Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain
Abstract In our former studies based on a human whole-blood model infected with trans-anethole (TA)-treated Staphylococcus aureus Newman strain, we have observed that selected parameters/mechanisms of innate and acquired immune response were more enhanced in comparison to samples infected with non-t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34138-3 |
| _version_ | 1827956731105247232 |
|---|---|
| author | Paweł Kwiatkowski Karolina Rogulska Agata Pruss Monika Sienkiewicz Barbara Dołęgowska Iwona Wojciechowska-Koszko |
| author_facet | Paweł Kwiatkowski Karolina Rogulska Agata Pruss Monika Sienkiewicz Barbara Dołęgowska Iwona Wojciechowska-Koszko |
| author_sort | Paweł Kwiatkowski |
| collection | DOAJ |
| description | Abstract In our former studies based on a human whole-blood model infected with trans-anethole (TA)-treated Staphylococcus aureus Newman strain, we have observed that selected parameters/mechanisms of innate and acquired immune response were more enhanced in comparison to samples infected with non-treated bacteria. Due to this observation, the current study aimed to evaluate the concentration of selected proteins involved in both types of responses (IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-17, TNF-α, IFN-γ, G-CSF, C5a, CCL1—CCL5, CXCL1, CXCL2, CXCL9—CXCL11, MMP-8, TLR2, and PGLYRP1) in healthy participants' plasma after blood stimulation of TA-treated S. aureus Newman strain. Determination of analyzed protein concentration was conducted using Luminex and ELISA assays. Based on the results, it has been proven that the immunomodulatory potential of TA-treated S. aureus Newman strain on increasing IL-1β, IL-6, TNF-α, IL-12, G-CSF, C5a, CCL2—CCL4, CXCL1, CXCL2, MMP-8 and PGLYRP1 levels in plasma. Moreover, it has been also demonstrated an association between TNF-α and CCL4 in a blood model infected with TA-treated cells. More research is warranted to find more underlying mechanisms involved in the effects of TA-treated S. aureus Newman in human blood, mainly whether the observed “immunity boost” can be regulated after bacteria elimination. Therefore, the potential of TA should be further explored to understand under which conditions it might help treat or prevent infections caused by S. aureus. |
| first_indexed | 2024-04-09T15:09:39Z |
| format | Article |
| id | doaj.art-394187c2e636476d87b2ee3841eec936 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T15:09:39Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-394187c2e636476d87b2ee3841eec9362023-04-30T11:17:06ZengNature PortfolioScientific Reports2045-23222023-04-0113111010.1038/s41598-023-34138-3Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strainPaweł Kwiatkowski0Karolina Rogulska1Agata Pruss2Monika Sienkiewicz3Barbara Dołęgowska4Iwona Wojciechowska-Koszko5Department of Diagnostic Immunology, Pomeranian Medical University in SzczecinDepartment of Diagnostic Immunology, Pomeranian Medical University in SzczecinDepartment of Laboratory Medicine, Pomeranian Medical University in SzczecinDepartment of Pharmaceutical Microbiology and Microbiological Diagnostic, Medical University of LodzDepartment of Laboratory Medicine, Pomeranian Medical University in SzczecinDepartment of Diagnostic Immunology, Pomeranian Medical University in SzczecinAbstract In our former studies based on a human whole-blood model infected with trans-anethole (TA)-treated Staphylococcus aureus Newman strain, we have observed that selected parameters/mechanisms of innate and acquired immune response were more enhanced in comparison to samples infected with non-treated bacteria. Due to this observation, the current study aimed to evaluate the concentration of selected proteins involved in both types of responses (IL-1α, IL-1β, IL-2, IL-6, IL-12, IL-17, TNF-α, IFN-γ, G-CSF, C5a, CCL1—CCL5, CXCL1, CXCL2, CXCL9—CXCL11, MMP-8, TLR2, and PGLYRP1) in healthy participants' plasma after blood stimulation of TA-treated S. aureus Newman strain. Determination of analyzed protein concentration was conducted using Luminex and ELISA assays. Based on the results, it has been proven that the immunomodulatory potential of TA-treated S. aureus Newman strain on increasing IL-1β, IL-6, TNF-α, IL-12, G-CSF, C5a, CCL2—CCL4, CXCL1, CXCL2, MMP-8 and PGLYRP1 levels in plasma. Moreover, it has been also demonstrated an association between TNF-α and CCL4 in a blood model infected with TA-treated cells. More research is warranted to find more underlying mechanisms involved in the effects of TA-treated S. aureus Newman in human blood, mainly whether the observed “immunity boost” can be regulated after bacteria elimination. Therefore, the potential of TA should be further explored to understand under which conditions it might help treat or prevent infections caused by S. aureus.https://doi.org/10.1038/s41598-023-34138-3 |
| spellingShingle | Paweł Kwiatkowski Karolina Rogulska Agata Pruss Monika Sienkiewicz Barbara Dołęgowska Iwona Wojciechowska-Koszko Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain Scientific Reports |
| title | Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain |
| title_full | Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain |
| title_fullStr | Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain |
| title_full_unstemmed | Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain |
| title_short | Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain |
| title_sort | immunomodulatory effects of trans anethole treated staphylococcus aureus newman strain |
| url | https://doi.org/10.1038/s41598-023-34138-3 |
| work_keys_str_mv | AT pawełkwiatkowski immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain AT karolinarogulska immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain AT agatapruss immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain AT monikasienkiewicz immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain AT barbaradołegowska immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain AT iwonawojciechowskakoszko immunomodulatoryeffectsoftransanetholetreatedstaphylococcusaureusnewmanstrain |