Sequencing by hybridization of long targets.
Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences cau...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22574124/?tool=EBI |
| _version_ | 1818460839552745472 |
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| author | Yu Qin Tobias M Schneider Michael P Brenner |
| author_facet | Yu Qin Tobias M Schneider Michael P Brenner |
| author_sort | Yu Qin |
| collection | DOAJ |
| description | Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error. |
| first_indexed | 2024-12-14T23:36:38Z |
| format | Article |
| id | doaj.art-393b0e706b0b42b8ac0c11447bc0fc4d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T23:36:38Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-393b0e706b0b42b8ac0c11447bc0fc4d2022-12-21T22:43:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3581910.1371/journal.pone.0035819Sequencing by hybridization of long targets.Yu QinTobias M SchneiderMichael P BrennerSequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22574124/?tool=EBI |
| spellingShingle | Yu Qin Tobias M Schneider Michael P Brenner Sequencing by hybridization of long targets. PLoS ONE |
| title | Sequencing by hybridization of long targets. |
| title_full | Sequencing by hybridization of long targets. |
| title_fullStr | Sequencing by hybridization of long targets. |
| title_full_unstemmed | Sequencing by hybridization of long targets. |
| title_short | Sequencing by hybridization of long targets. |
| title_sort | sequencing by hybridization of long targets |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22574124/?tool=EBI |
| work_keys_str_mv | AT yuqin sequencingbyhybridizationoflongtargets AT tobiasmschneider sequencingbyhybridizationoflongtargets AT michaelpbrenner sequencingbyhybridizationoflongtargets |