Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes
Postbiotics, including bacterial lysates, are considered alternatives to probiotics. The aim of the current study was to investigate the effect of bacterial lysates (BLs) extracted from <i>Pediococcus acidilactici</i> K10 (K10 BL) and <i>P. acidilactici</i> HW01 (HW01 BL) on...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11614 |
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| author | Han Bin Lee Seok-Seong Kang |
| author_facet | Han Bin Lee Seok-Seong Kang |
| author_sort | Han Bin Lee |
| collection | DOAJ |
| description | Postbiotics, including bacterial lysates, are considered alternatives to probiotics. The aim of the current study was to investigate the effect of bacterial lysates (BLs) extracted from <i>Pediococcus acidilactici</i> K10 (K10 BL) and <i>P. acidilactici</i> HW01 (HW01 BL) on the differentiation of 3T3-L1 pre-adipocytes. Both K10 and HW01 BLs significantly reduced the accumulation of lipid droplets and the amounts of cellular glycerides in 3T3-L1 cells (<i>p</i> < 0.05). However, another postbiotic molecule, peptidoglycan of <i>P. acidilactici</i> K10 and <i>P. acidilactici</i> HW01, moderately inhibited the accumulation of lipid droplets, whereas heat-killed <i>P. acidilactici</i> did not effectively inhibit the lipid accumulation. The mRNA and protein levels of the transcription factors, peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α, responsible for the differentiation of 3T3-L1 cells, were significantly inhibited by K10 BL and HW01 BL (<i>p</i> < 0.05). Both K10 and HW01 BLs decreased adipocyte-related molecules, adipocyte fatty acid-binding protein and lipoprotein lipase, at the mRNA and protein levels. Furthermore, both K10 and HW01 BLs also downregulated the mRNA expression of leptin, but not resistin. Taken together, these results suggest that <i>P. acidilactici</i> BLs mediate anti-adipogenic effects by inhibiting adipogenic-related transcription factors and their target molecules. |
| first_indexed | 2024-03-09T21:38:59Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:38:59Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-393050b01495483b9663e066c3fd38fb2023-11-23T20:36:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191161410.3390/ijms231911614Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-AdipocytesHan Bin Lee0Seok-Seong Kang1Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang 10326, KoreaDepartment of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang 10326, KoreaPostbiotics, including bacterial lysates, are considered alternatives to probiotics. The aim of the current study was to investigate the effect of bacterial lysates (BLs) extracted from <i>Pediococcus acidilactici</i> K10 (K10 BL) and <i>P. acidilactici</i> HW01 (HW01 BL) on the differentiation of 3T3-L1 pre-adipocytes. Both K10 and HW01 BLs significantly reduced the accumulation of lipid droplets and the amounts of cellular glycerides in 3T3-L1 cells (<i>p</i> < 0.05). However, another postbiotic molecule, peptidoglycan of <i>P. acidilactici</i> K10 and <i>P. acidilactici</i> HW01, moderately inhibited the accumulation of lipid droplets, whereas heat-killed <i>P. acidilactici</i> did not effectively inhibit the lipid accumulation. The mRNA and protein levels of the transcription factors, peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α, responsible for the differentiation of 3T3-L1 cells, were significantly inhibited by K10 BL and HW01 BL (<i>p</i> < 0.05). Both K10 and HW01 BLs decreased adipocyte-related molecules, adipocyte fatty acid-binding protein and lipoprotein lipase, at the mRNA and protein levels. Furthermore, both K10 and HW01 BLs also downregulated the mRNA expression of leptin, but not resistin. Taken together, these results suggest that <i>P. acidilactici</i> BLs mediate anti-adipogenic effects by inhibiting adipogenic-related transcription factors and their target molecules.https://www.mdpi.com/1422-0067/23/19/11614postbioticsbacterial lysates<i>Pediococcus acidilactici</i>adipogenesis |
| spellingShingle | Han Bin Lee Seok-Seong Kang Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes International Journal of Molecular Sciences postbiotics bacterial lysates <i>Pediococcus acidilactici</i> adipogenesis |
| title | Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes |
| title_full | Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes |
| title_fullStr | Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes |
| title_full_unstemmed | Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes |
| title_short | Inhibitory Effect of Bacterial Lysates Extracted from <i>Pediococcus acidilactici</i> on the Differentiation of 3T3-L1 Pre-Adipocytes |
| title_sort | inhibitory effect of bacterial lysates extracted from i pediococcus acidilactici i on the differentiation of 3t3 l1 pre adipocytes |
| topic | postbiotics bacterial lysates <i>Pediococcus acidilactici</i> adipogenesis |
| url | https://www.mdpi.com/1422-0067/23/19/11614 |
| work_keys_str_mv | AT hanbinlee inhibitoryeffectofbacteriallysatesextractedfromipediococcusacidilacticiionthedifferentiationof3t3l1preadipocytes AT seokseongkang inhibitoryeffectofbacteriallysatesextractedfromipediococcusacidilacticiionthedifferentiationof3t3l1preadipocytes |