Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
Abstract While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull frac...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18643-5 |
| _version_ | 1798004351838453760 |
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| author | Larissa K. Dill Natalie A. Sims Ali Shad Chidozie Anyaegbu Andrew Warnock Yilin Mao Melinda Fitzgerald Bridgette D. Semple |
| author_facet | Larissa K. Dill Natalie A. Sims Ali Shad Chidozie Anyaegbu Andrew Warnock Yilin Mao Melinda Fitzgerald Bridgette D. Semple |
| author_sort | Larissa K. Dill |
| collection | DOAJ |
| description | Abstract While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull fractures. To address this, young adult female Piebald Viral Glaxo rats received sham, 1×, 2× or 3× closed-head mTBIs delivered at 24 h intervals, using a weight-drop device custom-built for reproducible impact. Skull bones were collected at 2 or 10 weeks after the final injury/sham procedure, imaged by micro computed tomography and analyzed at predetermined regions of interest. In the interparietal bone, proximal to the injury site, modest increases in bone thickness were observed at 2 weeks, particularly following 2× and 3× mTBI. By 10 weeks, 2× mTBI induced a robust increase in the volume and thickness of the interparietal bone, alongside a corresponding decrease in the volume of marrow cavities in the diploë region. In contrast, neither parietal nor frontal skull samples were affected by rmTBI. Our findings demonstrate time- and location-dependent effects of rmTBI on cranial bone structure, highlighting a need to consider microstructural alterations to cranial bone when assessing the consequences of rmTBI. |
| first_indexed | 2024-04-11T12:22:27Z |
| format | Article |
| id | doaj.art-39362a8ea7ce4d37b70c770ad3507d55 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T12:22:27Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-39362a8ea7ce4d37b70c770ad3507d552022-12-22T04:24:03ZengNature PortfolioScientific Reports2045-23222022-09-0112111110.1038/s41598-022-18643-5Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuriesLarissa K. Dill0Natalie A. Sims1Ali Shad2Chidozie Anyaegbu3Andrew Warnock4Yilin Mao5Melinda Fitzgerald6Bridgette D. Semple7Department of Neuroscience, Central Clinical School, Monash UniversitySt Vincent’s Institute of Medical ResearchDepartment of Neuroscience, Central Clinical School, Monash UniversityCurtin Health Innovation Research Institute, Faculty of Health SciencesCurtin Health Innovation Research Institute, Faculty of Health SciencesCurtin Health Innovation Research Institute, Faculty of Health SciencesCurtin Health Innovation Research Institute, Faculty of Health SciencesDepartment of Neuroscience, Central Clinical School, Monash UniversityAbstract While it is well-established that bone responds dynamically to mechanical loading, the effects of mild traumatic brain injury (mTBI) on cranial bone composition are unclear. We hypothesized that repeated mTBI (rmTBI) would change the microstructure of cranial bones, without gross skull fractures. To address this, young adult female Piebald Viral Glaxo rats received sham, 1×, 2× or 3× closed-head mTBIs delivered at 24 h intervals, using a weight-drop device custom-built for reproducible impact. Skull bones were collected at 2 or 10 weeks after the final injury/sham procedure, imaged by micro computed tomography and analyzed at predetermined regions of interest. In the interparietal bone, proximal to the injury site, modest increases in bone thickness were observed at 2 weeks, particularly following 2× and 3× mTBI. By 10 weeks, 2× mTBI induced a robust increase in the volume and thickness of the interparietal bone, alongside a corresponding decrease in the volume of marrow cavities in the diploë region. In contrast, neither parietal nor frontal skull samples were affected by rmTBI. Our findings demonstrate time- and location-dependent effects of rmTBI on cranial bone structure, highlighting a need to consider microstructural alterations to cranial bone when assessing the consequences of rmTBI.https://doi.org/10.1038/s41598-022-18643-5 |
| spellingShingle | Larissa K. Dill Natalie A. Sims Ali Shad Chidozie Anyaegbu Andrew Warnock Yilin Mao Melinda Fitzgerald Bridgette D. Semple Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries Scientific Reports |
| title | Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| title_full | Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| title_fullStr | Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| title_full_unstemmed | Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| title_short | Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| title_sort | localized time dependent responses of rat cranial bone to repeated mild traumatic brain injuries |
| url | https://doi.org/10.1038/s41598-022-18643-5 |
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