Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation
In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purifi...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2011-06-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018 |
| _version_ | 1811325710959116288 |
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| author | Christiane Trevisan Slivinski Alex Vinicius Lopes Machado Jorge Iulek Ricardo Antônio Ayub Mareci Mendes de Almeida |
| author_facet | Christiane Trevisan Slivinski Alex Vinicius Lopes Machado Jorge Iulek Ricardo Antônio Ayub Mareci Mendes de Almeida |
| author_sort | Christiane Trevisan Slivinski |
| collection | DOAJ |
| description | In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was inadvisable. K M and Vmax were 0.34 mg mL-1 and 160.22 U mL-1, respectively. |
| first_indexed | 2024-04-13T14:37:43Z |
| format | Article |
| id | doaj.art-393272619c3547fc8ef18249f44f298f |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-04-13T14:37:43Z |
| publishDate | 2011-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-393272619c3547fc8ef18249f44f298f2022-12-22T02:42:59ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242011-06-0154355956810.1590/S1516-89132011000300018Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentationChristiane Trevisan SlivinskiAlex Vinicius Lopes MachadoJorge IulekRicardo Antônio AyubMareci Mendes de AlmeidaIn this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was inadvisable. K M and Vmax were 0.34 mg mL-1 and 160.22 U mL-1, respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018glucoamylaseAspergillus nigersolid-state fermentationbiochemical characterizationpurification |
| spellingShingle | Christiane Trevisan Slivinski Alex Vinicius Lopes Machado Jorge Iulek Ricardo Antônio Ayub Mareci Mendes de Almeida Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation Brazilian Archives of Biology and Technology glucoamylase Aspergillus niger solid-state fermentation biochemical characterization purification |
| title | Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation |
| title_full | Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation |
| title_fullStr | Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation |
| title_full_unstemmed | Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation |
| title_short | Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation |
| title_sort | biochemical characterisation of a glucoamylase from aspergillus niger produced by solid state fermentation |
| topic | glucoamylase Aspergillus niger solid-state fermentation biochemical characterization purification |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018 |
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