DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head
Abstract Background Dysregulation of the OPG/RANK/RANKL signalling pathway is a key step in the occurrence of steroid-induced osteonecrosis of the femoral head (ONFH). This study aims to understand the degree of methylation of the OPG, RANK, and RANKL genes in steroid-related ONFH. Methods A case-co...
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| Format: | Article |
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BMC
2021-06-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-021-04472-6 |
| _version_ | 1818656215310270464 |
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| author | Menghu Sun Yuju Cao Xiaolong Yang Feimeng An Huiqiang Wu Jianzhong Wang |
| author_facet | Menghu Sun Yuju Cao Xiaolong Yang Feimeng An Huiqiang Wu Jianzhong Wang |
| author_sort | Menghu Sun |
| collection | DOAJ |
| description | Abstract Background Dysregulation of the OPG/RANK/RANKL signalling pathway is a key step in the occurrence of steroid-induced osteonecrosis of the femoral head (ONFH). This study aims to understand the degree of methylation of the OPG, RANK, and RANKL genes in steroid-related ONFH. Methods A case-control study was designed, including 50 patients (25 males and 25 females) and 50 matched controls. The European Molecular Biology Open Software Suite (EMBOSS) was used to predict the existence and location of CpG islands in the OPG, RANK, and RANKL genes. The Agena MassARRAY platform was used to detect the methylation status of the above genes in the blood of subjects. The relationship between the methylation level of CpG sites in each gene and steroid-related ONFH was analysed by the chi-square test, logistic regression analysis, and other statistical methods. Results In the CpG islands of the OPG, RANK, and RANKL genes in patients with steroid-related ONFH, several CpG sites with high methylation rates and high methylation levels were found. Some hypermethylated CpG sites increase the risk of steroid-related ONFH. In addition, a few hypermethylated CpG sites have predictive value for the early diagnosis of steroid-related ONFH. Conclusion Methylation of certain sites in the OPG/RANK/RANKL signalling pathway increases the risk of steroid-related ONFH. Some hypermethylated CpG sites may be used as early prediction and diagnostic targets for steroid-related ONFH. |
| first_indexed | 2024-12-17T03:22:02Z |
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| id | doaj.art-3941292c23654e3f9fd1fb9da6c9c5b4 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-17T03:22:02Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-3941292c23654e3f9fd1fb9da6c9c5b42022-12-21T22:05:30ZengBMCBMC Musculoskeletal Disorders1471-24742021-06-0122111210.1186/s12891-021-04472-6DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral headMenghu Sun0Yuju Cao1Xiaolong Yang2Feimeng An3Huiqiang Wu4Jianzhong Wang5Department of Orthopedics and Traumatology, The Second Affiliated Hospital of Inner Mongolia Medical UniversityZhengzhou Traditional Chinese Medicine (TCM) Traumatology HospitalDepartment of Orthopedics and Traumatology, The Second Affiliated Hospital of Inner Mongolia Medical UniversityInner Mongolia Autonomous Region Hospital of Traditional Chinese MedicineInner Mongolia Autonomous Region Hospital of Traditional Chinese MedicineDepartment of Orthopedics and Traumatology, The Second Affiliated Hospital of Inner Mongolia Medical UniversityAbstract Background Dysregulation of the OPG/RANK/RANKL signalling pathway is a key step in the occurrence of steroid-induced osteonecrosis of the femoral head (ONFH). This study aims to understand the degree of methylation of the OPG, RANK, and RANKL genes in steroid-related ONFH. Methods A case-control study was designed, including 50 patients (25 males and 25 females) and 50 matched controls. The European Molecular Biology Open Software Suite (EMBOSS) was used to predict the existence and location of CpG islands in the OPG, RANK, and RANKL genes. The Agena MassARRAY platform was used to detect the methylation status of the above genes in the blood of subjects. The relationship between the methylation level of CpG sites in each gene and steroid-related ONFH was analysed by the chi-square test, logistic regression analysis, and other statistical methods. Results In the CpG islands of the OPG, RANK, and RANKL genes in patients with steroid-related ONFH, several CpG sites with high methylation rates and high methylation levels were found. Some hypermethylated CpG sites increase the risk of steroid-related ONFH. In addition, a few hypermethylated CpG sites have predictive value for the early diagnosis of steroid-related ONFH. Conclusion Methylation of certain sites in the OPG/RANK/RANKL signalling pathway increases the risk of steroid-related ONFH. Some hypermethylated CpG sites may be used as early prediction and diagnostic targets for steroid-related ONFH.https://doi.org/10.1186/s12891-021-04472-6OPGRANKRANKLMethylationSteroid-induced ONFH |
| spellingShingle | Menghu Sun Yuju Cao Xiaolong Yang Feimeng An Huiqiang Wu Jianzhong Wang DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head BMC Musculoskeletal Disorders OPG RANK RANKL Methylation Steroid-induced ONFH |
| title | DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head |
| title_full | DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head |
| title_fullStr | DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head |
| title_full_unstemmed | DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head |
| title_short | DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head |
| title_sort | dna methylation in the opg rank rankl pathway is associated with steroid induced osteonecrosis of the femoral head |
| topic | OPG RANK RANKL Methylation Steroid-induced ONFH |
| url | https://doi.org/10.1186/s12891-021-04472-6 |
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