitals in 4 cities in Zhejiang province, east China. Antimicrobial susceptibility tests were conducted according to the guidelines set forth by the Clinical and Laboratory Standards Institute (CLSI). To characterize and analyze these isolates, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing and virulence genes gene profiles were performed. Results The most predominant clone was ST5-SCCmec II-t311, which accounted for 41.8% (110/263), followed by ST59 (44/263, 16.7%). Compared with non-ST5-II-t311 isolates, ST5-II-t311 isolates were more resistant to erythromycin, tetracycline, levofloxacin, moxifloxacin, and ciprofloxacin, but more susceptible to clindamycin. Moreover, the rates of multidrug resistance were higher in ST5-II-t311 isolates compared to the non-ST5-II-t311 isolates. In comparison to the non-ST5-II-t311 isolates, ST5-II-t311 isolates showed no significant difference in virulence genes detected. Conclusions MRSA ST5-II-t311 clone has become the most predominant clone in Zhejiang Province, east China and has higher rates of multidrug resistance than other isolates, that should be kept in mind when treating BSI. Moreover, MRSA ST59 clone shows an upward trend and has begun to spread into hospitals. Our findings highlight the importance of epidemiological studies of S. aureus carriage in the eastern region.https://doi.org/10.1186/s12866-024-03232-5Methicillin-resistant staphylococcus aureus (MRSA)ST5-SCCmec II-t311Bloodstream infections (BSIs)Antimicrobial susceptibilityVirulence genes |
| spellingShingle | Qing Zhan Gaoqin Teng Weiwei Chen Xiao Yu High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China BMC Microbiology Methicillin-resistant staphylococcus aureus (MRSA) ST5-SCCmec II-t311 Bloodstream infections (BSIs) Antimicrobial susceptibility Virulence genes |
| title | High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China |
| title_full | High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China |
| title_fullStr | High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China |
| title_full_unstemmed | High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China |
| title_short | High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China |
| title_sort | high prevalence of st5 sccmec ii t311 clone of methicillin resistant staphylococcus aureus isolated from bloodstream infections in east china |
| topic | Methicillin-resistant staphylococcus aureus (MRSA) ST5-SCCmec II-t311 Bloodstream infections (BSIs) Antimicrobial susceptibility Virulence genes |
| url | https://doi.org/10.1186/s12866-024-03232-5 |
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