Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry
ObjectiveTo establish an analytical method for 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry （LC-MS/MS） including 5 acute liver failure toxins （α-amanitin， β-amanitin， γ-amanitin， phallacidin and phalloidin） and 4 psycho-neurological disorder toxins （ibotenic aci...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-03-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202202011?st=article_issue |
| _version_ | 1797976841561047040 |
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| author | XU Xiaomin ZHENG Yibin HUANG Baifen XU Jiaojiao CAI Zengxuan HAN Jianlong |
| author_facet | XU Xiaomin ZHENG Yibin HUANG Baifen XU Jiaojiao CAI Zengxuan HAN Jianlong |
| author_sort | XU Xiaomin |
| collection | DOAJ |
| description | ObjectiveTo establish an analytical method for 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry （LC-MS/MS） including 5 acute liver failure toxins （α-amanitin， β-amanitin， γ-amanitin， phallacidin and phalloidin） and 4 psycho-neurological disorder toxins （ibotenic acid， musimol， muscarine and psilocybin）.MethodsThe dried sample was extracted with 50%（V/V）methanol /water mixture containing 0.5%（V/V）formic acid， protein was precipitated with acetonitrile and diluted with water. The toxins were separated with XBridgeTM BEH C18 column （150 mm×3.0 mm， 2.5 μm）， gradient eluted with the mobile phase of 0.005%（V/V） formic acid aqueous solution–methanol， and measured by MS/MS with multiple reaction monitoring （MRM） mode.ResultsThe conditions of sample extraction， dilution ratio and chromatographic separation were optimized. 9 mushroom toxins in different wild fungus could be qualitatively and quantitatively measured based on the pure solvent standard calibration. The limits of detection （LODs） and quantification （LOQs） in sample matrix were 6-15 mg/kg and 20-50 mg/kg， respectively. The linear ranges were 0.004-2 mg/L with correlation coefficients （r） of between 0.9973-0.9995. The average recoveries at three spiking levels （50 mg/kg， 500 mg/kg and 5000 mg/kg） were 78.6%-109.7% with relative standard deviations （RSDs） of 2.7%-9.0%. The toxin compositions and contents varied greatly in the wild mushrooms sampled in some poisoning cases.ConclusionThe method is simple， rapid and accurate. It has been applied to the analysis of mushroom toxins in common poisonous wild mushrooms and the disposal of poisoning emergencies. It can provide rapid and accurate technical support for the etiology identification of poisoning cases and the timely rescue of patients. |
| first_indexed | 2024-04-11T04:57:24Z |
| format | Article |
| id | doaj.art-3950f9985a7840a2ba9c9c219a0b2b3e |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:57:24Z |
| publishDate | 2022-03-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-3950f9985a7840a2ba9c9c219a0b2b3e2022-12-26T07:33:34ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-03-0134226226910.13590/j.cjfh.2022.02.0111004-8456（2022）02-0262-08Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometryXU XiaominZHENG YibinHUANG BaifenXU JiaojiaoCAI ZengxuanHAN JianlongObjectiveTo establish an analytical method for 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry （LC-MS/MS） including 5 acute liver failure toxins （α-amanitin， β-amanitin， γ-amanitin， phallacidin and phalloidin） and 4 psycho-neurological disorder toxins （ibotenic acid， musimol， muscarine and psilocybin）.MethodsThe dried sample was extracted with 50%（V/V）methanol /water mixture containing 0.5%（V/V）formic acid， protein was precipitated with acetonitrile and diluted with water. The toxins were separated with XBridgeTM BEH C18 column （150 mm×3.0 mm， 2.5 μm）， gradient eluted with the mobile phase of 0.005%（V/V） formic acid aqueous solution–methanol， and measured by MS/MS with multiple reaction monitoring （MRM） mode.ResultsThe conditions of sample extraction， dilution ratio and chromatographic separation were optimized. 9 mushroom toxins in different wild fungus could be qualitatively and quantitatively measured based on the pure solvent standard calibration. The limits of detection （LODs） and quantification （LOQs） in sample matrix were 6-15 mg/kg and 20-50 mg/kg， respectively. The linear ranges were 0.004-2 mg/L with correlation coefficients （r） of between 0.9973-0.9995. The average recoveries at three spiking levels （50 mg/kg， 500 mg/kg and 5000 mg/kg） were 78.6%-109.7% with relative standard deviations （RSDs） of 2.7%-9.0%. The toxin compositions and contents varied greatly in the wild mushrooms sampled in some poisoning cases.ConclusionThe method is simple， rapid and accurate. It has been applied to the analysis of mushroom toxins in common poisonous wild mushrooms and the disposal of poisoning emergencies. It can provide rapid and accurate technical support for the etiology identification of poisoning cases and the timely rescue of patients.http://www.zgspws.com/zgspwszz/article/abstract/202202011?st=article_issueliquid chromatography-tandem mass spectrometrymushroom toxinsacute liver failurepsycho-neurological disorderwild fungus |
| spellingShingle | XU Xiaomin ZHENG Yibin HUANG Baifen XU Jiaojiao CAI Zengxuan HAN Jianlong Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry Zhongguo shipin weisheng zazhi liquid chromatography-tandem mass spectrometry mushroom toxins acute liver failure psycho-neurological disorder wild fungus |
| title | Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry |
| title_full | Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry |
| title_fullStr | Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry |
| title_full_unstemmed | Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry |
| title_short | Fast determination of 9 mushroom toxins in wild fungus by liquid chromatography-tandem mass spectrometry |
| title_sort | fast determination of 9 mushroom toxins in wild fungus by liquid chromatography tandem mass spectrometry |
| topic | liquid chromatography-tandem mass spectrometry mushroom toxins acute liver failure psycho-neurological disorder wild fungus |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202202011?st=article_issue |
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