Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis
ABSTRACT: Proteins are considered major effectors of sperm function. However, the proteins expressed in pigeon sperm have not been explored. Here, we collected semen from meat and racing pigeons using the electroejaculation method and identified proteins in pigeon sperm using the proteomics approach...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123006077 |
| _version_ | 1797660097971748864 |
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| author | Xun Wang Xingyu Li Fuxing Yang Ling Zhao Cheng Meng Yuhao Gao Hongrui Guo Xiaohui Si Haifeng Liu Anan Jiang Xuewei Li |
| author_facet | Xun Wang Xingyu Li Fuxing Yang Ling Zhao Cheng Meng Yuhao Gao Hongrui Guo Xiaohui Si Haifeng Liu Anan Jiang Xuewei Li |
| author_sort | Xun Wang |
| collection | DOAJ |
| description | ABSTRACT: Proteins are considered major effectors of sperm function. However, the proteins expressed in pigeon sperm have not been explored. Here, we collected semen from meat and racing pigeons using the electroejaculation method and identified proteins in pigeon sperm using the proteomics approach. A total of 1,641 proteins were identified in the sperm of domesticated pigeons. Of which, 1,541 proteins were reliably quantified, and gene ontology (GO) and associated bioinformatics analyses indicated that annotated proteins were linked to the oxidation-reduction process, integral component membrane, and protein binding, etc. Among quantified proteins, 1,515 and 1,507 proteins were respectively presented in White King pigeons and racing pigeons, and 1,481 proteins were shared between these 2 types of pigeons, including axonemal dynein, solute carrier, cilia- and flagella-associated protein, outer dense fiber protein, etc. Proteins in our constructed protein-protein interaction (PPI) network are involved in oxidative phosphorylation, sperm axoneme assembly, cilium-dependent cell motility, axonemal dynein complex assembly, flagellated sperm motility, etc. In conclusion, this study characterized the sperm proteome of pigeons and provided a foundation for the subsequent research screening markers for fertility evaluation of pigeons. |
| first_indexed | 2024-03-11T18:24:51Z |
| format | Article |
| id | doaj.art-394cc40d5f0648a4ae8912ac26fa2059 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T18:24:51Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-394cc40d5f0648a4ae8912ac26fa20592023-10-14T04:44:01ZengElsevierPoultry Science0032-57912023-11-0110211103088Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysisXun Wang0Xingyu Li1Fuxing Yang2Ling Zhao3Cheng Meng4Yuhao Gao5Hongrui Guo6Xiaohui Si7Haifeng Liu8Anan Jiang9Xuewei Li10College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Livestock and Poultry Multi-omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Corresponding author:College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Livestock and Poultry Multi-omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Livestock and Poultry Multi-omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Livestock and Poultry Multi-omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; Livestock and Poultry Multi-omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR ChinaABSTRACT: Proteins are considered major effectors of sperm function. However, the proteins expressed in pigeon sperm have not been explored. Here, we collected semen from meat and racing pigeons using the electroejaculation method and identified proteins in pigeon sperm using the proteomics approach. A total of 1,641 proteins were identified in the sperm of domesticated pigeons. Of which, 1,541 proteins were reliably quantified, and gene ontology (GO) and associated bioinformatics analyses indicated that annotated proteins were linked to the oxidation-reduction process, integral component membrane, and protein binding, etc. Among quantified proteins, 1,515 and 1,507 proteins were respectively presented in White King pigeons and racing pigeons, and 1,481 proteins were shared between these 2 types of pigeons, including axonemal dynein, solute carrier, cilia- and flagella-associated protein, outer dense fiber protein, etc. Proteins in our constructed protein-protein interaction (PPI) network are involved in oxidative phosphorylation, sperm axoneme assembly, cilium-dependent cell motility, axonemal dynein complex assembly, flagellated sperm motility, etc. In conclusion, this study characterized the sperm proteome of pigeons and provided a foundation for the subsequent research screening markers for fertility evaluation of pigeons.http://www.sciencedirect.com/science/article/pii/S0032579123006077meat pigeonracing pigeonspermatozoaproteome |
| spellingShingle | Xun Wang Xingyu Li Fuxing Yang Ling Zhao Cheng Meng Yuhao Gao Hongrui Guo Xiaohui Si Haifeng Liu Anan Jiang Xuewei Li Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis Poultry Science meat pigeon racing pigeon spermatozoa proteome |
| title | Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| title_full | Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| title_fullStr | Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| title_full_unstemmed | Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| title_short | Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| title_sort | research note spermatozoa proteins identification in domesticated pigeons by proteomic analysis |
| topic | meat pigeon racing pigeon spermatozoa proteome |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123006077 |
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