The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems
This study aimed to investigate the mechanism of different treatments, namely, ultrasound (US), chlorogenic acid (CA), and ultrasound combined with chlorogenic acid (US plus CA) on the inactivation of Staphylococcus aureus planktonic and biofilm cells. Results showed that the combined treatment of U...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417721003436 |
| _version_ | 1818998487575953408 |
|---|---|
| author | Jinyue Sun Debao Wang Zhilan Sun Fang Liu Lihui Du Daoying Wang |
| author_facet | Jinyue Sun Debao Wang Zhilan Sun Fang Liu Lihui Du Daoying Wang |
| author_sort | Jinyue Sun |
| collection | DOAJ |
| description | This study aimed to investigate the mechanism of different treatments, namely, ultrasound (US), chlorogenic acid (CA), and ultrasound combined with chlorogenic acid (US plus CA) on the inactivation of Staphylococcus aureus planktonic and biofilm cells. Results showed that the combined treatment of US and CA exhibited remarkable synergistic antibacterial and antibiofilm effects. Scanning electron microscopy images indicated that the combined treatment of US and CA caused the most serious damage to the cell morphology. Confocal laser scanning microscopy images revealed that the combined treatment led to sharp increase and severe damage to the permeability of the cell membrane, causing the release of ATP and nucleic acids and decreasing the exopolysaccharide contents in S. aureus biofilm. Finally, the combined treatment of US plus 1% CA for 60 min inactivated S. aureus cells by 1.13 lg CFU/g on mutton. Thus, the combined treatment of US and CA had synergistic effect against S. aureus under planktonic, biofilm, and food systems. |
| first_indexed | 2024-12-20T22:02:19Z |
| format | Article |
| id | doaj.art-394b1229a9ac4e749cb56f6a84e97ab8 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-12-20T22:02:19Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-394b1229a9ac4e749cb56f6a84e97ab82022-12-21T19:25:20ZengElsevierUltrasonics Sonochemistry1350-41772021-12-0180105801The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systemsJinyue Sun0Debao Wang1Zhilan Sun2Fang Liu3Lihui Du4Daoying Wang5Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Corresponding author at: Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China. (F. Liu).College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China; Corresponding author at: Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China. (F. Liu).Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaThis study aimed to investigate the mechanism of different treatments, namely, ultrasound (US), chlorogenic acid (CA), and ultrasound combined with chlorogenic acid (US plus CA) on the inactivation of Staphylococcus aureus planktonic and biofilm cells. Results showed that the combined treatment of US and CA exhibited remarkable synergistic antibacterial and antibiofilm effects. Scanning electron microscopy images indicated that the combined treatment of US and CA caused the most serious damage to the cell morphology. Confocal laser scanning microscopy images revealed that the combined treatment led to sharp increase and severe damage to the permeability of the cell membrane, causing the release of ATP and nucleic acids and decreasing the exopolysaccharide contents in S. aureus biofilm. Finally, the combined treatment of US plus 1% CA for 60 min inactivated S. aureus cells by 1.13 lg CFU/g on mutton. Thus, the combined treatment of US and CA had synergistic effect against S. aureus under planktonic, biofilm, and food systems.http://www.sciencedirect.com/science/article/pii/S1350417721003436S. aureusPlanktonic cellsBiofilmUltrasoundChlorogenic acid |
| spellingShingle | Jinyue Sun Debao Wang Zhilan Sun Fang Liu Lihui Du Daoying Wang The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems Ultrasonics Sonochemistry S. aureus Planktonic cells Biofilm Ultrasound Chlorogenic acid |
| title | The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems |
| title_full | The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems |
| title_fullStr | The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems |
| title_full_unstemmed | The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems |
| title_short | The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems |
| title_sort | combination of ultrasound and chlorogenic acid to inactivate staphylococcus aureus under planktonic biofilm and food systems |
| topic | S. aureus Planktonic cells Biofilm Ultrasound Chlorogenic acid |
| url | http://www.sciencedirect.com/science/article/pii/S1350417721003436 |
| work_keys_str_mv | AT jinyuesun thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT debaowang thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT zhilansun thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT fangliu thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT lihuidu thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT daoyingwang thecombinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT jinyuesun combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT debaowang combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT zhilansun combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT fangliu combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT lihuidu combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems AT daoyingwang combinationofultrasoundandchlorogenicacidtoinactivatestaphylococcusaureusunderplanktonicbiofilmandfoodsystems |