β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice
The earliest interaction between macrophages and Paracoccidioides brasiliensis is particularly important in paracoccidioidomycosis (PCM) progression, and surface proteins play a central role in this process. The present study investigated the contribution of β2 integrin in P. brasiliensis-macrophage...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.622899/full |
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| author | Stephan Alberto Machado de Oliveira Stephan Alberto Machado de Oliveira Janayna Nunes Reis Elisa Catão Andre Correa Amaral Ana Camila Oliveira Souza Alice Melo Ribeiro Lúcia Helena Faccioli Fabiana Pirani Carneiro Clara Luna Freitas Marina Pedro Henrique Bürgel Larissa Fernandes Aldo Henrique Tavares Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca |
| author_facet | Stephan Alberto Machado de Oliveira Stephan Alberto Machado de Oliveira Janayna Nunes Reis Elisa Catão Andre Correa Amaral Ana Camila Oliveira Souza Alice Melo Ribeiro Lúcia Helena Faccioli Fabiana Pirani Carneiro Clara Luna Freitas Marina Pedro Henrique Bürgel Larissa Fernandes Aldo Henrique Tavares Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca |
| author_sort | Stephan Alberto Machado de Oliveira |
| collection | DOAJ |
| description | The earliest interaction between macrophages and Paracoccidioides brasiliensis is particularly important in paracoccidioidomycosis (PCM) progression, and surface proteins play a central role in this process. The present study investigated the contribution of β2 integrin in P. brasiliensis-macrophage interaction and PCM progression. We infected β2-low expression (CD18low) and wild type (WT) mice with P. brasiliensis 18. Disease progression was evaluated for fungal burden, lung granulomatous lesions, nitrate levels, and serum antibody production. Besides, the in vitro capacity of macrophages to internalize and kill fungal yeasts was investigated. Our results revealed that CD18low mice infected with Pb18 survived during the time analyzed; their lungs showed fewer granulomas, a lower fungal load, lower levels of nitrate, and production of high levels of IgG1 in comparison to WT animals. Our results revealed that in vitro macrophages from CD18low mice slowly internalized yeast cells, showing a lower fungal burden compared to WT cells. The migration capacity of macrophages was compromised and showed a higher intensity in the lysosome signal when compared with WT mice. Our data suggest that β2 integrins play an important role in fungal survival inside macrophages, and once phagocytosed, the macrophage may serve as a protective environment for P. brasiliensis. |
| first_indexed | 2024-12-19T12:06:13Z |
| format | Article |
| id | doaj.art-394401e84dfa4b7cb188905d66331de1 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-19T12:06:13Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-394401e84dfa4b7cb188905d66331de12022-12-21T20:22:20ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-03-011110.3389/fcimb.2021.622899622899β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in MiceStephan Alberto Machado de Oliveira0Stephan Alberto Machado de Oliveira1Janayna Nunes Reis2Elisa Catão3Andre Correa Amaral4Ana Camila Oliveira Souza5Alice Melo Ribeiro6Lúcia Helena Faccioli7Fabiana Pirani Carneiro8Clara Luna Freitas Marina9Pedro Henrique Bürgel10Larissa Fernandes11Aldo Henrique Tavares12Anamelia Lorenzetti Bocca13Anamelia Lorenzetti Bocca14Anamelia Lorenzetti Bocca15Molecular Pathology Graduation Course, Faculty of Medicine, University of Brasilia, Brasilia, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilMolecular Pathology Graduation Course, Faculty of Medicine, University of Brasilia, Brasilia, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilInstitute of Tropical Pathology and Public Health, Federal University of Goiás, Goiania, BrazilMolecular Pathology Graduation Course, Faculty of Medicine, University of Brasilia, Brasilia, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilFaculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, BrazilArea of Pathology, Faculty of Medicine, University of Brasilia, Brasilia, BrazilMolecular Biology Graduation Course, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilMolecular Biology Graduation Course, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilFaculty of Ceilândia, University of Brasília, Brasília, BrazilFaculty of Ceilândia, University of Brasília, Brasília, BrazilMolecular Pathology Graduation Course, Faculty of Medicine, University of Brasilia, Brasilia, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilMolecular Biology Graduation Course, Institute of Biological Sciences, University of Brasilia, Brasilia, BrazilThe earliest interaction between macrophages and Paracoccidioides brasiliensis is particularly important in paracoccidioidomycosis (PCM) progression, and surface proteins play a central role in this process. The present study investigated the contribution of β2 integrin in P. brasiliensis-macrophage interaction and PCM progression. We infected β2-low expression (CD18low) and wild type (WT) mice with P. brasiliensis 18. Disease progression was evaluated for fungal burden, lung granulomatous lesions, nitrate levels, and serum antibody production. Besides, the in vitro capacity of macrophages to internalize and kill fungal yeasts was investigated. Our results revealed that CD18low mice infected with Pb18 survived during the time analyzed; their lungs showed fewer granulomas, a lower fungal load, lower levels of nitrate, and production of high levels of IgG1 in comparison to WT animals. Our results revealed that in vitro macrophages from CD18low mice slowly internalized yeast cells, showing a lower fungal burden compared to WT cells. The migration capacity of macrophages was compromised and showed a higher intensity in the lysosome signal when compared with WT mice. Our data suggest that β2 integrins play an important role in fungal survival inside macrophages, and once phagocytosed, the macrophage may serve as a protective environment for P. brasiliensis.https://www.frontiersin.org/articles/10.3389/fcimb.2021.622899/fullCD18low micenitric oxideβ2 integrinParacoccoidioides brasiliensissusceptibility |
| spellingShingle | Stephan Alberto Machado de Oliveira Stephan Alberto Machado de Oliveira Janayna Nunes Reis Elisa Catão Andre Correa Amaral Ana Camila Oliveira Souza Alice Melo Ribeiro Lúcia Helena Faccioli Fabiana Pirani Carneiro Clara Luna Freitas Marina Pedro Henrique Bürgel Larissa Fernandes Aldo Henrique Tavares Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca Anamelia Lorenzetti Bocca β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice Frontiers in Cellular and Infection Microbiology CD18low mice nitric oxide β2 integrin Paracoccoidioides brasiliensis susceptibility |
| title | β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice |
| title_full | β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice |
| title_fullStr | β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice |
| title_full_unstemmed | β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice |
| title_short | β2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice |
| title_sort | β2 integrin mediated susceptibility to paracoccidioides brasiliensis experimental infection in mice |
| topic | CD18low mice nitric oxide β2 integrin Paracoccoidioides brasiliensis susceptibility |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.622899/full |
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