A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars
Knowledge of seasonal shifts in the bacterial community composition among different mulberry (Morus L.) cultivars will facilitate to develop the biocontrol phytopathogens strategy using endophytic bacteria. The present study investigated the endophytic bacterial communities of four mulberry cultivar...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-01-01
|
| Series: | Computational and Structural Biotechnology Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037019302223 |
| _version_ | 1828189143282221056 |
|---|---|
| author | Ting Ou Wei-fang Xu Fei Wang Gary Strobel Ze-yang Zhou Zhong-huai Xiang Jia Liu Jie Xie |
| author_facet | Ting Ou Wei-fang Xu Fei Wang Gary Strobel Ze-yang Zhou Zhong-huai Xiang Jia Liu Jie Xie |
| author_sort | Ting Ou |
| collection | DOAJ |
| description | Knowledge of seasonal shifts in the bacterial community composition among different mulberry (Morus L.) cultivars will facilitate to develop the biocontrol phytopathogens strategy using endophytic bacteria. The present study investigated the endophytic bacterial communities of four mulberry cultivars that have different resistance to mulberry fruit sclerotiniosis using Illumina-based sequencing of the 16S rRNA gene fragment in spring and autumn. The results indicated that spring samples harbor higher bacterial operational taxonomic units (OTUs), α-diversity, and bacterial community complexity in comparison with autumn samples. The taxonomic composition analysis showed that the majority of endophytes were composed of Proteobacteria (genus level: Methylobaterium) and Actinobacteria in spring, while sequences classified as Proteobacteria (genus level: Pantoea and Pseudomonas) were abundant in autumn. Analysis of β-diversity also revealed endophytic bacteria were divided into two main groups by season. By comparison among different mulberry cultivars, we found that Pantoea, Methylobaterium, and Pseudomonas were the three major bacterial genera in all cultivars, while their relative abundances varied with cultivars and appeared no obvious relationship with resistance level of mulberry fruit sclerotiniosis. The complex correlation of the endophytic communities in susceptible mulberry cultivars was higher than that of the resistant cultivars. Overall, the findings suggested that season plays a key role in determining the mulberry endophytic bacterial communities, followed by host cultivar, and Proteobacteria was the predominant phylum in both seasons and different mulberry cultivars. Keywords: Endophytic bacteria, Microbial diversity, Mulberry cultivar, Seasonal variation |
| first_indexed | 2024-04-12T08:01:54Z |
| format | Article |
| id | doaj.art-393afa1d026c42cfb6885cefdb7a87d2 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-04-12T08:01:54Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-393afa1d026c42cfb6885cefdb7a87d22022-12-22T03:41:18ZengElsevierComputational and Structural Biotechnology Journal2001-03702019-01-011710911100A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry CultivarsTing Ou0Wei-fang Xu1Fei Wang2Gary Strobel3Ze-yang Zhou4Zhong-huai Xiang5Jia Liu6Jie Xie7State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, ChinaDepartment of Plant Sciences, Montana State University, Bozeman, MT 59717, USAState Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, China; College of Life Science, Chongqing Normal University, Chongqing 400047, ChinaState Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, ChinaChongqing Key Laboratory of Economic Plant Biotechnology, College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402160, China; Corresponding author.State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, China; Corresponding author at: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China.Knowledge of seasonal shifts in the bacterial community composition among different mulberry (Morus L.) cultivars will facilitate to develop the biocontrol phytopathogens strategy using endophytic bacteria. The present study investigated the endophytic bacterial communities of four mulberry cultivars that have different resistance to mulberry fruit sclerotiniosis using Illumina-based sequencing of the 16S rRNA gene fragment in spring and autumn. The results indicated that spring samples harbor higher bacterial operational taxonomic units (OTUs), α-diversity, and bacterial community complexity in comparison with autumn samples. The taxonomic composition analysis showed that the majority of endophytes were composed of Proteobacteria (genus level: Methylobaterium) and Actinobacteria in spring, while sequences classified as Proteobacteria (genus level: Pantoea and Pseudomonas) were abundant in autumn. Analysis of β-diversity also revealed endophytic bacteria were divided into two main groups by season. By comparison among different mulberry cultivars, we found that Pantoea, Methylobaterium, and Pseudomonas were the three major bacterial genera in all cultivars, while their relative abundances varied with cultivars and appeared no obvious relationship with resistance level of mulberry fruit sclerotiniosis. The complex correlation of the endophytic communities in susceptible mulberry cultivars was higher than that of the resistant cultivars. Overall, the findings suggested that season plays a key role in determining the mulberry endophytic bacterial communities, followed by host cultivar, and Proteobacteria was the predominant phylum in both seasons and different mulberry cultivars. Keywords: Endophytic bacteria, Microbial diversity, Mulberry cultivar, Seasonal variationhttp://www.sciencedirect.com/science/article/pii/S2001037019302223 |
| spellingShingle | Ting Ou Wei-fang Xu Fei Wang Gary Strobel Ze-yang Zhou Zhong-huai Xiang Jia Liu Jie Xie A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars Computational and Structural Biotechnology Journal |
| title | A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars |
| title_full | A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars |
| title_fullStr | A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars |
| title_full_unstemmed | A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars |
| title_short | A Microbiome Study Reveals Seasonal Variation in Endophytic Bacteria Among different Mulberry Cultivars |
| title_sort | microbiome study reveals seasonal variation in endophytic bacteria among different mulberry cultivars |
| url | http://www.sciencedirect.com/science/article/pii/S2001037019302223 |
| work_keys_str_mv | AT tingou amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT weifangxu amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT feiwang amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT garystrobel amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT zeyangzhou amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT zhonghuaixiang amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT jialiu amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT jiexie amicrobiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT tingou microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT weifangxu microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT feiwang microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT garystrobel microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT zeyangzhou microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT zhonghuaixiang microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT jialiu microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars AT jiexie microbiomestudyrevealsseasonalvariationinendophyticbacteriaamongdifferentmulberrycultivars |