miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway
Abstract Background We have previously verified the beneficial effects of exosomes from endothelial progenitor cells (EPC-EXs) in ischemic stroke. However, the effects of EPC-EXs in hemorrhagic stroke have not been investigated. Additionally, miR-137 is reported to regulate ferroptosis and to be inv...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-020-01836-y |
| _version_ | 1818192477314613248 |
|---|---|
| author | Yuchen Li Jinju Wang Shuzhen Chen Pei Wu Shancai Xu Chunlei Wang Huaizhang Shi Ji Bihl |
| author_facet | Yuchen Li Jinju Wang Shuzhen Chen Pei Wu Shancai Xu Chunlei Wang Huaizhang Shi Ji Bihl |
| author_sort | Yuchen Li |
| collection | DOAJ |
| description | Abstract Background We have previously verified the beneficial effects of exosomes from endothelial progenitor cells (EPC-EXs) in ischemic stroke. However, the effects of EPC-EXs in hemorrhagic stroke have not been investigated. Additionally, miR-137 is reported to regulate ferroptosis and to be involved in the neuroprotection against ischemic stroke. Hence, the present work explored the effects of miR-137-overexpressing EPC-EXs on apoptosis, mitochondrial dysfunction, and ferroptosis in oxyhemoglobin (oxyHb)-injured SH-SY5Y cells. Methods The lentiviral miR-137 was transfected into EPCs and then the EPC-EXs were collected. RT-PCR was used to detect the miR-137 level in EPCs, EXs, and neurons. The uptake mechanisms of EPC-EXs in SH-SY5Y cells were explored by the co-incubation of Dynasore, Pitstop 2, Ly294002, and Genistein. After the transfection of different types of EPC-EXs, flow cytometry and expression of cytochrome c and cleaved caspase-3 were used to detect the apoptosis of oxyHb-injured neurons. Neuronal mitochondrial function was assessed by reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP) depolarization, and cellular ATP content. Cell ferroptosis was measured by lipid peroxidation, iron overload, degradation of glutathione, and glutathione peroxidase 4. Additionally, recombinational PGE2 was used to detect if activation of COX2/PGE2 pathway could reverse the protection of miR-137 overexpression. Results The present work showed (1) EPC-EXs could be taken in by SH-SY5Y cells via caveolin-/clathrin-mediated pathways and macropinocytosis; (2) miR-137 was decreased in neurons after oxyHb treatment, and EXsmiR-137 could restore the miR-137 levels; (3) EXsmiR-137 worked better than EXs in reducing the number of apoptotic neurons and pro-apoptotic protein expression after oxyHb treatment; (4) EXsmiR-137 are more effective in improving the cellular MMP, ROS, and ATP level; (5) EXsmiR-137, but not EXs, protected oxyHb-treated SH-SY5Y cells against lipid peroxidation, iron overload, degradation of glutathione, and glutathione peroxidase 4; and (6) EXsmiR-137 suppressed the expression of the COX2/PGE2 pathway, and activation of the pathway could partially reverse the neuroprotective effects of EXsmiR-137. Conclusion miR-137 overexpression boosts the neuroprotective effects of EPC-EXs against apoptosis and mitochondrial dysfunction in oxyHb-treated SH-SY5Y cells. Furthermore, EXsmiR-137 rather than EXs can restore the decrease in miR-137 levels and inhibit ferroptosis, and the protection mechanism might involve the miR-137-COX2/PGE2 signaling pathway. |
| first_indexed | 2024-12-12T00:31:07Z |
| format | Article |
| id | doaj.art-3927413e25c34e8ba4b1c5ae94bec744 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-12T00:31:07Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-3927413e25c34e8ba4b1c5ae94bec7442022-12-22T00:44:29ZengBMCStem Cell Research & Therapy1757-65122020-10-0111111810.1186/s13287-020-01836-ymiR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathwayYuchen Li0Jinju Wang1Shuzhen Chen2Pei Wu3Shancai Xu4Chunlei Wang5Huaizhang Shi6Ji Bihl7Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State UniversityDepartment of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State UniversityDepartment of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State UniversityDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital, Harbin Medical UniversityDepartment of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State UniversityAbstract