Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells
Background Large‐conductance calcium‐ and voltage‐activated potassium channels (BKCa channels) play important roles in the maintenance of vascular tone, and their dysregulation is associated with abnormal vascular relaxation and contraction. We tested the changes in BKCa channel properties in patien...
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| Format: | Article |
| Language: | English |
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Wiley
2016-09-01
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| Series: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1161/JAHA.116.003913 |
| _version_ | 1819213480687828992 |
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| author | Jun Cheng Liang Mao Jing Wen Peng‐Yun Li Na Wang Xiao‐Qiu Tan Xiao‐Dong Zhang Xiao‐Rong Zeng Liang Xu Xian‐Ming Xia Dong Xia Kai He Song Su Hui Yao Yan Yang |
| author_facet | Jun Cheng Liang Mao Jing Wen Peng‐Yun Li Na Wang Xiao‐Qiu Tan Xiao‐Dong Zhang Xiao‐Rong Zeng Liang Xu Xian‐Ming Xia Dong Xia Kai He Song Su Hui Yao Yan Yang |
| author_sort | Jun Cheng |
| collection | DOAJ |
| description | Background Large‐conductance calcium‐ and voltage‐activated potassium channels (BKCa channels) play important roles in the maintenance of vascular tone, and their dysregulation is associated with abnormal vascular relaxation and contraction. We tested the changes in BKCa channel properties in patients at different ages to assess the effects of hypertension and aging on the functional changes of BKCa channels. Methods and Results Patch clamp was performed to detect the activities of BKCa channels in freshly isolated human mesenteric artery smooth muscle cells from younger patients (aged ≤45 years) without hypertension, older patients (aged ≥65 years) without hypertension, and older patients with hypertension. The expression of mRNA and protein from BKCa channels was evaluated by reverse transcription polymerase chain reaction and Western blot analysis, respectively. Results showed that the whole‐cell current density, spontaneous transient outward current, and Ca2+ sensitivity of the artery smooth muscle cells were significantly decreased in the older patients with hypertension; the decreases were insignificant in the older patients without hypertension, although a clear tendency to have spontaneous transient outward current was detected in these patients. The expression of both mRNA and protein of BKCa subunits α and β1 was significantly decreased in the older patients with hypertension but not in the older patients without hypertension compared with the younger patients without hypertension. Conclusions Our findings demonstrate for the first time that hypertension is an important factor for the pathological alteration of the properties of BKCa channels in human mesenteric artery smooth muscle cells, and aging itself may also be a factor in these changes in the cells. |
| first_indexed | 2024-12-23T06:59:32Z |
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| language | English |
| last_indexed | 2024-12-23T06:59:32Z |
| publishDate | 2016-09-01 |
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| series | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| spelling | doaj.art-393e505f1c9b42fcb23be9763da3df042022-12-21T17:56:14ZengWileyJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease2047-99802016-09-0159n/an/a10.1161/JAHA.116.003913Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle CellsJun Cheng0Liang Mao1Jing Wen2Peng‐Yun Li3Na Wang4Xiao‐Qiu Tan5Xiao‐Dong Zhang6Xiao‐Rong Zeng7Liang Xu8Xian‐Ming Xia9Dong Xia10Kai He11Song Su12Hui Yao13Yan Yang14Key Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaThe First Affiliated Hospital Southwest Medical University Luzhou ChinaKey Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease Institute of Cardiovascular Research Southwest Medical University Luzhou ChinaBackground Large‐conductance calcium‐ and voltage‐activated potassium channels (BKCa channels) play important roles in the maintenance of vascular tone, and their dysregulation is associated with abnormal vascular relaxation and contraction. We tested the changes in BKCa channel properties in patients at different ages to assess the effects of hypertension and aging on the functional changes of BKCa channels. Methods and Results Patch clamp was performed to detect the activities of BKCa channels in freshly isolated human mesenteric artery smooth muscle cells from younger patients (aged ≤45 years) without hypertension, older patients (aged ≥65 years) without hypertension, and older patients with hypertension. The expression of mRNA and protein from BKCa channels was evaluated by reverse transcription polymerase chain reaction and Western blot analysis, respectively. Results showed that the whole‐cell current density, spontaneous transient outward current, and Ca2+ sensitivity of the artery smooth muscle cells were significantly decreased in the older patients with hypertension; the decreases were insignificant in the older patients without hypertension, although a clear tendency to have spontaneous transient outward current was detected in these patients. The expression of both mRNA and protein of BKCa subunits α and β1 was significantly decreased in the older patients with hypertension but not in the older patients without hypertension compared with the younger patients without hypertension. Conclusions Our findings demonstrate for the first time that hypertension is an important factor for the pathological alteration of the properties of BKCa channels in human mesenteric artery smooth muscle cells, and aging itself may also be a factor in these changes in the cells.https://doi.org/10.1161/JAHA.116.003913ageBKCa channelshuman mesentery artery smooth muscle cellshypertensionpatch clamp |
| spellingShingle | Jun Cheng Liang Mao Jing Wen Peng‐Yun Li Na Wang Xiao‐Qiu Tan Xiao‐Dong Zhang Xiao‐Rong Zeng Liang Xu Xian‐Ming Xia Dong Xia Kai He Song Su Hui Yao Yan Yang Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease age BKCa channels human mesentery artery smooth muscle cells hypertension patch clamp |
| title | Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells |
| title_full | Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells |
| title_fullStr | Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells |
| title_full_unstemmed | Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells |
| title_short | Different Effects of Hypertension and Age on the Function of Large Conductance Calcium‐ and Voltage‐Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells |
| title_sort | different effects of hypertension and age on the function of large conductance calcium and voltage activated potassium channels in human mesentery artery smooth muscle cells |
| topic | age BKCa channels human mesentery artery smooth muscle cells hypertension patch clamp |
| url | https://doi.org/10.1161/JAHA.116.003913 |
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