Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort
Background: There has been increasing recognition of the importance of the gut microbiome in Parkinson’s disease (PD), but the influence of geographic location has received little attention. The present study characterized the gut microbiota and associated changes in host metabolic pathways in an Au...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-10-01
|
| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2021.756951/full |
| _version_ | 1819031004646473728 |
|---|---|
| author | Jade E. Kenna Jade E. Kenna Jade E. Kenna Jade E. Kenna Eng Guan Chua Eng Guan Chua Megan Bakeberg Megan Bakeberg Alfred Tay Alfred Tay Sarah McGregor Anastazja Gorecki Anastazja Gorecki Malcolm Horne Malcolm Horne Barry Marshall Barry Marshall Frank L. Mastaglia Frank L. Mastaglia Ryan S. Anderton Ryan S. Anderton Ryan S. Anderton |
| author_facet | Jade E. Kenna Jade E. Kenna Jade E. Kenna Jade E. Kenna Eng Guan Chua Eng Guan Chua Megan Bakeberg Megan Bakeberg Alfred Tay Alfred Tay Sarah McGregor Anastazja Gorecki Anastazja Gorecki Malcolm Horne Malcolm Horne Barry Marshall Barry Marshall Frank L. Mastaglia Frank L. Mastaglia Ryan S. Anderton Ryan S. Anderton Ryan S. Anderton |
| author_sort | Jade E. Kenna |
| collection | DOAJ |
| description | Background: There has been increasing recognition of the importance of the gut microbiome in Parkinson’s disease (PD), but the influence of geographic location has received little attention. The present study characterized the gut microbiota and associated changes in host metabolic pathways in an Australian cohort of people with PD (PwP).Methods: The study involved recruitment and assessment of 87 PwP from multiple Movement Disorders Clinics in Australia and 47 healthy controls. Illumina sequencing of the V3 and V4 regions of the 16S rRNA gene was used to distinguish inter-cohort differences in gut microbiota; KEGG analysis was subsequently performed to predict functional changes in host metabolic pathways.Results: The current findings identified significant differences in relative abundance and diversity of microbial operational taxonomic units (OTUs), and specific bacterial taxa between PwP and control groups. Alpha diversity was significantly reduced in PwP when compared to controls. Differences were found in two phyla (Synergistetes and Proteobacteria; both increased in PwP), and five genera (Colidextribacter, Intestinibacter, Kineothrix, Agathobaculum, and Roseburia; all decreased in PwP). Within the PD cohort, there was no association identified between microbial composition and gender, constipation or use of gastrointestinal medication. Furthermore, KEGG analysis identified 15 upregulated and 11 downregulated metabolic pathways which were predicted to be significantly altered in PwP.Conclusion: This study provides the first comprehensive characterization of the gut microbiome and predicted functional metabolic effects in a southern hemisphere PD population, further exploring the possible mechanisms whereby the gut microbiota may exert their influence on this disease, and providing evidence for the incorporation of such data in future individualized therapeutic strategies. |
| first_indexed | 2024-12-21T06:39:09Z |
| format | Article |
| id | doaj.art-394bda038c6b4117bac8e9330fc90019 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-21T06:39:09Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-394bda038c6b4117bac8e9330fc900192022-12-21T19:12:45ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-10-011510.3389/fnins.2021.756951756951Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease CohortJade E. Kenna0Jade E. Kenna1Jade E. Kenna2Jade E. Kenna3Eng Guan Chua4Eng Guan Chua5Megan Bakeberg6Megan Bakeberg7Alfred Tay8Alfred Tay9Sarah McGregor10Anastazja Gorecki11Anastazja Gorecki12Malcolm Horne13Malcolm Horne14Barry Marshall15Barry Marshall16Frank L. Mastaglia17Frank L. Mastaglia18Ryan S. Anderton19Ryan S. Anderton20Ryan S. Anderton21School of Medicine, The University of Western Australia, Nedlands, WA, AustraliaCentre for Neuromuscular and Neurological Disorders, The University of Western Australia, Nedlands, WA, AustraliaCentre for Clinical Neurosciences and Neurological Research, St. Vincent’s Hospital Melbourne, Fitzroy, VIC, AustraliaPerron Institute for Neurological and Translational Science, Nedlands, WA, AustraliaSchool of Biological Sciences, The University of Western Australia, Crawley, WA, AustraliaMarshall Centre for Infectious Diseases Research and Training, The University of Western Australia, Nedlands, WA, AustraliaCentre for Neuromuscular and Neurological Disorders, The University of Western Australia, Nedlands, WA, AustraliaSchool of Medicine, University of Notre Dame Australia, Fremantle, WA, AustraliaSchool of Biological Sciences, The University of Western Australia, Crawley, WA, AustraliaMarshall Centre for Infectious Diseases Research and Training, The University of Western Australia, Nedlands, WA, AustraliaCentre for Clinical Neurosciences and Neurological Research, St. Vincent’s Hospital Melbourne, Fitzroy, VIC, AustraliaPerron Institute for Neurological and Translational Science, Nedlands, WA, AustraliaSchool of Biological Sciences, The University of Western Australia, Crawley, WA, AustraliaCentre for Clinical Neurosciences and Neurological Research, St. Vincent’s Hospital Melbourne, Fitzroy, VIC, AustraliaFlorey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, AustraliaSchool of Biological Sciences, The University of Western Australia, Crawley, WA, AustraliaMarshall