Background We have previously verified the beneficial effects of exosomes from endothelial progenitor cells (EPC-EXs) in ischemic stroke. However, the effects of EPC-EXs in hemorrhagic stroke have not been investigated. Additionally, miR-137 is reported to regulate ferroptosis and to be involved in the neuroprotection against ischemic stroke. Hence, the present work explored the effects of miR-137-overexpressing EPC-EXs on apoptosis, mitochondrial dysfunction, and ferroptosis in oxyhemoglobin (oxyHb)-injured SH-SY5Y cells. Methods The lentiviral miR-137 was transfected into EPCs and then the EPC-EXs were collected. RT-PCR was used to detect the miR-137 level in EPCs, EXs, and neurons. The uptake mechanisms of EPC-EXs in SH-SY5Y cells were explored by the co-incubation of Dynasore, Pitstop 2, Ly294002, and Genistein. After the transfection of different types of EPC-EXs, flow cytometry and expression of cytochrome c and cleaved caspase-3 were used to detect the apoptosis of oxyHb-injured neurons. Neuronal mitochondrial function was assessed by reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP) depolarization, and cellular ATP content. Cell ferroptosis was measured by lipid peroxidation, iron overload, degradation of glutathione, and glutathione peroxidase 4. Additionally, recombinational PGE2 was used to detect if activation of COX2/PGE2 pathway could reverse the protection of miR-137 overexpression. Results The present work showed (1) EPC-EXs could be taken in by SH-SY5Y cells via caveolin-/clathrin-mediated pathways and macropinocytosis; (2) miR-137 was decreased in neurons after oxyHb treatment, and EXsmiR-137 could restore the miR-137 levels; (3) EXsmiR-137 worked better than EXs in reducing the number of apoptotic neurons and pro-apoptotic protein expression after oxyHb treatment; (4) EXsmiR-137 are more effective in improving the cellular MMP, ROS, and ATP level; (5) EXsmiR-137, but not EXs, protected oxyHb-treated SH-SY5Y cells against lipid peroxidation, iron overload, degradation of glutathione, and glutathione peroxidase 4; and (6) EXsmiR-137 suppressed the expression of the COX2/PGE2 pathway, and activation of the pathway could partially reverse the neuroprotective effects of EXsmiR-137. Conclusion miR-137 overexpression boosts the neuroprotective effects of EPC-EXs against apoptosis and mitochondrial dysfunction in oxyHb-treated SH-SY5Y cells. Furthermore, EXsmiR-137 rather than EXs can restore the decrease in miR-137 levels and inhibit ferroptosis, and the protection mechanism might involve the miR-137-COX2/PGE2 signaling pathway.http://link.springer.com/article/10.1186/s13287-020-01836-ymiR-137OxyhemoglobinExosomesCOX2Ferroptosis |
| spellingShingle | Yuchen Li Jinju Wang Shuzhen Chen Pei Wu Shancai Xu Chunlei Wang Huaizhang Shi Ji Bihl miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway Stem Cell Research & Therapy miR-137 Oxyhemoglobin Exosomes COX2 Ferroptosis |
| title | miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway |
| title_full | miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway |
| title_fullStr | miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway |
| title_full_unstemmed | miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway |
| title_short | miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway |
| title_sort | mir 137 boosts the neuroprotective effect of endothelial progenitor cell derived exosomes in oxyhemoglobin treated sh sy5y cells partially via cox2 pge2 pathway |
| topic | miR-137 Oxyhemoglobin Exosomes COX2 Ferroptosis |
| url | http://link.springer.com/article/10.1186/s13287-020-01836-y |
| work_keys_str_mv | AT yuchenli mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT jinjuwang mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT shuzhenchen mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT peiwu mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT shancaixu mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT chunleiwang mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT huaizhangshi mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway AT jibihl mir137booststheneuroprotectiveeffectofendothelialprogenitorcellderivedexosomesinoxyhemoglobintreatedshsy5ycellspartiallyviacox2pge2pathway |