Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report
Severe mucocutaneous (MCL) and diffuse (DCL) forms of American cutaneous leishmaniasis (ACL) are infrequent in Venezuela. Chemotherapy produces only transitory remission in DCL, and occasional treatment failures are observed in MCL. We have evaluated therapy with an experimental vaccine in patients...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2004-02-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100010 |
| _version_ | 1797721265446846464 |
|---|---|
| author | Jacinto Convit Marian Ulrich María Argelia Polegre Angela Avila Noris Rodríguez Maria Ilina Mazzedo Belkis Blanco |
| author_facet | Jacinto Convit Marian Ulrich María Argelia Polegre Angela Avila Noris Rodríguez Maria Ilina Mazzedo Belkis Blanco |
| author_sort | Jacinto Convit |
| collection | DOAJ |
| description | Severe mucocutaneous (MCL) and diffuse (DCL) forms of American cutaneous leishmaniasis (ACL) are infrequent in Venezuela. Chemotherapy produces only transitory remission in DCL, and occasional treatment failures are observed in MCL. We have evaluated therapy with an experimental vaccine in patients with severe leishmaniasis. Four patients with MCL and 3 with early DCL were treated with monthly intradermal injections of a vaccine containing promastigotes of Leishmania (Viannia) braziliensis killed by pasteurization and viable Bacillus Calmette- Guerin. Clinical and immunological responses were evaluated. Integrity of protein constituents in extracts of pasteurized promastigotes was evaluated by gel electrophoresis. Complete remission of lesions occurred after 5-9 injections in patients with MCL or 7-10 injections in patients with early DCL. DCL patients developed positive skin reactions, average size 18.7 mm. All have been free of active lesions for at least 10 months. Adverse effects of the vaccine were limited to local reactivity to BCG at the injection sites and fever in 2 patients. Extracts of pasteurized and fresh promastigotes did not reveal differences in the integrity of protein components detectable by gel electrophoresis. Immunotherapy with this modified vaccine offers an effective, safe option for the treatment of patients who do not respond to immunotherapy with vaccine containing autoclaved parasites or to chemotherapy . |
| first_indexed | 2024-03-12T09:31:01Z |
| format | Article |
| id | doaj.art-394522b032084438af2b7760ec0bff22 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:01Z |
| publishDate | 2004-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-394522b032084438af2b7760ec0bff222023-09-02T13:54:11ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-02-01991576210.1590/S0074-02762004000100010Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary reportJacinto ConvitMarian UlrichMaría Argelia PolegreAngela AvilaNoris RodríguezMaria Ilina MazzedoBelkis BlancoSevere mucocutaneous (MCL) and diffuse (DCL) forms of American cutaneous leishmaniasis (ACL) are infrequent in Venezuela. Chemotherapy produces only transitory remission in DCL, and occasional treatment failures are observed in MCL. We have evaluated therapy with an experimental vaccine in patients with severe leishmaniasis. Four patients with MCL and 3 with early DCL were treated with monthly intradermal injections of a vaccine containing promastigotes of Leishmania (Viannia) braziliensis killed by pasteurization and viable Bacillus Calmette- Guerin. Clinical and immunological responses were evaluated. Integrity of protein constituents in extracts of pasteurized promastigotes was evaluated by gel electrophoresis. Complete remission of lesions occurred after 5-9 injections in patients with MCL or 7-10 injections in patients with early DCL. DCL patients developed positive skin reactions, average size 18.7 mm. All have been free of active lesions for at least 10 months. Adverse effects of the vaccine were limited to local reactivity to BCG at the injection sites and fever in 2 patients. Extracts of pasteurized and fresh promastigotes did not reveal differences in the integrity of protein components detectable by gel electrophoresis. Immunotherapy with this modified vaccine offers an effective, safe option for the treatment of patients who do not respond to immunotherapy with vaccine containing autoclaved parasites or to chemotherapy .http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100010immunotherapydiffuse cutaneous leishmaniasismucocutaneous leishmaniasis |
| spellingShingle | Jacinto Convit Marian Ulrich María Argelia Polegre Angela Avila Noris Rodríguez Maria Ilina Mazzedo Belkis Blanco Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report Memorias do Instituto Oswaldo Cruz immunotherapy diffuse cutaneous leishmaniasis mucocutaneous leishmaniasis |
| title | Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report |
| title_full | Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report |
| title_fullStr | Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report |
| title_full_unstemmed | Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report |
| title_short | Therapy of Venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized Leishmania promastigotes and bacillus Calmette-Guerin: preliminary report |
| title_sort | therapy of venezuelan patients with severe mucocutaneous or early lesions of diffuse cutaneous leishmaniasis with a vaccine containing pasteurized leishmania promastigotes and bacillus calmette guerin preliminary report |
| topic | immunotherapy diffuse cutaneous leishmaniasis mucocutaneous leishmaniasis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000100010 |
| work_keys_str_mv | AT jacintoconvit therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT marianulrich therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT mariaargeliapolegre therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT angelaavila therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT norisrodriguez therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT mariailinamazzedo therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport AT belkisblanco therapyofvenezuelanpatientswithseveremucocutaneousorearlylesionsofdiffusecutaneousleishmaniasiswithavaccinecontainingpasteurizedleishmaniapromastigotesandbacilluscalmetteguerinpreliminaryreport |