Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening.
<h4>Background</h4>Inhibition of apoptosis is one of the mechanisms selected by numerous intracellular pathogenic bacteria to control their host cell. Brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of th...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-10-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20949000/pdf/?tool=EBI |
| _version_ | 1831632809032155136 |
|---|---|
| author | Géraldine Laloux Michaël Deghelt Marie de Barsy Jean-Jacques Letesson Xavier De Bolle |
| author_facet | Géraldine Laloux Michaël Deghelt Marie de Barsy Jean-Jacques Letesson Xavier De Bolle |
| author_sort | Géraldine Laloux |
| collection | DOAJ |
| description | <h4>Background</h4>Inhibition of apoptosis is one of the mechanisms selected by numerous intracellular pathogenic bacteria to control their host cell. Brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche.<h4>Methodology/principal findings</h4>In order to identify Brucella melitensis anti-apoptotic effector candidates, we performed a genome-wide functional screening in yeast. The B. melitensis ORFeome was screened to identify inhibitors of Bax-induced cell death in S. cerevisiae. B. melitensis porin Omp2b, here shown to be essential, prevents Bax lethal effect in yeast, unlike its close paralog Omp2a. Our results based on Omp2b size variants characterization suggest that signal peptide processing is required for Omp2b effect in yeast.<h4>Conclusion/significance</h4>We report here the first application to a bacterial genome-wide library of coding sequences of this "yeast-rescue" screening strategy, previously used to highlight several new apoptosis regulators. Our work provides B. melitensis proteins that are candidates for an anti-apoptotic function, and can be tested in mammalian cells in the future. Hypotheses on possible molecular mechanisms of Bax inhibition by the B. melitensis porin Omp2b are discussed. |
| first_indexed | 2024-12-19T05:25:17Z |
| format | Article |
| id | doaj.art-3935be1d220446fd9333e573f7e902e3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T05:25:17Z |
| publishDate | 2010-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3935be1d220446fd9333e573f7e902e32022-12-21T20:34:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-10-01510e1327410.1371/journal.pone.0013274Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening.Géraldine LalouxMichaël DegheltMarie de BarsyJean-Jacques LetessonXavier De Bolle<h4>Background</h4>Inhibition of apoptosis is one of the mechanisms selected by numerous intracellular pathogenic bacteria to control their host cell. Brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche.<h4>Methodology/principal findings</h4>In order to identify Brucella melitensis anti-apoptotic effector candidates, we performed a genome-wide functional screening in yeast. The B. melitensis ORFeome was screened to identify inhibitors of Bax-induced cell death in S. cerevisiae. B. melitensis porin Omp2b, here shown to be essential, prevents Bax lethal effect in yeast, unlike its close paralog Omp2a. Our results based on Omp2b size variants characterization suggest that signal peptide processing is required for Omp2b effect in yeast.<h4>Conclusion/significance</h4>We report here the first application to a bacterial genome-wide library of coding sequences of this "yeast-rescue" screening strategy, previously used to highlight several new apoptosis regulators. Our work provides B. melitensis proteins that are candidates for an anti-apoptotic function, and can be tested in mammalian cells in the future. Hypotheses on possible molecular mechanisms of Bax inhibition by the B. melitensis porin Omp2b are discussed.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20949000/pdf/?tool=EBI |
| spellingShingle | Géraldine Laloux Michaël Deghelt Marie de Barsy Jean-Jacques Letesson Xavier De Bolle Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. PLoS ONE |
| title | Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. |
| title_full | Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. |
| title_fullStr | Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. |
| title_full_unstemmed | Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. |
| title_short | Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screening. |
| title_sort | identification of the essential brucella melitensis porin omp2b as a suppressor of bax induced cell death in yeast in a genome wide screening |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20949000/pdf/?tool=EBI |
| work_keys_str_mv | AT geraldinelaloux identificationoftheessentialbrucellamelitensisporinomp2basasuppressorofbaxinducedcelldeathinyeastinagenomewidescreening AT michaeldeghelt identificationoftheessentialbrucellamelitensisporinomp2basasuppressorofbaxinducedcelldeathinyeastinagenomewidescreening AT mariedebarsy identificationoftheessentialbrucellamelitensisporinomp2basasuppressorofbaxinducedcelldeathinyeastinagenomewidescreening AT jeanjacquesletesson identificationoftheessentialbrucellamelitensisporinomp2basasuppressorofbaxinducedcelldeathinyeastinagenomewidescreening AT xavierdebolle identificationoftheessentialbrucellamelitensisporinomp2basasuppressorofbaxinducedcelldeathinyeastinagenomewidescreening |