Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems
Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing,...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/11/1138 |
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| author | Nuno R. da Silva Paula Jorge José A. Martins José A. Teixeira João C. Marcos |
| author_facet | Nuno R. da Silva Paula Jorge José A. Martins José A. Teixeira João C. Marcos |
| author_sort | Nuno R. da Silva |
| collection | DOAJ |
| description | Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA remains the key manufacturing step. Despite its high resolution, the scaling-up of chromatography is usually difficult and presents low capacity, resulting in low yields. Alternative methods that are based on aqueous two-phase systems (ATPSs) have been studied. Although higher yields may be obtained, its selectivity is often low. In this work, modified polymers based on poly(ethylene glycol) (PEG) derivatisation with amino groups (PEG–amine) or conjugation with positively charged amino acids (PEG–lysine, PEG–arginine, and PEG–histidine) were studied to increase the selectivity of PEG–dextran systems towards the partition of a model plasmid. A two-step strategy was employed to obtain suitable pure formulations of pDNA. In the first step, a PEG–dextran system with the addition of the affinity ligand was used with the recovery of the pDNA in the PEG-rich phase. Then, the pDNA was re-extracted to an ammonium-sulphate-rich phase in the second step. After removing the salt, this method yielded a purified preparation of pDNA without RNA and protein contamination. |
| first_indexed | 2024-03-10T05:20:33Z |
| format | Article |
| id | doaj.art-3933f99fe1864318b7a9707462897032 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T05:20:33Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-3933f99fe1864318b7a97074628970322023-11-23T00:03:22ZengMDPI AGLife2075-17292021-10-011111113810.3390/life11111138Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase SystemsNuno R. da Silva0Paula Jorge1José A. Martins2José A. Teixeira3João C. Marcos4Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Chemistry, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalGene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA remains the key manufacturing step. Despite its high resolution, the scaling-up of chromatography is usually difficult and presents low capacity, resulting in low yields. Alternative methods that are based on aqueous two-phase systems (ATPSs) have been studied. Although higher yields may be obtained, its selectivity is often low. In this work, modified polymers based on poly(ethylene glycol) (PEG) derivatisation with amino groups (PEG–amine) or conjugation with positively charged amino acids (PEG–lysine, PEG–arginine, and PEG–histidine) were studied to increase the selectivity of PEG–dextran systems towards the partition of a model plasmid. A two-step strategy was employed to obtain suitable pure formulations of pDNA. In the first step, a PEG–dextran system with the addition of the affinity ligand was used with the recovery of the pDNA in the PEG-rich phase. Then, the pDNA was re-extracted to an ammonium-sulphate-rich phase in the second step. After removing the salt, this method yielded a purified preparation of pDNA without RNA and protein contamination.https://www.mdpi.com/2075-1729/11/11/1138aqueous two-phase systemsaffinity partitionnon-viral vectorsplasmid DNA purificationgene therapyDNA vaccines |
| spellingShingle | Nuno R. da Silva Paula Jorge José A. Martins José A. Teixeira João C. Marcos Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems Life aqueous two-phase systems affinity partition non-viral vectors plasmid DNA purification gene therapy DNA vaccines |
| title | Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems |
| title_full | Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems |
| title_fullStr | Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems |
| title_full_unstemmed | Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems |
| title_short | Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems |
| title_sort | initial screening of poly ethylene glycol amino ligands for affinity purification of plasmid dna in aqueous two phase systems |
| topic | aqueous two-phase systems affinity partition non-viral vectors plasmid DNA purification gene therapy DNA vaccines |
| url | https://www.mdpi.com/2075-1729/11/11/1138 |
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