Successful <i>i</i>-GONAD in Mice at Early Zygote Stage through In Vivo Electroporation Three Min after Intraoviductal Instillation of CRISPR-Ribonucleoprotein

Successful <i>i</i>-GONAD in Mice at Early Zygote Stage through In Vivo Electroporation Three Min after Intraoviductal Instillation of CRISPR-Ribonucleoprotein

Improved genome editing via oviductal nucleic acids delivery (<i>i</i>-GONAD) is a new technology enabling in situ genome editing of mammalian zygotes exiting the oviductal lumen, which is now available in mice, rats, and hamsters. In this method, CRISPR/Cas9 genome-editing reagents are...

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Bibliographic Details
Main Authors: Shuji Takabayashi, Kenta Iijima, Masumi Tsujimura, Takuya Aoshima, Hisayoshi Takagi, Kazushi Aoto, Masahiro Sato
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:International Journal of Molecular Sciences
Subjects:
<i>i</i>-GONAD
in vivo electroporation
genome editing
early zygotes
oviduct
cumulus cells
Online Access:https://www.mdpi.com/1422-0067/23/18/10678

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