Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling
<i>Annona glabra</i> L. (AngTE) and <i>Annona squamosa</i> L. (AnsTE) fruits have been widely used in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling...
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MDPI AG
2022-11-01
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| Series: | Pharmaceuticals |
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| author | Fatma A. Mokhtar Nabil M. Selim Seham S. Elhawary Soha R. Abd El Hadi Mona H. Hetta Marzough A. Albalawi Ali A. Shati Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Lamiaa I. Fahmy Rana M. Ibrahim |
| author_facet | Fatma A. Mokhtar Nabil M. Selim Seham S. Elhawary Soha R. Abd El Hadi Mona H. Hetta Marzough A. Albalawi Ali A. Shati Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Lamiaa I. Fahmy Rana M. Ibrahim |
| author_sort | Fatma A. Mokhtar |
| collection | DOAJ |
| description | <i>Annona glabra</i> L. (AngTE) and <i>Annona squamosa</i> L. (AnsTE) fruits have been widely used in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling was established using UPLC-QTOF-MS/MS. All species were tested for anticancer activity against human cervical cancer cells (HeLa), prostate adenocarcinoma metastatic (PC3), and ovary adenocarcinoma (SKOV3) using sulphorhodamine B assay. Apoptosis was determined using Annexin flow cytometry along with cell cycle analysis and supported by a molecular docking. The antibacterial and synergistic effect when combined with gentamicin were evaluated. A total of 114 compounds were tentatively identified, mainly acetogenins and <i>ent</i>-kaurane diterpenes. AnsTE and Ans-AgNPs had the most potent cytotoxicity on HeLa and SKOV3 cells, inducing a significant apoptotic effect against all tumor cells. The AnsTE and Ans-AgNPs significantly arrested PC3, SKOV3, and HeLa cells in the S phase. The nanoparticles demonstrated greater antibacterial and antifungal activities, as well as a synergistic effect with gentamicin against <i>P. aeruginosa</i> and <i>E. coli</i>. Finally, a molecular docking was attempted to investigate the binding mode of the identified compounds in Bcl-2 proteins’ receptor, implying that the fruits and their nanoparticles are excellent candidates for treating skin infections in patients with ovarian or prostatic cancer. |
| first_indexed | 2024-03-09T18:44:51Z |
| format | Article |
| id | doaj.art-394e874e60cf43ab9a6747e918cd1731 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T18:44:51Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-394e874e60cf43ab9a6747e918cd17312023-11-24T06:18:36ZengMDPI AGPharmaceuticals1424-82472022-11-011511135410.3390/ph15111354Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic ProfilingFatma A. Mokhtar0Nabil M. Selim1Seham S. Elhawary2Soha R. Abd El Hadi3Mona H. Hetta4Marzough A. Albalawi5Ali A. Shati6Mohammad Y. Alfaifi7Serag Eldin I. Elbehairi8Lamiaa I. Fahmy9Rana M. Ibrahim10Department of Pharmacognosy, Faculty of Pharmacy, Al Salam University, Kafr Alzayat, Algharbia 31611, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo 11562, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo 11562, EgyptDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo 11562, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Fayoum University, Fayoum 63514, EgyptDepartment of Chemistry, Alwajh College, University of Tabuk, Tabuk 71491, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, Alfra’a 62223, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, Alfra’a 62223, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, Alfra’a 62223, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12573, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo 11562, Egypt<i>Annona glabra</i> L. (AngTE) and <i>Annona squamosa</i> L. (AnsTE) fruits have been widely used in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling was established using UPLC-QTOF-MS/MS. All species were tested for anticancer activity against human cervical cancer cells (HeLa), prostate adenocarcinoma metastatic (PC3), and ovary adenocarcinoma (SKOV3) using sulphorhodamine B assay. Apoptosis was determined using Annexin flow cytometry along with cell cycle analysis and supported by a molecular docking. The antibacterial and synergistic effect when combined with gentamicin were evaluated. A total of 114 compounds were tentatively identified, mainly acetogenins and <i>ent</i>-kaurane diterpenes. AnsTE and Ans-AgNPs had the most potent cytotoxicity on HeLa and SKOV3 cells, inducing a significant apoptotic effect against all tumor cells. The AnsTE and Ans-AgNPs significantly arrested PC3, SKOV3, and HeLa cells in the S phase. The nanoparticles demonstrated greater antibacterial and antifungal activities, as well as a synergistic effect with gentamicin against <i>P. aeruginosa</i> and <i>E. coli</i>. Finally, a molecular docking was attempted to investigate the binding mode of the identified compounds in Bcl-2 proteins’ receptor, implying that the fruits and their nanoparticles are excellent candidates for treating skin infections in patients with ovarian or prostatic cancer.https://www.mdpi.com/1424-8247/15/11/1354<i>Annona</i>UPLC-QTOF-MS/MSsilver nanoparticlesanticancerapoptosisantibacterial |
| spellingShingle | Fatma A. Mokhtar Nabil M. Selim Seham S. Elhawary Soha R. Abd El Hadi Mona H. Hetta Marzough A. Albalawi Ali A. Shati Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Lamiaa I. Fahmy Rana M. Ibrahim Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling Pharmaceuticals <i>Annona</i> UPLC-QTOF-MS/MS silver nanoparticles anticancer apoptosis antibacterial |
| title | Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
| title_full | Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
| title_fullStr | Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
| title_full_unstemmed | Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
| title_short | Green Biosynthesis of Silver Nanoparticles Using <i>Annona glabra</i> and <i>Annona squamosa</i> Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
| title_sort | green biosynthesis of silver nanoparticles using i annona glabra i and i annona squamosa i extracts with antimicrobial anticancer apoptosis potentials assisted by in silico modeling and metabolic profiling |
| topic | <i>Annona</i> UPLC-QTOF-MS/MS silver nanoparticles anticancer apoptosis antibacterial |
| url | https://www.mdpi.com/1424-8247/15/11/1354 |
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