HBD-2 interactions with erythrocyte membranes in vitro
HBD-2 – a member of β-defensin family of antimicrobial peptides – is known to permeabilize cell membranes of susceptible cells, but the mechanism of such interactions is poorly understood. In our study, we have used a hemolytic model to explore the kinetic properties of HBD-2 interactions with membr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2019-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2019/01/Makarenko_1_19.pdf |
| _version_ | 1797668827250556928 |
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| author | M. V. Makarenko D. O. Semeniuk I. O. Starenka A. P. Pogribna I. V. Sokol L. I. Martinova D. O. Govsieiev |
| author_facet | M. V. Makarenko D. O. Semeniuk I. O. Starenka A. P. Pogribna I. V. Sokol L. I. Martinova D. O. Govsieiev |
| author_sort | M. V. Makarenko |
| collection | DOAJ |
| description | HBD-2 – a member of β-defensin family of antimicrobial peptides – is known to permeabilize cell membranes of susceptible cells, but the mechanism of such interactions is poorly understood. In our study, we have used a hemolytic model to explore the kinetic properties of HBD-2 interactions with membranes of human erythrocytes. We ran hemolytic assays with a wide range of both HBD-2 and erythrocyte concentrations, as well as varying pH values, incubation times, and osmotic strengths; each in the presence or the absence of inhibitory substances such as proteins and salts. The results show that HBD-2 cell membrane permeabilization is both dose- and time-dependent (with plateau effect observed in each case), and inversely dependent on erythrocyte concentration. HBD-2 interactions with cell membranes highly depend on pH value and the presence of inhibitors but are not affected by tested osmotic strength range. Our findings suggest that interactions of HBD-2 with cell membranes are mainly electrostatic in nature and are limited by released cell content. We have developed a speculative model of such interaction based on our results. |
| first_indexed | 2024-03-11T20:34:08Z |
| format | Article |
| id | doaj.art-3921c804f6744d82bfbc55ae3329f94a |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T20:34:08Z |
| publishDate | 2019-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-3921c804f6744d82bfbc55ae3329f94a2023-10-02T07:19:18ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032019-02-01911657310.15407/ubj91.01.065HBD-2 interactions with erythrocyte membranes in vitroM. V. Makarenko0D. O. Semeniuk1I. O. Starenka 2A. P. Pogribna3I. V. Sokol4 L. I. Martinova5D. O. Govsieiev6Institute of Postgraduate Education, Bogomolets National Medical University, Kyiv UkraineESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, UkraineESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, UkraineInstitute of Molecular Biology and Genetics, NAS of Ukraine, KyivKyiv City Maternity Hospital No 5Institute of Postgraduate Education, Bogomolets National Medical University, Kyiv UkraineKyiv City Maternity Hospital No 5HBD-2 – a member of β-defensin family of antimicrobial peptides – is known to permeabilize cell membranes of susceptible cells, but the mechanism of such interactions is poorly understood. In our study, we have used a hemolytic model to explore the kinetic properties of HBD-2 interactions with membranes of human erythrocytes. We ran hemolytic assays with a wide range of both HBD-2 and erythrocyte concentrations, as well as varying pH values, incubation times, and osmotic strengths; each in the presence or the absence of inhibitory substances such as proteins and salts. The results show that HBD-2 cell membrane permeabilization is both dose- and time-dependent (with plateau effect observed in each case), and inversely dependent on erythrocyte concentration. HBD-2 interactions with cell membranes highly depend on pH value and the presence of inhibitors but are not affected by tested osmotic strength range. Our findings suggest that interactions of HBD-2 with cell membranes are mainly electrostatic in nature and are limited by released cell content. We have developed a speculative model of such interaction based on our results.http://ukrbiochemjournal.org/wp-content/uploads/2019/01/Makarenko_1_19.pdfhemolytic activityhuman beta-defensin 2membrane permeabilization |
| spellingShingle | M. V. Makarenko D. O. Semeniuk I. O. Starenka A. P. Pogribna I. V. Sokol L. I. Martinova D. O. Govsieiev HBD-2 interactions with erythrocyte membranes in vitro The Ukrainian Biochemical Journal hemolytic activity human beta-defensin 2 membrane permeabilization |
| title | HBD-2 interactions with erythrocyte membranes in vitro |
| title_full | HBD-2 interactions with erythrocyte membranes in vitro |
| title_fullStr | HBD-2 interactions with erythrocyte membranes in vitro |
| title_full_unstemmed | HBD-2 interactions with erythrocyte membranes in vitro |
| title_short | HBD-2 interactions with erythrocyte membranes in vitro |
| title_sort | hbd 2 interactions with erythrocyte membranes in vitro |
| topic | hemolytic activity human beta-defensin 2 membrane permeabilization |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2019/01/Makarenko_1_19.pdf |
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