Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections
ABSTRACT: Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-p...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521002915 |
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| author | S. Corbellini E. Scaltriti G. Piccinelli F. Gurrieri M. Mascherpa G. Boroni C. Amolini A. Caruso M.A. De Francesco |
| author_facet | S. Corbellini E. Scaltriti G. Piccinelli F. Gurrieri M. Mascherpa G. Boroni C. Amolini A. Caruso M.A. De Francesco |
| author_sort | S. Corbellini |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-producing blaNDM-5 and blaOXA-1. Methods: Three Escherichia coli isolates obtained from patients with invasive infections were analysed by phenotypic antibiotic susceptibility testing and whole-genome sequencing (WGS). Results: All of the isolates were resistant to carbapenems, most β-lactam antibiotics, piperacillin/tazobactam, amoxicillin/clavulanic acid and ciprofloxacin, remaining susceptible to amikacin, fosfomycin, colistin and tigecycline. The isolates belonged to sequence types ST44, ST405 and ST167 and co-harboured the blaNDM-5 and blaOXA-1 genes. Two of the isolates also harboured extended-spectrum β-lactamase (ESBL) genes (blaCTX-M-15 and blaTEM-1b). The blaNDM-5 gene was probably carried chromosomally even if different plasmids were identified. Various virulence genes were also identified. Conclusion: Our results highlight that continuous surveillance is essential to monitor the spread of clinically important MDR pathogens. |
| first_indexed | 2024-12-13T00:47:30Z |
| format | Article |
| id | doaj.art-392b496e8df84152826938f166c1d2c2 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-13T00:47:30Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-392b496e8df84152826938f166c1d2c22022-12-22T00:05:00ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-03-0128136139Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infectionsS. Corbellini0E. Scaltriti1G. Piccinelli2F. Gurrieri3M. Mascherpa4G. Boroni5C. Amolini6A. Caruso7M.A. De Francesco8Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, ItalyInstitute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Parma, ItalyInstitute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, ItalyInstitute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, ItalyDepartment of Obstetrics and Gynecology, University of Brescia–ASST Spedali Civili, Brescia, ItalyPediatric Surgery Unit, ASST Spedali Civili of Brescia, Brescia, ItalyMedicine 1 Unit Geriatric Section, Montichiari Hospital, ASST–Spedali Civili of Brescia, Brescia, ItalyInstitute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, ItalyInstitute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy; Corresponding author. Mailing address: Institute of Microbiology, Department of Molecular and Translational Medicine, P. le Spedali Civili 1, 25123 Brescia, Italy. Tel.: +39 030 399 5860; fax: +39 030 399 6071.ABSTRACT: Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-producing blaNDM-5 and blaOXA-1. Methods: Three Escherichia coli isolates obtained from patients with invasive infections were analysed by phenotypic antibiotic susceptibility testing and whole-genome sequencing (WGS). Results: All of the isolates were resistant to carbapenems, most β-lactam antibiotics, piperacillin/tazobactam, amoxicillin/clavulanic acid and ciprofloxacin, remaining susceptible to amikacin, fosfomycin, colistin and tigecycline. The isolates belonged to sequence types ST44, ST405 and ST167 and co-harboured the blaNDM-5 and blaOXA-1 genes. Two of the isolates also harboured extended-spectrum β-lactamase (ESBL) genes (blaCTX-M-15 and blaTEM-1b). The blaNDM-5 gene was probably carried chromosomally even if different plasmids were identified. Various virulence genes were also identified. Conclusion: Our results highlight that continuous surveillance is essential to monitor the spread of clinically important MDR pathogens.http://www.sciencedirect.com/science/article/pii/S2213716521002915β-LactamaseAntibiotic resistanceCarbapenem resistanceWhole-genome sequencingWGS |
| spellingShingle | S. Corbellini E. Scaltriti G. Piccinelli F. Gurrieri M. Mascherpa G. Boroni C. Amolini A. Caruso M.A. De Francesco Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections Journal of Global Antimicrobial Resistance β-Lactamase Antibiotic resistance Carbapenem resistance Whole-genome sequencing WGS |
| title | Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections |
| title_full | Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections |
| title_fullStr | Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections |
| title_full_unstemmed | Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections |
| title_short | Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections |
| title_sort | genomic characterisation of escherichia coli isolates co producing ndm 5 and oxa 1 from hospitalised patients with invasive infections |
| topic | β-Lactamase Antibiotic resistance Carbapenem resistance Whole-genome sequencing WGS |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521002915 |
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