Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method
Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mea...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1997-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100020 |
| _version_ | 1797757245968089088 |
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| author | Mehnert DU Stewien KE Hársi CM Queiroz APS Candeias JMG Candeias JAN |
| author_facet | Mehnert DU Stewien KE Hársi CM Queiroz APS Candeias JMG Candeias JAN |
| author_sort | Mehnert DU |
| collection | DOAJ |
| description | Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% ± 7.5 of the SA-11 virus, was achieved |
| first_indexed | 2024-03-12T18:13:06Z |
| format | Article |
| id | doaj.art-3920154829024955b9522e33d941f791 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:13:06Z |
| publishDate | 1997-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3920154829024955b9522e33d941f7912023-08-02T09:14:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-01-0192197100Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration MethodMehnert DUStewien KEHársi CMQueiroz APSCandeias JMGCandeias JANSimian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% ± 7.5 of the SA-11 virus, was achievedhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100020rotavirussewagevirus concentrationdirect immunoperoxidase |
| spellingShingle | Mehnert DU Stewien KE Hársi CM Queiroz APS Candeias JMG Candeias JAN Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method Memorias do Instituto Oswaldo Cruz rotavirus sewage virus concentration direct immunoperoxidase |
| title | Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method |
| title_full | Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method |
| title_fullStr | Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method |
| title_full_unstemmed | Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method |
| title_short | Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method |
| title_sort | detection of rotavirus in sewage and creek water efficiency of the concentration method |
| topic | rotavirus sewage virus concentration direct immunoperoxidase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100020 |
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