Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model
Night-shift work is linked to a shift in food intake toward the normal sleeping period, and to metabolic disturbance. We applied a rat model of night-shift work to assess the immediate effects of such a shift in food intake on metabolism. Male Wistar rats were subjected to 8 h of forced activity dur...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-11-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6643/8/11/712 |
| _version_ | 1811230889263235072 |
|---|---|
| author | Andrea Rørvik Marti Peter Meerlo Janne Grønli Sjoerd Johan van Hasselt Jelena Mrdalj Ståle Pallesen Torhild Thue Pedersen Tone Elise Gjøtterud Henriksen Silje Skrede |
| author_facet | Andrea Rørvik Marti Peter Meerlo Janne Grønli Sjoerd Johan van Hasselt Jelena Mrdalj Ståle Pallesen Torhild Thue Pedersen Tone Elise Gjøtterud Henriksen Silje Skrede |
| author_sort | Andrea Rørvik Marti |
| collection | DOAJ |
| description | Night-shift work is linked to a shift in food intake toward the normal sleeping period, and to metabolic disturbance. We applied a rat model of night-shift work to assess the immediate effects of such a shift in food intake on metabolism. Male Wistar rats were subjected to 8 h of forced activity during their rest (ZT2-10) or active (ZT14-22) phase. Food intake, body weight, and body temperature were monitored across four work days and eight recovery days. Food intake gradually shifted toward rest-work hours, stabilizing on work day three. A subgroup of animals was euthanized after the third work session for analysis of metabolic gene expression in the liver by real-time polymerase chain reaction (PCR). Results show that work in the rest phase shifted food intake to rest-work hours. Moreover, liver genes related to energy storage and insulin metabolism were upregulated, and genes related to energy breakdown were downregulated compared to non-working time-matched controls. Both working groups lost weight during the protocol and regained weight during recovery, but animals that worked in the rest phase did not fully recover, even after eight days of recovery. In conclusion, three to four days of work in the rest phase is sufficient to induce disruption of several metabolic parameters, which requires more than eight days for full recovery. |
| first_indexed | 2024-04-12T10:35:42Z |
| format | Article |
| id | doaj.art-39432bf0d1b041078dd0e7c421ddb44f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-12T10:35:42Z |
| publishDate | 2016-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-39432bf0d1b041078dd0e7c421ddb44f2022-12-22T03:36:43ZengMDPI AGNutrients2072-66432016-11-0181171210.3390/nu8110712nu8110712Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat ModelAndrea Rørvik Marti0Peter Meerlo1Janne Grønli2Sjoerd Johan van Hasselt3Jelena Mrdalj4Ståle Pallesen5Torhild Thue Pedersen6Tone Elise Gjøtterud Henriksen7Silje Skrede8Department of Biological and Medical Psychology, University of Bergen, Bergen 5009, NorwayGroningen Institute for Evolutionary Life Sciences, University of Groningen, 9700 CC Groningen, The NetherlandsDepartment of Biological and Medical Psychology, University of Bergen, Bergen 5009, NorwayGroningen Institute for Evolutionary Life Sciences, University of Groningen, 9700 CC Groningen, The NetherlandsDepartment of Biological and Medical Psychology, University of Bergen, Bergen 5009, NorwayDepartment of Psychosocial Science, University of Bergen, Bergen 5015, NorwayDepartment of Biological and Medical Psychology, University of Bergen, Bergen 5009, NorwaySection of Psychiatry, Department of Clinical Medicine, Faculty of Medicine and Dentistry, University of Bergen, Bergen 5021, NorwayDr. Einar Martens Research Group for Biological Psychiatry, Center for Medical Genetics and Molecular Medicine, Haukeland Univeristy Hospital, 5021 Bergen, NorwayNight-shift work is linked to a shift in food intake toward the normal sleeping period, and to metabolic disturbance. We applied a rat model of night-shift work to assess the immediate effects of such a shift in food intake on metabolism. Male Wistar rats were subjected to 8 h of forced activity during their rest (ZT2-10) or active (ZT14-22) phase. Food intake, body weight, and body temperature were monitored across four work days and eight recovery days. Food intake gradually shifted toward rest-work hours, stabilizing on work day three. A subgroup of animals was euthanized after the third work session for analysis of metabolic gene expression in the liver by real-time polymerase chain reaction (PCR). Results show that work in the rest phase shifted food intake to rest-work hours. Moreover, liver genes related to energy storage and insulin metabolism were upregulated, and genes related to energy breakdown were downregulated compared to non-working time-matched controls. Both working groups lost weight during the protocol and regained weight during recovery, but animals that worked in the rest phase did not fully recover, even after eight days of recovery. In conclusion, three to four days of work in the rest phase is sufficient to induce disruption of several metabolic parameters, which requires more than eight days for full recovery.http://www.mdpi.com/2072-6643/8/11/712shift worknight workanimal modelmetabolismcircadian rhythmicitygene expressionbody temperaturebody weightfood intake |
| spellingShingle | Andrea Rørvik Marti Peter Meerlo Janne Grønli Sjoerd Johan van Hasselt Jelena Mrdalj Ståle Pallesen Torhild Thue Pedersen Tone Elise Gjøtterud Henriksen Silje Skrede Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model Nutrients shift work night work animal model metabolism circadian rhythmicity gene expression body temperature body weight food intake |
| title | Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model |
| title_full | Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model |
| title_fullStr | Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model |
| title_full_unstemmed | Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model |
| title_short | Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model |
| title_sort | shift in food intake and changes in metabolic regulation and gene expression during simulated night shift work a rat model |
| topic | shift work night work animal model metabolism circadian rhythmicity gene expression body temperature body weight food intake |
| url | http://www.mdpi.com/2072-6643/8/11/712 |
| work_keys_str_mv | AT andrearørvikmarti shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT petermeerlo shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT jannegrønli shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT sjoerdjohanvanhasselt shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT jelenamrdalj shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT stalepallesen shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT torhildthuepedersen shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT toneelisegjøtterudhenriksen shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel AT siljeskrede shiftinfoodintakeandchangesinmetabolicregulationandgeneexpressionduringsimulatednightshiftworkaratmodel |