Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems

Initial Screening of Poly(ethylene glycol) Amino Ligands for Affinity Purification of Plasmid DNA in Aqueous Two-Phase Systems

Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing,...

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Bibliographic Details
Main Authors: Nuno R. da Silva, Paula Jorge, José A. Martins, José A. Teixeira, João C. Marcos
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Life
Subjects:
aqueous two-phase systems
affinity partition
non-viral vectors
plasmid DNA purification
gene therapy
DNA vaccines
Online Access:https://www.mdpi.com/2075-1729/11/11/1138

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