The Apis mellifera Filamentous Virus Genome
A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs),...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-07-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/7/2798 |
| _version_ | 1828758090138255360 |
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| author | Laurent Gauthier Scott Cornman Ulrike Hartmann François Cousserans Jay D. Evans Joachim R. de Miranda Peter Neumann |
| author_facet | Laurent Gauthier Scott Cornman Ulrike Hartmann François Cousserans Jay D. Evans Joachim R. de Miranda Peter Neumann |
| author_sort | Laurent Gauthier |
| collection | DOAJ |
| description | A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs), equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74) and BRO (Baculovirus Repeated Open Reading Frame). The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family. |
| first_indexed | 2024-12-11T00:36:45Z |
| format | Article |
| id | doaj.art-392867c7fcfa46e190c02a6d3f220127 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T00:36:45Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-392867c7fcfa46e190c02a6d3f2201272022-12-22T01:27:06ZengMDPI AGViruses1999-49152015-07-01773798381510.3390/v7072798v7072798The Apis mellifera Filamentous Virus GenomeLaurent Gauthier0Scott Cornman1Ulrike Hartmann2François Cousserans3Jay D. Evans4Joachim R. de Miranda5Peter Neumann6Agroscope, Swiss Bee Research Centre, Schwarzenburgstrasse 161, CH-3003 Bern, SwitzerlandBee Research Laboratory, Beltsville, MD 20705, USAAgroscope, Swiss Bee Research Centre, Schwarzenburgstrasse 161, CH-3003 Bern, SwitzerlandAgroscope, Swiss Bee Research Centre, Schwarzenburgstrasse 161, CH-3003 Bern, SwitzerlandBee Research Laboratory, Beltsville, MD 20705, USADepartment of Ecology, Swedish University of Agricultural Sciences, Uppsala 750 07, SwedenAgroscope, Swiss Bee Research Centre, Schwarzenburgstrasse 161, CH-3003 Bern, SwitzerlandA complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs), equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74) and BRO (Baculovirus Repeated Open Reading Frame). The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family.http://www.mdpi.com/1999-4915/7/7/2798Apis melliferafilamentous virusgenome annotation |
| spellingShingle | Laurent Gauthier Scott Cornman Ulrike Hartmann François Cousserans Jay D. Evans Joachim R. de Miranda Peter Neumann The Apis mellifera Filamentous Virus Genome Viruses Apis mellifera filamentous virus genome annotation |
| title | The Apis mellifera Filamentous Virus Genome |
| title_full | The Apis mellifera Filamentous Virus Genome |
| title_fullStr | The Apis mellifera Filamentous Virus Genome |
| title_full_unstemmed | The Apis mellifera Filamentous Virus Genome |
| title_short | The Apis mellifera Filamentous Virus Genome |
| title_sort | apis mellifera filamentous virus genome |
| topic | Apis mellifera filamentous virus genome annotation |
| url | http://www.mdpi.com/1999-4915/7/7/2798 |
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