HIV RGB: Automated Single-Cell Analysis of HIV-1 Rev-Dependent RNA Nuclear Export and Translation Using Image Processing in KNIME

HIV RGB: Automated Single-Cell Analysis of HIV-1 Rev-Dependent RNA Nuclear Export and Translation Using Image Processing in KNIME

Single-cell imaging has emerged as a powerful means to study viral replication dynamics and identify sites of virus–host interactions. Multivariate aspects of viral replication cycles yield challenges inherent to handling large, complex imaging datasets. Herein, we describe the design and implementa...

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Bibliographic Details
Main Authors: Edward L. Evans, Ginger M. Pocock, Gabriel Einsdorf, Ryan T. Behrens, Ellen T. A. Dobson, Marcel Wiedenmann, Christian Birkhold, Paul Ahlquist, Kevin W. Eliceiri, Nathan M. Sherer
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Viruses
Subjects:
human immunodeficiency virus type 1
retrovirus
Rev
Rev response element
Gag
unspliced RNA
Online Access:https://www.mdpi.com/1999-4915/14/5/903

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