BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i>
<i>Brassica napus</i> (oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by <i>Sclerotinia sclerotiorum</i>. The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA...
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MDPI AG
2022-02-01
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| author | Ka Zhang Chenjian Zhuo Zhixin Wang Fei Liu Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu |
| author_facet | Ka Zhang Chenjian Zhuo Zhixin Wang Fei Liu Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu |
| author_sort | Ka Zhang |
| collection | DOAJ |
| description | <i>Brassica napus</i> (oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by <i>Sclerotinia sclerotiorum</i>. The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by <i>S. sclerotiorum</i> infection was involved in plant immunity. <i>BnaA03.MKK5</i> overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including <i>BnPAD3</i> and <i>BnCYP71A13</i>. In addition, two copies of <i>BnMPK3</i> (<i>BnA06.MPK3</i> and <i>BnC03.MPK3</i>) were induced by <i>Sclerotinia</i> incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPK3 in yeast. These interactions were confirmed using in vivo co-immunoprecipitation assays. In vitro phosphorylation assays showed that BnaA06.MPK3 and BnaC03.MPK3 were the direct phosphorylation substrates of BnaA03.MKK5. The transgenic oilseed rape plants including <i>BnaA06.MPK3</i> and <i>BnaC03.MPK3</i> overexpression lines and <i>BnMPK3</i> gene editing lines mediated by CRISPR/Cas9 were generated; the results of the genetic transformation of <i>BnaA06.MPK3/BnaC03.MPK3</i> indicate that BnMPK3 also has a positive role in <i>Sclerotinia</i> resistance. This study provides information about the potential mechanism of <i>B. napus</i> defense against <i>S. Sclerotiorum</i> mediated by a detailed BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module. |
| first_indexed | 2024-03-09T20:26:00Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T20:26:00Z |
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| spelling | doaj.art-3950f3374fc240fbbc23102d89d4ee162023-11-23T23:35:27ZengMDPI AGPlants2223-77472022-02-0111560910.3390/plants11050609BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i>Ka Zhang0Chenjian Zhuo1Zhixin Wang2Fei Liu3Jing Wen4Bin Yi5Jinxiong Shen6Chaozhi Ma7Tingdong Fu8Jinxing Tu9National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China<i>Brassica napus</i> (oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by <i>Sclerotinia sclerotiorum</i>. The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by <i>S. sclerotiorum</i> infection was involved in plant immunity. <i>BnaA03.MKK5</i> overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including <i>BnPAD3</i> and <i>BnCYP71A13</i>. In addition, two copies of <i>BnMPK3</i> (<i>BnA06.MPK3</i> and <i>BnC03.MPK3</i>) were induced by <i>Sclerotinia</i> incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPK3 in yeast. These interactions were confirmed using in vivo co-immunoprecipitation assays. In vitro phosphorylation assays showed that BnaA06.MPK3 and BnaC03.MPK3 were the direct phosphorylation substrates of BnaA03.MKK5. The transgenic oilseed rape plants including <i>BnaA06.MPK3</i> and <i>BnaC03.MPK3</i> overexpression lines and <i>BnMPK3</i> gene editing lines mediated by CRISPR/Cas9 were generated; the results of the genetic transformation of <i>BnaA06.MPK3/BnaC03.MPK3</i> indicate that BnMPK3 also has a positive role in <i>Sclerotinia</i> resistance. This study provides information about the potential mechanism of <i>B. napus</i> defense against <i>S. Sclerotiorum</i> mediated by a detailed BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module.https://www.mdpi.com/2223-7747/11/5/609<i>Brassica napus</i><i>Sclerotinia sclerotiorum</i>mitogen-activated protein kinaseplant defensemolecular mechanism |
| spellingShingle | Ka Zhang Chenjian Zhuo Zhixin Wang Fei Liu Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> Plants <i>Brassica napus</i> <i>Sclerotinia sclerotiorum</i> mitogen-activated protein kinase plant defense molecular mechanism |
| title | BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> |
| title_full | BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> |
| title_fullStr | BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> |
| title_full_unstemmed | BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> |
| title_short | BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to <i>Sclerotinia sclerotiorum</i> Resistance in <i>Brassica napus</i> |
| title_sort | bnaa03 mkk5 bnaa06 mpk3 bnac03 mpk3 module positively contributes to i sclerotinia sclerotiorum i resistance in i brassica napus i |
| topic | <i>Brassica napus</i> <i>Sclerotinia sclerotiorum</i> mitogen-activated protein kinase plant defense molecular mechanism |
| url | https://www.mdpi.com/2223-7747/11/5/609 |
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