Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population
Background: The inability of young children to expectorate sputum and paucibacillary status of Mycobacterium tuberculosis (MTB) increases its diagnostic complexity. In this study, we aimed to standardize a stool concentration method for the detection of MTB and its drug resistance by line probe assa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=4;spage=371;epage=377;aulast=Rajendran |
| _version_ | 1811296066007465984 |
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| author | Priya Rajendran Baskaran Murugesan Sarath Balaji Sivakumar Shanmugam Sivaraman Palanisamy Thirumalani Ramamoorthy Sindhu Hasini Bella Devaleenal Basilea Watson |
| author_facet | Priya Rajendran Baskaran Murugesan Sarath Balaji Sivakumar Shanmugam Sivaraman Palanisamy Thirumalani Ramamoorthy Sindhu Hasini Bella Devaleenal Basilea Watson |
| author_sort | Priya Rajendran |
| collection | DOAJ |
| description | Background: The inability of young children to expectorate sputum and paucibacillary status of Mycobacterium tuberculosis (MTB) increases its diagnostic complexity. In this study, we aimed to standardize a stool concentration method for the detection of MTB and its drug resistance by line probe assay (LPA). Methods: The stool from 10 healthy children spiked with H37Rv in five different dilutions (1:1, 1:10, 1:100, 1:1000, and 1:10,000), and stool from 10 confirmed TB and 54 clinically diagnosed TB children were subjected to an in-house stool concentration protocol. All the processed filtrates were subjected to smear microscopy, solid culture, Xpert ultra testing, and LPA. Results: Of 10 control samples, growth was seen in four samples (neat 1:1). In smear microscopy, bacilli could be seen in eight samples (1:1 and 1:10). Xpert ultra testing could detect MTB in eight samples in all dilutions with different loads. LPA could detect MTB in all samples and dilutions. In microbiologically confirmed children, seven out of 10 stool samples tested were positive. Out of 54 children with clinically diagnosed TB, 4 (7.4%) could be confirmed by microbiological diagnosis. Conclusion: The protocol standardized in this study proves to be better working in the molecular detection of MTB. |
| first_indexed | 2024-04-13T05:43:52Z |
| format | Article |
| id | doaj.art-39261347ea0d4792a1a4555332ccc845 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-04-13T05:43:52Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-39261347ea0d4792a1a4555332ccc8452022-12-22T03:00:01ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2022-01-0111437137710.4103/ijmy.ijmy_126_22Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric populationPriya RajendranBaskaran MurugesanSarath BalajiSivakumar ShanmugamSivaraman PalanisamyThirumalani RamamoorthySindhu HasiniBella DevaleenalBasilea WatsonBackground: The inability of young children to expectorate sputum and paucibacillary status of Mycobacterium tuberculosis (MTB) increases its diagnostic complexity. In this study, we aimed to standardize a stool concentration method for the detection of MTB and its drug resistance by line probe assay (LPA). Methods: The stool from 10 healthy children spiked with H37Rv in five different dilutions (1:1, 1:10, 1:100, 1:1000, and 1:10,000), and stool from 10 confirmed TB and 54 clinically diagnosed TB children were subjected to an in-house stool concentration protocol. All the processed filtrates were subjected to smear microscopy, solid culture, Xpert ultra testing, and LPA. Results: Of 10 control samples, growth was seen in four samples (neat 1:1). In smear microscopy, bacilli could be seen in eight samples (1:1 and 1:10). Xpert ultra testing could detect MTB in eight samples in all dilutions with different loads. LPA could detect MTB in all samples and dilutions. In microbiologically confirmed children, seven out of 10 stool samples tested were positive. Out of 54 children with clinically diagnosed TB, 4 (7.4%) could be confirmed by microbiological diagnosis. Conclusion: The protocol standardized in this study proves to be better working in the molecular detection of MTB.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=4;spage=371;epage=377;aulast=Rajendranconcentrationline probe assaypediatric tuberculosisstool |
| spellingShingle | Priya Rajendran Baskaran Murugesan Sarath Balaji Sivakumar Shanmugam Sivaraman Palanisamy Thirumalani Ramamoorthy Sindhu Hasini Bella Devaleenal Basilea Watson Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population International Journal of Mycobacteriology concentration line probe assay pediatric tuberculosis stool |
| title | Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population |
| title_full | Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population |
| title_fullStr | Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population |
| title_full_unstemmed | Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population |
| title_short | Standardization of a stool concentration method for Mycobacterium tuberculosis detection in the pediatric population |
| title_sort | standardization of a stool concentration method for mycobacterium tuberculosis detection in the pediatric population |
| topic | concentration line probe assay pediatric tuberculosis stool |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=4;spage=371;epage=377;aulast=Rajendran |
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