Centre for Infectious Diseases Research and Training, The University of Western Australia, Nedlands, WA, AustraliaCentre for Neuromuscular and Neurological Disorders, The University of Western Australia, Nedlands, WA, AustraliaPerron Institute for Neurological and Translational Science, Nedlands, WA, AustraliaCentre for Neuromuscular and Neurological Disorders, The University of Western Australia, Nedlands, WA, AustraliaInstitute for Health Research, University of Notre Dame Australia, Fremantle, WA, Australia0School of Nursing, Midwifery, Health Sciences and Physiotherapy, The University of Notre Dame Australia, Fremantle, WA, AustraliaBackground: There has been increasing recognition of the importance of the gut microbiome in Parkinson’s disease (PD), but the influence of geographic location has received little attention. The present study characterized the gut microbiota and associated changes in host metabolic pathways in an Australian cohort of people with PD (PwP).Methods: The study involved recruitment and assessment of 87 PwP from multiple Movement Disorders Clinics in Australia and 47 healthy controls. Illumina sequencing of the V3 and V4 regions of the 16S rRNA gene was used to distinguish inter-cohort differences in gut microbiota; KEGG analysis was subsequently performed to predict functional changes in host metabolic pathways.Results: The current findings identified significant differences in relative abundance and diversity of microbial operational taxonomic units (OTUs), and specific bacterial taxa between PwP and control groups. Alpha diversity was significantly reduced in PwP when compared to controls. Differences were found in two phyla (Synergistetes and Proteobacteria; both increased in PwP), and five genera (Colidextribacter, Intestinibacter, Kineothrix, Agathobaculum, and Roseburia; all decreased in PwP). Within the PD cohort, there was no association identified between microbial composition and gender, constipation or use of gastrointestinal medication. Furthermore, KEGG analysis identified 15 upregulated and 11 downregulated metabolic pathways which were predicted to be significantly altered in PwP.Conclusion: This study provides the first comprehensive characterization of the gut microbiome and predicted functional metabolic effects in a southern hemisphere PD population, further exploring the possible mechanisms whereby the gut microbiota may exert their influence on this disease, and providing evidence for the incorporation of such data in future individualized therapeutic strategies.https://www.frontiersin.org/articles/10.3389/fnins.2021.756951/fullParkinson’s diseasegut microbiome16Sgut bacteriaKEGG |
| spellingShingle | Jade E. Kenna Jade E. Kenna Jade E. Kenna Jade E. Kenna Eng Guan Chua Eng Guan Chua Megan Bakeberg Megan Bakeberg Alfred Tay Alfred Tay Sarah McGregor Anastazja Gorecki Anastazja Gorecki Malcolm Horne Malcolm Horne Barry Marshall Barry Marshall Frank L. Mastaglia Frank L. Mastaglia Ryan S. Anderton Ryan S. Anderton Ryan S. Anderton Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort Frontiers in Neuroscience Parkinson’s disease gut microbiome 16S gut bacteria KEGG |
| title | Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort |
| title_full | Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort |
| title_fullStr | Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort |
| title_full_unstemmed | Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort |
| title_short | Changes in the Gut Microbiome and Predicted Functional Metabolic Effects in an Australian Parkinson’s Disease Cohort |
| title_sort | changes in the gut microbiome and predicted functional metabolic effects in an australian parkinson s disease cohort |
| topic | Parkinson’s disease gut microbiome 16S gut bacteria KEGG |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2021.756951/full |
| work_keys_str_mv | AT jadeekenna changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT jadeekenna changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT jadeekenna changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT jadeekenna changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT engguanchua changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT engguanchua changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT meganbakeberg changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT meganbakeberg changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT alfredtay changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT alfredtay changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT sarahmcgregor changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT anastazjagorecki changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT anastazjagorecki changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT malcolmhorne changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT malcolmhorne changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT barrymarshall changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT barrymarshall changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT franklmastaglia changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT franklmastaglia changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT ryansanderton changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT ryansanderton changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort AT ryansanderton changesinthegutmicrobiomeandpredictedfunctionalmetaboliceffectsinanaustralianparkinsonsdiseasecohort |