Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma
Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/5/1/16 |
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| author | Natalia Shved Anna Egorova Natalia Osinovskaya Anton Kiselev |
| author_facet | Natalia Shved Anna Egorova Natalia Osinovskaya Anton Kiselev |
| author_sort | Natalia Shved |
| collection | DOAJ |
| description | Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in <i>MED12</i> and <i>HMGA2</i> genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested. |
| first_indexed | 2024-03-09T21:19:37Z |
| format | Article |
| id | doaj.art-393bc9af504f4004b164838e855ca9ea |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-09T21:19:37Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-393bc9af504f4004b164838e855ca9ea2023-11-23T21:24:31ZengMDPI AGMethods and Protocols2409-92792022-02-01511610.3390/mps5010016Development of Primary Monolayer Cell Model and Organotypic Model of Uterine LeiomyomaNatalia Shved0Anna Egorova1Natalia Osinovskaya2Anton Kiselev3Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, RussiaDepartment of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, RussiaCellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in <i>MED12</i> and <i>HMGA2</i> genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested.https://www.mdpi.com/2409-9279/5/1/16uterine leiomyomacell modelorganotypic model<i>MED12</i><i>HMGA2</i> |
| spellingShingle | Natalia Shved Anna Egorova Natalia Osinovskaya Anton Kiselev Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma Methods and Protocols uterine leiomyoma cell model organotypic model <i>MED12</i> <i>HMGA2</i> |
| title | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
| title_full | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
| title_fullStr | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
| title_full_unstemmed | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
| title_short | Development of Primary Monolayer Cell Model and Organotypic Model of Uterine Leiomyoma |
| title_sort | development of primary monolayer cell model and organotypic model of uterine leiomyoma |
| topic | uterine leiomyoma cell model organotypic model <i>MED12</i> <i>HMGA2</i> |
| url | https://www.mdpi.com/2409-9279/5/1/16 |
| work_keys_str_mv | AT nataliashved developmentofprimarymonolayercellmodelandorganotypicmodelofuterineleiomyoma AT annaegorova developmentofprimarymonolayercellmodelandorganotypicmodelofuterineleiomyoma AT nataliaosinovskaya developmentofprimarymonolayercellmodelandorganotypicmodelofuterineleiomyoma AT antonkiselev developmentofprimarymonolayercellmodelandorganotypicmodelofuterineleiomyoma |